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Ligand-receptor-mediated attachment of lipid vesicles to a supported lipid bilayer

The interaction of exosomes (cell-secreted [Formula: see text] 100 nm-sized extracellular vesicles) or membrane-enveloped virions with cellular lipid membranes is often mediated by relatively weak ligand-receptor bonds. Interactions of this type can be studied using vesicles and observing their atta...

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Autor principal: Zhdanov, Vladimir P.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer International Publishing 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7351846/
https://www.ncbi.nlm.nih.gov/pubmed/32556429
http://dx.doi.org/10.1007/s00249-020-01441-0
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author Zhdanov, Vladimir P.
author_facet Zhdanov, Vladimir P.
author_sort Zhdanov, Vladimir P.
collection PubMed
description The interaction of exosomes (cell-secreted [Formula: see text] 100 nm-sized extracellular vesicles) or membrane-enveloped virions with cellular lipid membranes is often mediated by relatively weak ligand-receptor bonds. Interactions of this type can be studied using vesicles and observing their attachment to receptors located in a lipid bilayer formed at a solid surface. The contact region between a vesicle and the supported lipid bilayer and accordingly the number of ligand-receptor pairs there can be increased by deforming a vesicle. Herein, I (i) estimate theoretically the corresponding deformation energy assuming a disk-like or elongated shape of vesicles, (ii) present the equations allowing one to track such deformations by employing total internal reflection fluorescence microscopy and surface plasmon resonance, and (iii) briefly discuss some related experimental studies.
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spelling pubmed-73518462020-07-14 Ligand-receptor-mediated attachment of lipid vesicles to a supported lipid bilayer Zhdanov, Vladimir P. Eur Biophys J Biophysics Letter The interaction of exosomes (cell-secreted [Formula: see text] 100 nm-sized extracellular vesicles) or membrane-enveloped virions with cellular lipid membranes is often mediated by relatively weak ligand-receptor bonds. Interactions of this type can be studied using vesicles and observing their attachment to receptors located in a lipid bilayer formed at a solid surface. The contact region between a vesicle and the supported lipid bilayer and accordingly the number of ligand-receptor pairs there can be increased by deforming a vesicle. Herein, I (i) estimate theoretically the corresponding deformation energy assuming a disk-like or elongated shape of vesicles, (ii) present the equations allowing one to track such deformations by employing total internal reflection fluorescence microscopy and surface plasmon resonance, and (iii) briefly discuss some related experimental studies. Springer International Publishing 2020-06-17 2020 /pmc/articles/PMC7351846/ /pubmed/32556429 http://dx.doi.org/10.1007/s00249-020-01441-0 Text en © The Author(s) 2020 Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Biophysics Letter
Zhdanov, Vladimir P.
Ligand-receptor-mediated attachment of lipid vesicles to a supported lipid bilayer
title Ligand-receptor-mediated attachment of lipid vesicles to a supported lipid bilayer
title_full Ligand-receptor-mediated attachment of lipid vesicles to a supported lipid bilayer
title_fullStr Ligand-receptor-mediated attachment of lipid vesicles to a supported lipid bilayer
title_full_unstemmed Ligand-receptor-mediated attachment of lipid vesicles to a supported lipid bilayer
title_short Ligand-receptor-mediated attachment of lipid vesicles to a supported lipid bilayer
title_sort ligand-receptor-mediated attachment of lipid vesicles to a supported lipid bilayer
topic Biophysics Letter
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7351846/
https://www.ncbi.nlm.nih.gov/pubmed/32556429
http://dx.doi.org/10.1007/s00249-020-01441-0
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