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author Nitta, Nao
Iino, Takanori
Isozaki, Akihiro
Yamagishi, Mai
Kitahama, Yasutaka
Sakuma, Shinya
Suzuki, Yuta
Tezuka, Hiroshi
Oikawa, Minoru
Arai, Fumihito
Asai, Takuya
Deng, Dinghuan
Fukuzawa, Hideya
Hase, Misa
Hasunuma, Tomohisa
Hayakawa, Takeshi
Hiraki, Kei
Hiramatsu, Kotaro
Hoshino, Yu
Inaba, Mary
Inoue, Yuki
Ito, Takuro
Kajikawa, Masataka
Karakawa, Hiroshi
Kasai, Yusuke
Kato, Yuichi
Kobayashi, Hirofumi
Lei, Cheng
Matsusaka, Satoshi
Mikami, Hideharu
Nakagawa, Atsuhiro
Numata, Keiji
Ota, Tadataka
Sekiya, Takeichiro
Shiba, Kiyotaka
Shirasaki, Yoshitaka
Suzuki, Nobutake
Tanaka, Shunji
Ueno, Shunnosuke
Watarai, Hiroshi
Yamano, Takashi
Yazawa, Masayuki
Yonamine, Yusuke
Di Carlo, Dino
Hosokawa, Yoichiroh
Uemura, Sotaro
Sugimura, Takeaki
Ozeki, Yasuyuki
Goda, Keisuke
author_facet Nitta, Nao
Iino, Takanori
Isozaki, Akihiro
Yamagishi, Mai
Kitahama, Yasutaka
Sakuma, Shinya
Suzuki, Yuta
Tezuka, Hiroshi
Oikawa, Minoru
Arai, Fumihito
Asai, Takuya
Deng, Dinghuan
Fukuzawa, Hideya
Hase, Misa
Hasunuma, Tomohisa
Hayakawa, Takeshi
Hiraki, Kei
Hiramatsu, Kotaro
Hoshino, Yu
Inaba, Mary
Inoue, Yuki
Ito, Takuro
Kajikawa, Masataka
Karakawa, Hiroshi
Kasai, Yusuke
Kato, Yuichi
Kobayashi, Hirofumi
Lei, Cheng
Matsusaka, Satoshi
Mikami, Hideharu
Nakagawa, Atsuhiro
Numata, Keiji
Ota, Tadataka
Sekiya, Takeichiro
Shiba, Kiyotaka
Shirasaki, Yoshitaka
Suzuki, Nobutake
Tanaka, Shunji
Ueno, Shunnosuke
Watarai, Hiroshi
Yamano, Takashi
Yazawa, Masayuki
Yonamine, Yusuke
Di Carlo, Dino
Hosokawa, Yoichiroh
Uemura, Sotaro
Sugimura, Takeaki
Ozeki, Yasuyuki
Goda, Keisuke
author_sort Nitta, Nao
collection PubMed
description The advent of image-activated cell sorting and imaging-based cell picking has advanced our knowledge and exploitation of biological systems in the last decade. Unfortunately, they generally rely on fluorescent labeling for cellular phenotyping, an indirect measure of the molecular landscape in the cell, which has critical limitations. Here we demonstrate Raman image-activated cell sorting by directly probing chemically specific intracellular molecular vibrations via ultrafast multicolor stimulated Raman scattering (SRS) microscopy for cellular phenotyping. Specifically, the technology enables real-time SRS-image-based sorting of single live cells with a throughput of up to ~100 events per second without the need for fluorescent labeling. To show the broad utility of the technology, we show its applicability to diverse cell types and sizes. The technology is highly versatile and holds promise for numerous applications that are previously difficult or undesirable with fluorescence-based technologies.
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spelling pubmed-73519932020-07-16 Raman image-activated cell sorting Nitta, Nao Iino, Takanori Isozaki, Akihiro Yamagishi, Mai Kitahama, Yasutaka Sakuma, Shinya Suzuki, Yuta Tezuka, Hiroshi Oikawa, Minoru Arai, Fumihito Asai, Takuya Deng, Dinghuan Fukuzawa, Hideya Hase, Misa Hasunuma, Tomohisa Hayakawa, Takeshi Hiraki, Kei Hiramatsu, Kotaro Hoshino, Yu Inaba, Mary Inoue, Yuki Ito, Takuro Kajikawa, Masataka Karakawa, Hiroshi Kasai, Yusuke Kato, Yuichi Kobayashi, Hirofumi Lei, Cheng Matsusaka, Satoshi Mikami, Hideharu Nakagawa, Atsuhiro Numata, Keiji Ota, Tadataka Sekiya, Takeichiro Shiba, Kiyotaka Shirasaki, Yoshitaka Suzuki, Nobutake Tanaka, Shunji Ueno, Shunnosuke Watarai, Hiroshi Yamano, Takashi Yazawa, Masayuki Yonamine, Yusuke Di Carlo, Dino Hosokawa, Yoichiroh Uemura, Sotaro Sugimura, Takeaki Ozeki, Yasuyuki Goda, Keisuke Nat Commun Article The advent of image-activated cell sorting and imaging-based cell picking has advanced our knowledge and exploitation of biological systems in the last decade. Unfortunately, they generally rely on fluorescent labeling for cellular phenotyping, an indirect measure of the molecular landscape in the cell, which has critical limitations. Here we demonstrate Raman image-activated cell sorting by directly probing chemically specific intracellular molecular vibrations via ultrafast multicolor stimulated Raman scattering (SRS) microscopy for cellular phenotyping. Specifically, the technology enables real-time SRS-image-based sorting of single live cells with a throughput of up to ~100 events per second without the need for fluorescent labeling. To show the broad utility of the technology, we show its applicability to diverse cell types and sizes. The technology is highly versatile and holds promise for numerous applications that are previously difficult or undesirable with fluorescence-based technologies. Nature Publishing Group UK 2020-07-10 /pmc/articles/PMC7351993/ /pubmed/32651381 http://dx.doi.org/10.1038/s41467-020-17285-3 Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Nitta, Nao
Iino, Takanori
Isozaki, Akihiro
Yamagishi, Mai
Kitahama, Yasutaka
Sakuma, Shinya
Suzuki, Yuta
Tezuka, Hiroshi
Oikawa, Minoru
Arai, Fumihito
Asai, Takuya
Deng, Dinghuan
Fukuzawa, Hideya
Hase, Misa
Hasunuma, Tomohisa
Hayakawa, Takeshi
Hiraki, Kei
Hiramatsu, Kotaro
Hoshino, Yu
Inaba, Mary
Inoue, Yuki
Ito, Takuro
Kajikawa, Masataka
Karakawa, Hiroshi
Kasai, Yusuke
Kato, Yuichi
Kobayashi, Hirofumi
Lei, Cheng
Matsusaka, Satoshi
Mikami, Hideharu
Nakagawa, Atsuhiro
Numata, Keiji
Ota, Tadataka
Sekiya, Takeichiro
Shiba, Kiyotaka
Shirasaki, Yoshitaka
Suzuki, Nobutake
Tanaka, Shunji
Ueno, Shunnosuke
Watarai, Hiroshi
Yamano, Takashi
Yazawa, Masayuki
Yonamine, Yusuke
Di Carlo, Dino
Hosokawa, Yoichiroh
Uemura, Sotaro
Sugimura, Takeaki
Ozeki, Yasuyuki
Goda, Keisuke
Raman image-activated cell sorting
title Raman image-activated cell sorting
title_full Raman image-activated cell sorting
title_fullStr Raman image-activated cell sorting
title_full_unstemmed Raman image-activated cell sorting
title_short Raman image-activated cell sorting
title_sort raman image-activated cell sorting
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7351993/
https://www.ncbi.nlm.nih.gov/pubmed/32651381
http://dx.doi.org/10.1038/s41467-020-17285-3
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