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Intra-Articular Route for the System of Molecules 14G1862 from Centella asiatica: Pain Relieving and Protective Effects in a Rat Model of Osteoarthritis
Current pharmacological therapies for the management of chronic articular diseases are far from being satisfactory, so new strategies need to be investigated. We tested the intra-articular pain relieving properties of a system of molecules from a characterized Centella asiatica extract (14G1862) in...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7352185/ https://www.ncbi.nlm.nih.gov/pubmed/32486519 http://dx.doi.org/10.3390/nu12061618 |
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author | Micheli, Laura Di Cesare Mannelli, Lorenzo Mattoli, Luisa Tamimi, Sara Flamini, Enrico Garetto, Stefano Lucci, Jacopo Giovagnoni, Emiliano Cinci, Lorenzo D’Ambrosio, Mario Luceri, Cristina Ghelardini, Carla |
author_facet | Micheli, Laura Di Cesare Mannelli, Lorenzo Mattoli, Luisa Tamimi, Sara Flamini, Enrico Garetto, Stefano Lucci, Jacopo Giovagnoni, Emiliano Cinci, Lorenzo D’Ambrosio, Mario Luceri, Cristina Ghelardini, Carla |
author_sort | Micheli, Laura |
collection | PubMed |
description | Current pharmacological therapies for the management of chronic articular diseases are far from being satisfactory, so new strategies need to be investigated. We tested the intra-articular pain relieving properties of a system of molecules from a characterized Centella asiatica extract (14G1862) in a rat model of osteoarthritis induced by monoiodoacetate (MIA). 14G1862 (0.2–2 mg mL(−1)) was intra-articularly (i.a.) injected 7 days after MIA, behavioural and histological evaluations were performed 14, 30 and 60 days after treatments. Moreover, the effect of 14G1862 on nitrate production and iNOS expression in RAW 264.7 macrophages stimulated with LPS was assessed. In vitro, 14G1862 treatment attenuated LPS-induced NO production and iNOS expression in a comparable manner to celecoxib. In vivo, 14G1862 significantly reduced mechanical allodynia and hyperalgesia, spontaneous pain and motor alterations starting on day 14 up to day 60. The efficacy was higher or comparable to that evoked by triamcinolone acetonide (100 μg i.a.) used as reference drug. Histological evaluation highlighted the improvement of several morphological parameters in MIA + 14G1862-treated animals with particularly benefic effects on joint space and fibrin deposition. In conclusion, i.a. treatment with Centella asiatica is a candidate to be a novel effective approach for osteoarthritis therapy. |
format | Online Article Text |
id | pubmed-7352185 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-73521852020-07-15 Intra-Articular Route for the System of Molecules 14G1862 from Centella asiatica: Pain Relieving and Protective Effects in a Rat Model of Osteoarthritis Micheli, Laura Di Cesare Mannelli, Lorenzo Mattoli, Luisa Tamimi, Sara Flamini, Enrico Garetto, Stefano Lucci, Jacopo Giovagnoni, Emiliano Cinci, Lorenzo D’Ambrosio, Mario Luceri, Cristina Ghelardini, Carla Nutrients Article Current pharmacological therapies for the management of chronic articular diseases are far from being satisfactory, so new strategies need to be investigated. We tested the intra-articular pain relieving properties of a system of molecules from a characterized Centella asiatica extract (14G1862) in a rat model of osteoarthritis induced by monoiodoacetate (MIA). 14G1862 (0.2–2 mg mL(−1)) was intra-articularly (i.a.) injected 7 days after MIA, behavioural and histological evaluations were performed 14, 30 and 60 days after treatments. Moreover, the effect of 14G1862 on nitrate production and iNOS expression in RAW 264.7 macrophages stimulated with LPS was assessed. In vitro, 14G1862 treatment attenuated LPS-induced NO production and iNOS expression in a comparable manner to celecoxib. In vivo, 14G1862 significantly reduced mechanical allodynia and hyperalgesia, spontaneous pain and motor alterations starting on day 14 up to day 60. The efficacy was higher or comparable to that evoked by triamcinolone acetonide (100 μg i.a.) used as reference drug. Histological evaluation highlighted the improvement of several morphological parameters in MIA + 14G1862-treated animals with particularly benefic effects on joint space and fibrin deposition. In conclusion, i.a. treatment with Centella asiatica is a candidate to be a novel effective approach for osteoarthritis therapy. MDPI 2020-05-31 /pmc/articles/PMC7352185/ /pubmed/32486519 http://dx.doi.org/10.3390/nu12061618 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Micheli, Laura Di Cesare Mannelli, Lorenzo Mattoli, Luisa Tamimi, Sara Flamini, Enrico Garetto, Stefano Lucci, Jacopo Giovagnoni, Emiliano Cinci, Lorenzo D’Ambrosio, Mario Luceri, Cristina Ghelardini, Carla Intra-Articular Route for the System of Molecules 14G1862 from Centella asiatica: Pain Relieving and Protective Effects in a Rat Model of Osteoarthritis |
title | Intra-Articular Route for the System of Molecules 14G1862 from Centella asiatica: Pain Relieving and Protective Effects in a Rat Model of Osteoarthritis |
title_full | Intra-Articular Route for the System of Molecules 14G1862 from Centella asiatica: Pain Relieving and Protective Effects in a Rat Model of Osteoarthritis |
title_fullStr | Intra-Articular Route for the System of Molecules 14G1862 from Centella asiatica: Pain Relieving and Protective Effects in a Rat Model of Osteoarthritis |
title_full_unstemmed | Intra-Articular Route for the System of Molecules 14G1862 from Centella asiatica: Pain Relieving and Protective Effects in a Rat Model of Osteoarthritis |
title_short | Intra-Articular Route for the System of Molecules 14G1862 from Centella asiatica: Pain Relieving and Protective Effects in a Rat Model of Osteoarthritis |
title_sort | intra-articular route for the system of molecules 14g1862 from centella asiatica: pain relieving and protective effects in a rat model of osteoarthritis |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7352185/ https://www.ncbi.nlm.nih.gov/pubmed/32486519 http://dx.doi.org/10.3390/nu12061618 |
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