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Mapping Fluorescence Enhancement of Plasmonic Nanorod Coupled Dye Molecules

Plasmonically enhanced fluorescence is a widely studied and applied phenomenon, however, only a comparative theoretical and experimental analysis of coupled fluorophores and plasmonic nanoresonators makes it possible to uncover how this phenomenon can be controlled. A numerical optimization method w...

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Autores principales: Tóth, Emese, Ungor, Ditta, Novák, Tibor, Ferenc, Györgyi, Bánhelyi, Balázs, Csapó, Edit, Erdélyi, Miklós, Csete, Mária
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7352240/
https://www.ncbi.nlm.nih.gov/pubmed/32485951
http://dx.doi.org/10.3390/nano10061048
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author Tóth, Emese
Ungor, Ditta
Novák, Tibor
Ferenc, Györgyi
Bánhelyi, Balázs
Csapó, Edit
Erdélyi, Miklós
Csete, Mária
author_facet Tóth, Emese
Ungor, Ditta
Novák, Tibor
Ferenc, Györgyi
Bánhelyi, Balázs
Csapó, Edit
Erdélyi, Miklós
Csete, Mária
author_sort Tóth, Emese
collection PubMed
description Plasmonically enhanced fluorescence is a widely studied and applied phenomenon, however, only a comparative theoretical and experimental analysis of coupled fluorophores and plasmonic nanoresonators makes it possible to uncover how this phenomenon can be controlled. A numerical optimization method was applied to design configurations that are capable of resulting in an enhancement of excitation and emission, moreover, of both phenomena simultaneously in coupled Cy5 dye molecule and gold nanorod systems. Parametric sensitivity studies revealed how the fluorescence enhancement depends on the molecule’s location, distance and orientation. Coupled systems designed for simultaneous improvement exhibited the highest (intermediate directional) total fluorescence enhancement, which is accompanied by intermediate sensitivity to the molecule’s parameters, except the location and orientation sensitivity at the excitation wavelength. Gold nanorods with a geometry corresponding to the predicted optimal configurations were synthesized, and DNA strands were used to control the Cy5 dye molecule distance from the nanorod surface via hybridization of the Cy5-labelled oligonucleotide. State-of-the-art dSTORM microscopy was used to accomplish a proof-of-concept experimental demonstration of the theoretically predicted (directional) total fluorescence enhancement. The measured fluorescence enhancement was in good agreement with theoretical predictions, thus providing a complete kit to design and prepare coupled nanosystems exhibiting plasmonically enhanced fluorescence.
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spelling pubmed-73522402020-07-21 Mapping Fluorescence Enhancement of Plasmonic Nanorod Coupled Dye Molecules Tóth, Emese Ungor, Ditta Novák, Tibor Ferenc, Györgyi Bánhelyi, Balázs Csapó, Edit Erdélyi, Miklós Csete, Mária Nanomaterials (Basel) Article Plasmonically enhanced fluorescence is a widely studied and applied phenomenon, however, only a comparative theoretical and experimental analysis of coupled fluorophores and plasmonic nanoresonators makes it possible to uncover how this phenomenon can be controlled. A numerical optimization method was applied to design configurations that are capable of resulting in an enhancement of excitation and emission, moreover, of both phenomena simultaneously in coupled Cy5 dye molecule and gold nanorod systems. Parametric sensitivity studies revealed how the fluorescence enhancement depends on the molecule’s location, distance and orientation. Coupled systems designed for simultaneous improvement exhibited the highest (intermediate directional) total fluorescence enhancement, which is accompanied by intermediate sensitivity to the molecule’s parameters, except the location and orientation sensitivity at the excitation wavelength. Gold nanorods with a geometry corresponding to the predicted optimal configurations were synthesized, and DNA strands were used to control the Cy5 dye molecule distance from the nanorod surface via hybridization of the Cy5-labelled oligonucleotide. State-of-the-art dSTORM microscopy was used to accomplish a proof-of-concept experimental demonstration of the theoretically predicted (directional) total fluorescence enhancement. The measured fluorescence enhancement was in good agreement with theoretical predictions, thus providing a complete kit to design and prepare coupled nanosystems exhibiting plasmonically enhanced fluorescence. MDPI 2020-05-29 /pmc/articles/PMC7352240/ /pubmed/32485951 http://dx.doi.org/10.3390/nano10061048 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Tóth, Emese
Ungor, Ditta
Novák, Tibor
Ferenc, Györgyi
Bánhelyi, Balázs
Csapó, Edit
Erdélyi, Miklós
Csete, Mária
Mapping Fluorescence Enhancement of Plasmonic Nanorod Coupled Dye Molecules
title Mapping Fluorescence Enhancement of Plasmonic Nanorod Coupled Dye Molecules
title_full Mapping Fluorescence Enhancement of Plasmonic Nanorod Coupled Dye Molecules
title_fullStr Mapping Fluorescence Enhancement of Plasmonic Nanorod Coupled Dye Molecules
title_full_unstemmed Mapping Fluorescence Enhancement of Plasmonic Nanorod Coupled Dye Molecules
title_short Mapping Fluorescence Enhancement of Plasmonic Nanorod Coupled Dye Molecules
title_sort mapping fluorescence enhancement of plasmonic nanorod coupled dye molecules
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7352240/
https://www.ncbi.nlm.nih.gov/pubmed/32485951
http://dx.doi.org/10.3390/nano10061048
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