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Hepatocellular Carcinoma Emergence in Diabetic Mice with Non-Alcoholic Steatohepatitis Depends on Diet and Is Delayed in Liver Exhibiting an Active Immune Response

The increase of the sedentary lifestyle and high-calorie diet have modified the etiological landscape of hepatocellular carcinoma (HCC), with a recrudescence of non-alcoholic fatty liver disease (NAFLD), especially in Western countries. The purpose of our study was to evaluate the impact of high-fat...

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Autores principales: Simoes Eugénio, Mélanie, Farooq, Muhammad, Dion, Sarah, Devisme, Christelle, Raguenes-Nicol, Céline, Piquet-Pellorce, Claire, Samson, Michel, Dimanche-Boitrel, Marie-Thérèse, Le Seyec, Jacques
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7352283/
https://www.ncbi.nlm.nih.gov/pubmed/32521615
http://dx.doi.org/10.3390/cancers12061491
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author Simoes Eugénio, Mélanie
Farooq, Muhammad
Dion, Sarah
Devisme, Christelle
Raguenes-Nicol, Céline
Piquet-Pellorce, Claire
Samson, Michel
Dimanche-Boitrel, Marie-Thérèse
Le Seyec, Jacques
author_facet Simoes Eugénio, Mélanie
Farooq, Muhammad
Dion, Sarah
Devisme, Christelle
Raguenes-Nicol, Céline
Piquet-Pellorce, Claire
Samson, Michel
Dimanche-Boitrel, Marie-Thérèse
Le Seyec, Jacques
author_sort Simoes Eugénio, Mélanie
collection PubMed
description The increase of the sedentary lifestyle and high-calorie diet have modified the etiological landscape of hepatocellular carcinoma (HCC), with a recrudescence of non-alcoholic fatty liver disease (NAFLD), especially in Western countries. The purpose of our study was to evaluate the impact of high-fat diet feeding on non-alcoholic steatohepatitis (NASH) establishment and HCC development. Streptozotocin-induced diabetic male mice were fed with high-fat-high-cholesterol diet (HFHCD) or high-fat-high-sugar diet (HFHSD) from 1 to 16 weeks. Even if liver tumors appear regardless of the high-fat diet, two distinct physiopathological patterns were evidenced, with much more severe NASH hallmarks (liver injury, inflammation and fibrosis) in diabetic mice fed with HFHCD. The mild hepatic injury, weak inflammation and fibrosis observed in HFHSD were interestingly associated with earlier emergence of more numerous liver tumors. When activated helper and cytotoxic T cells, detected by flow cytometry, infiltrated the liver of HFHCD-fed diabetic mice, a delay in the appearance of tumor nodules and a limitation of their numbers were observed, suggesting that the immune activities partly controlled tumor emergence. These data highlighted two different mouse models of HCC progression in diabetic mice depending on diet, which could be useful to evaluate new therapeutic approaches for HCC by targeting the immune response.
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spelling pubmed-73522832020-07-21 Hepatocellular Carcinoma Emergence in Diabetic Mice with Non-Alcoholic Steatohepatitis Depends on Diet and Is Delayed in Liver Exhibiting an Active Immune Response Simoes Eugénio, Mélanie Farooq, Muhammad Dion, Sarah Devisme, Christelle Raguenes-Nicol, Céline Piquet-Pellorce, Claire Samson, Michel Dimanche-Boitrel, Marie-Thérèse Le Seyec, Jacques Cancers (Basel) Article The increase of the sedentary lifestyle and high-calorie diet have modified the etiological landscape of hepatocellular carcinoma (HCC), with a recrudescence of non-alcoholic fatty liver disease (NAFLD), especially in Western countries. The purpose of our study was to evaluate the impact of high-fat diet feeding on non-alcoholic steatohepatitis (NASH) establishment and HCC development. Streptozotocin-induced diabetic male mice were fed with high-fat-high-cholesterol diet (HFHCD) or high-fat-high-sugar diet (HFHSD) from 1 to 16 weeks. Even if liver tumors appear regardless of the high-fat diet, two distinct physiopathological patterns were evidenced, with much more severe NASH hallmarks (liver injury, inflammation and fibrosis) in diabetic mice fed with HFHCD. The mild hepatic injury, weak inflammation and fibrosis observed in HFHSD were interestingly associated with earlier emergence of more numerous liver tumors. When activated helper and cytotoxic T cells, detected by flow cytometry, infiltrated the liver of HFHCD-fed diabetic mice, a delay in the appearance of tumor nodules and a limitation of their numbers were observed, suggesting that the immune activities partly controlled tumor emergence. These data highlighted two different mouse models of HCC progression in diabetic mice depending on diet, which could be useful to evaluate new therapeutic approaches for HCC by targeting the immune response. MDPI 2020-06-08 /pmc/articles/PMC7352283/ /pubmed/32521615 http://dx.doi.org/10.3390/cancers12061491 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Simoes Eugénio, Mélanie
Farooq, Muhammad
Dion, Sarah
Devisme, Christelle
Raguenes-Nicol, Céline
Piquet-Pellorce, Claire
Samson, Michel
Dimanche-Boitrel, Marie-Thérèse
Le Seyec, Jacques
Hepatocellular Carcinoma Emergence in Diabetic Mice with Non-Alcoholic Steatohepatitis Depends on Diet and Is Delayed in Liver Exhibiting an Active Immune Response
title Hepatocellular Carcinoma Emergence in Diabetic Mice with Non-Alcoholic Steatohepatitis Depends on Diet and Is Delayed in Liver Exhibiting an Active Immune Response
title_full Hepatocellular Carcinoma Emergence in Diabetic Mice with Non-Alcoholic Steatohepatitis Depends on Diet and Is Delayed in Liver Exhibiting an Active Immune Response
title_fullStr Hepatocellular Carcinoma Emergence in Diabetic Mice with Non-Alcoholic Steatohepatitis Depends on Diet and Is Delayed in Liver Exhibiting an Active Immune Response
title_full_unstemmed Hepatocellular Carcinoma Emergence in Diabetic Mice with Non-Alcoholic Steatohepatitis Depends on Diet and Is Delayed in Liver Exhibiting an Active Immune Response
title_short Hepatocellular Carcinoma Emergence in Diabetic Mice with Non-Alcoholic Steatohepatitis Depends on Diet and Is Delayed in Liver Exhibiting an Active Immune Response
title_sort hepatocellular carcinoma emergence in diabetic mice with non-alcoholic steatohepatitis depends on diet and is delayed in liver exhibiting an active immune response
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7352283/
https://www.ncbi.nlm.nih.gov/pubmed/32521615
http://dx.doi.org/10.3390/cancers12061491
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