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Effect of Oocyte Quality Assessed by Brilliant Cresyl Blue (BCB) Staining on Cumulus Cell Expansion and Sonic Hedgehog Signaling in Porcine during In Vitro Maturation

Brilliant cresyl blue (BCB) staining is used to select developmentally competent cumulus-oocyte complexes (COCs) for in vitro maturation (IVM). However, limited attention has been paid to what drives the higher developmental competence of BCB+ COCs. Sonic hedgehog signaling (SHH) is an important sig...

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Autores principales: Lee, Sanghoon, Kang, Hyo-Gu, Jeong, Pil-Soo, Nanjidsuren, Tsevelmaa, Song, Bong-Seok, Jin, Yeung Bae, Lee, Sang-Rae, Kim, Sun-Uk, Sim, Bo-Woong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7352309/
https://www.ncbi.nlm.nih.gov/pubmed/32580308
http://dx.doi.org/10.3390/ijms21124423
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author Lee, Sanghoon
Kang, Hyo-Gu
Jeong, Pil-Soo
Nanjidsuren, Tsevelmaa
Song, Bong-Seok
Jin, Yeung Bae
Lee, Sang-Rae
Kim, Sun-Uk
Sim, Bo-Woong
author_facet Lee, Sanghoon
Kang, Hyo-Gu
Jeong, Pil-Soo
Nanjidsuren, Tsevelmaa
Song, Bong-Seok
Jin, Yeung Bae
Lee, Sang-Rae
Kim, Sun-Uk
Sim, Bo-Woong
author_sort Lee, Sanghoon
collection PubMed
description Brilliant cresyl blue (BCB) staining is used to select developmentally competent cumulus-oocyte complexes (COCs) for in vitro maturation (IVM). However, limited attention has been paid to what drives the higher developmental competence of BCB+ COCs. Sonic hedgehog signaling (SHH) is an important signaling pathway for ovarian follicular development and oocyte maturation. Therefore, this study investigated the effect of oocyte quality assessed by BCB staining on cumulus cell expansion, oocyte nuclear maturation, subsequent embryo development, apoptosis levels, and SHH signaling protein expression, in porcine COCs. After IVM, BCB+ COCs exhibited a significantly higher proportion of complete cumulus cell expansion and metaphase II rate in oocytes than BCB- COCs. After in vitro fertilization, the BCB+ group showed a significantly higher monospermy rate, fertilization efficiency, percentage of cleavage and blastocyst formation, with a higher total cell number and a lower apoptosis in blastocysts as compared with the BCB- group. Furthermore, significantly lower apoptosis levels and a higher expression of SHH-signaling proteins in COCs were observed, before and after IVM. In conclusion, high-quality oocytes had a greater potential to expand their surrounding cumulus cells with active SHH signaling and a lower apoptosis. This could provide COCs with a proper environment for maturation, thereby leading to a better subsequent embryo development.
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spelling pubmed-73523092020-07-21 Effect of Oocyte Quality Assessed by Brilliant Cresyl Blue (BCB) Staining on Cumulus Cell Expansion and Sonic Hedgehog Signaling in Porcine during In Vitro Maturation Lee, Sanghoon Kang, Hyo-Gu Jeong, Pil-Soo Nanjidsuren, Tsevelmaa Song, Bong-Seok Jin, Yeung Bae Lee, Sang-Rae Kim, Sun-Uk Sim, Bo-Woong Int J Mol Sci Article Brilliant cresyl blue (BCB) staining is used to select developmentally competent cumulus-oocyte complexes (COCs) for in vitro maturation (IVM). However, limited attention has been paid to what drives the higher developmental competence of BCB+ COCs. Sonic hedgehog signaling (SHH) is an important signaling pathway for ovarian follicular development and oocyte maturation. Therefore, this study investigated the effect of oocyte quality assessed by BCB staining on cumulus cell expansion, oocyte nuclear maturation, subsequent embryo development, apoptosis levels, and SHH signaling protein expression, in porcine COCs. After IVM, BCB+ COCs exhibited a significantly higher proportion of complete cumulus cell expansion and metaphase II rate in oocytes than BCB- COCs. After in vitro fertilization, the BCB+ group showed a significantly higher monospermy rate, fertilization efficiency, percentage of cleavage and blastocyst formation, with a higher total cell number and a lower apoptosis in blastocysts as compared with the BCB- group. Furthermore, significantly lower apoptosis levels and a higher expression of SHH-signaling proteins in COCs were observed, before and after IVM. In conclusion, high-quality oocytes had a greater potential to expand their surrounding cumulus cells with active SHH signaling and a lower apoptosis. This could provide COCs with a proper environment for maturation, thereby leading to a better subsequent embryo development. MDPI 2020-06-22 /pmc/articles/PMC7352309/ /pubmed/32580308 http://dx.doi.org/10.3390/ijms21124423 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Lee, Sanghoon
Kang, Hyo-Gu
Jeong, Pil-Soo
Nanjidsuren, Tsevelmaa
Song, Bong-Seok
Jin, Yeung Bae
Lee, Sang-Rae
Kim, Sun-Uk
Sim, Bo-Woong
Effect of Oocyte Quality Assessed by Brilliant Cresyl Blue (BCB) Staining on Cumulus Cell Expansion and Sonic Hedgehog Signaling in Porcine during In Vitro Maturation
title Effect of Oocyte Quality Assessed by Brilliant Cresyl Blue (BCB) Staining on Cumulus Cell Expansion and Sonic Hedgehog Signaling in Porcine during In Vitro Maturation
title_full Effect of Oocyte Quality Assessed by Brilliant Cresyl Blue (BCB) Staining on Cumulus Cell Expansion and Sonic Hedgehog Signaling in Porcine during In Vitro Maturation
title_fullStr Effect of Oocyte Quality Assessed by Brilliant Cresyl Blue (BCB) Staining on Cumulus Cell Expansion and Sonic Hedgehog Signaling in Porcine during In Vitro Maturation
title_full_unstemmed Effect of Oocyte Quality Assessed by Brilliant Cresyl Blue (BCB) Staining on Cumulus Cell Expansion and Sonic Hedgehog Signaling in Porcine during In Vitro Maturation
title_short Effect of Oocyte Quality Assessed by Brilliant Cresyl Blue (BCB) Staining on Cumulus Cell Expansion and Sonic Hedgehog Signaling in Porcine during In Vitro Maturation
title_sort effect of oocyte quality assessed by brilliant cresyl blue (bcb) staining on cumulus cell expansion and sonic hedgehog signaling in porcine during in vitro maturation
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7352309/
https://www.ncbi.nlm.nih.gov/pubmed/32580308
http://dx.doi.org/10.3390/ijms21124423
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