Cargando…

Pt Nanoparticles with High Oxidase-Like Activity and Reusability for Detection of Ascorbic Acid

Noble metal nanoenzymes such as Pt, Au, Pd, etc. exhibit magnificent activity. However, due to the scarce reserves and expensive prices of precious metals, it is essential to investigate their enzyme-like activity and explore the possibility of their reuse. In this work, the oxidase-like activity an...

Descripción completa

Detalles Bibliográficos
Autores principales: Cheng, Qin, Yang, Yong, Peng, Yusi, Liu, Meng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7352317/
https://www.ncbi.nlm.nih.gov/pubmed/32466542
http://dx.doi.org/10.3390/nano10061015
_version_ 1783557609581707264
author Cheng, Qin
Yang, Yong
Peng, Yusi
Liu, Meng
author_facet Cheng, Qin
Yang, Yong
Peng, Yusi
Liu, Meng
author_sort Cheng, Qin
collection PubMed
description Noble metal nanoenzymes such as Pt, Au, Pd, etc. exhibit magnificent activity. However, due to the scarce reserves and expensive prices of precious metals, it is essential to investigate their enzyme-like activity and explore the possibility of their reuse. In this work, the oxidase-like activity and reusability of several Pt nanoparticles with different morphologies were detected. We compared the Pt nanoparticles (NPs) with a size of about 30 nm self-assembled by 5 nm Pt nanoparticles and Pt nanoparticles (Pt-0 HCl) with a diameter of about 5 nm, and found that their Michaelis−Menten constants (Km) were close and their initial performance similar, but the Pt NPs had better reusability. This was probably attributed to the stacked structure of Pt NPs, which was conducive to the substance transport and sufficient contact. At the same time, it was found that the size, dispersion, and organic substances adsorbed on the surface of Pt nanoparticles would have a significant impact on their reusability. A colorimetric detection method was designed using the oxidase-like activity of Pt NPs to detect ascorbic acid in triplicate. The limits of detection were 131 ± 15, 144 ± 14, and 152 ± 9 nM, with little difference. This research not only showed that the morphology of the catalyst could be changed and its catalytic performance could be controlled by a simple liquid phase synthesis method, but also that it had great significance for the reuse of Pt nanoenzymes in the field of bioanalysis.
format Online
Article
Text
id pubmed-7352317
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-73523172020-07-21 Pt Nanoparticles with High Oxidase-Like Activity and Reusability for Detection of Ascorbic Acid Cheng, Qin Yang, Yong Peng, Yusi Liu, Meng Nanomaterials (Basel) Article Noble metal nanoenzymes such as Pt, Au, Pd, etc. exhibit magnificent activity. However, due to the scarce reserves and expensive prices of precious metals, it is essential to investigate their enzyme-like activity and explore the possibility of their reuse. In this work, the oxidase-like activity and reusability of several Pt nanoparticles with different morphologies were detected. We compared the Pt nanoparticles (NPs) with a size of about 30 nm self-assembled by 5 nm Pt nanoparticles and Pt nanoparticles (Pt-0 HCl) with a diameter of about 5 nm, and found that their Michaelis−Menten constants (Km) were close and their initial performance similar, but the Pt NPs had better reusability. This was probably attributed to the stacked structure of Pt NPs, which was conducive to the substance transport and sufficient contact. At the same time, it was found that the size, dispersion, and organic substances adsorbed on the surface of Pt nanoparticles would have a significant impact on their reusability. A colorimetric detection method was designed using the oxidase-like activity of Pt NPs to detect ascorbic acid in triplicate. The limits of detection were 131 ± 15, 144 ± 14, and 152 ± 9 nM, with little difference. This research not only showed that the morphology of the catalyst could be changed and its catalytic performance could be controlled by a simple liquid phase synthesis method, but also that it had great significance for the reuse of Pt nanoenzymes in the field of bioanalysis. MDPI 2020-05-26 /pmc/articles/PMC7352317/ /pubmed/32466542 http://dx.doi.org/10.3390/nano10061015 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Cheng, Qin
Yang, Yong
Peng, Yusi
Liu, Meng
Pt Nanoparticles with High Oxidase-Like Activity and Reusability for Detection of Ascorbic Acid
title Pt Nanoparticles with High Oxidase-Like Activity and Reusability for Detection of Ascorbic Acid
title_full Pt Nanoparticles with High Oxidase-Like Activity and Reusability for Detection of Ascorbic Acid
title_fullStr Pt Nanoparticles with High Oxidase-Like Activity and Reusability for Detection of Ascorbic Acid
title_full_unstemmed Pt Nanoparticles with High Oxidase-Like Activity and Reusability for Detection of Ascorbic Acid
title_short Pt Nanoparticles with High Oxidase-Like Activity and Reusability for Detection of Ascorbic Acid
title_sort pt nanoparticles with high oxidase-like activity and reusability for detection of ascorbic acid
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7352317/
https://www.ncbi.nlm.nih.gov/pubmed/32466542
http://dx.doi.org/10.3390/nano10061015
work_keys_str_mv AT chengqin ptnanoparticleswithhighoxidaselikeactivityandreusabilityfordetectionofascorbicacid
AT yangyong ptnanoparticleswithhighoxidaselikeactivityandreusabilityfordetectionofascorbicacid
AT pengyusi ptnanoparticleswithhighoxidaselikeactivityandreusabilityfordetectionofascorbicacid
AT liumeng ptnanoparticleswithhighoxidaselikeactivityandreusabilityfordetectionofascorbicacid