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Applying Tissue Slice Culture in Cancer Research—Insights from Preclinical Proton Radiotherapy

A challenge in cancer research is the definition of reproducible, reliable, and practical models, which reflect the effects of complex treatment modalities and the heterogeneous response of patients. Proton beam radiotherapy (PBRT), relative to conventional photon-based radiotherapy, offers the pote...

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Autores principales: Suckert, Theresa, Rassamegevanon, Treewut, Müller, Johannes, Dietrich, Antje, Graja, Antonia, Reiche, Michael, Löck, Steffen, Krause, Mechthild, Beyreuther, Elke, von Neubeck, Cläre
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7352770/
https://www.ncbi.nlm.nih.gov/pubmed/32560230
http://dx.doi.org/10.3390/cancers12061589
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author Suckert, Theresa
Rassamegevanon, Treewut
Müller, Johannes
Dietrich, Antje
Graja, Antonia
Reiche, Michael
Löck, Steffen
Krause, Mechthild
Beyreuther, Elke
von Neubeck, Cläre
author_facet Suckert, Theresa
Rassamegevanon, Treewut
Müller, Johannes
Dietrich, Antje
Graja, Antonia
Reiche, Michael
Löck, Steffen
Krause, Mechthild
Beyreuther, Elke
von Neubeck, Cläre
author_sort Suckert, Theresa
collection PubMed
description A challenge in cancer research is the definition of reproducible, reliable, and practical models, which reflect the effects of complex treatment modalities and the heterogeneous response of patients. Proton beam radiotherapy (PBRT), relative to conventional photon-based radiotherapy, offers the potential for iso-effective tumor control, while protecting the normal tissue surrounding the tumor. However, the effects of PBRT on the tumor microenvironment and the interplay with newly developed chemo- and immunotherapeutic approaches are still open for investigation. This work evaluated thin-cut tumor slice cultures (TSC) of head and neck cancer and organotypic brain slice cultures (OBSC) of adult mice brain, regarding their relevance for translational radiooncology research. TSC and OBSC were treated with PBRT and investigated for cell survival with a lactate dehydrogenase (LDH) assay, DNA repair via the DNA double strand break marker γH2AX, as well as histology with regards to morphology. Adult OBSC failed to be an appropriate model for radiobiological research questions. However, histological analysis of TSC showed DNA damage and tumor morphological results, comparable to known in vivo and in vitro data, making them a promising model to study novel treatment approaches in patient-derived xenografts or primary tumor material.
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spelling pubmed-73527702020-07-15 Applying Tissue Slice Culture in Cancer Research—Insights from Preclinical Proton Radiotherapy Suckert, Theresa Rassamegevanon, Treewut Müller, Johannes Dietrich, Antje Graja, Antonia Reiche, Michael Löck, Steffen Krause, Mechthild Beyreuther, Elke von Neubeck, Cläre Cancers (Basel) Article A challenge in cancer research is the definition of reproducible, reliable, and practical models, which reflect the effects of complex treatment modalities and the heterogeneous response of patients. Proton beam radiotherapy (PBRT), relative to conventional photon-based radiotherapy, offers the potential for iso-effective tumor control, while protecting the normal tissue surrounding the tumor. However, the effects of PBRT on the tumor microenvironment and the interplay with newly developed chemo- and immunotherapeutic approaches are still open for investigation. This work evaluated thin-cut tumor slice cultures (TSC) of head and neck cancer and organotypic brain slice cultures (OBSC) of adult mice brain, regarding their relevance for translational radiooncology research. TSC and OBSC were treated with PBRT and investigated for cell survival with a lactate dehydrogenase (LDH) assay, DNA repair via the DNA double strand break marker γH2AX, as well as histology with regards to morphology. Adult OBSC failed to be an appropriate model for radiobiological research questions. However, histological analysis of TSC showed DNA damage and tumor morphological results, comparable to known in vivo and in vitro data, making them a promising model to study novel treatment approaches in patient-derived xenografts or primary tumor material. MDPI 2020-06-16 /pmc/articles/PMC7352770/ /pubmed/32560230 http://dx.doi.org/10.3390/cancers12061589 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Suckert, Theresa
Rassamegevanon, Treewut
Müller, Johannes
Dietrich, Antje
Graja, Antonia
Reiche, Michael
Löck, Steffen
Krause, Mechthild
Beyreuther, Elke
von Neubeck, Cläre
Applying Tissue Slice Culture in Cancer Research—Insights from Preclinical Proton Radiotherapy
title Applying Tissue Slice Culture in Cancer Research—Insights from Preclinical Proton Radiotherapy
title_full Applying Tissue Slice Culture in Cancer Research—Insights from Preclinical Proton Radiotherapy
title_fullStr Applying Tissue Slice Culture in Cancer Research—Insights from Preclinical Proton Radiotherapy
title_full_unstemmed Applying Tissue Slice Culture in Cancer Research—Insights from Preclinical Proton Radiotherapy
title_short Applying Tissue Slice Culture in Cancer Research—Insights from Preclinical Proton Radiotherapy
title_sort applying tissue slice culture in cancer research—insights from preclinical proton radiotherapy
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7352770/
https://www.ncbi.nlm.nih.gov/pubmed/32560230
http://dx.doi.org/10.3390/cancers12061589
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