Cargando…

Decreased Excretion of Urinary Exosomal Aquaporin-2 in a Puromycin Aminonucleoside-Induced Nephrotic Syndrome Model

Urinary exosomes, small extracellular vesicles present in urine, are secreted from all types of renal epithelial cells. Aquaporin-2 (AQP2), a vasopressin-regulated water channel protein, is known to be selectively excreted into the urine through exosomes (UE-AQP2), and its renal expression is decrea...

Descripción completa

Detalles Bibliográficos
Autores principales: Abdeen, Ahmed, Sonoda, Hiroko, Kaito, Ayaha, Oshikawa-Hori, Sayaka, Fujimoto, Naruki, Ikeda, Masahiro
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7352848/
https://www.ncbi.nlm.nih.gov/pubmed/32560242
http://dx.doi.org/10.3390/ijms21124288
_version_ 1783557734586646528
author Abdeen, Ahmed
Sonoda, Hiroko
Kaito, Ayaha
Oshikawa-Hori, Sayaka
Fujimoto, Naruki
Ikeda, Masahiro
author_facet Abdeen, Ahmed
Sonoda, Hiroko
Kaito, Ayaha
Oshikawa-Hori, Sayaka
Fujimoto, Naruki
Ikeda, Masahiro
author_sort Abdeen, Ahmed
collection PubMed
description Urinary exosomes, small extracellular vesicles present in urine, are secreted from all types of renal epithelial cells. Aquaporin-2 (AQP2), a vasopressin-regulated water channel protein, is known to be selectively excreted into the urine through exosomes (UE-AQP2), and its renal expression is decreased in nephrotic syndrome. However, it is still unclear whether excretion of UE-AQP2 is altered in nephrotic syndrome. In this study, we examined the excretion of UE-AQP2 in an experimental rat model of nephrotic syndrome induced by the administration of puromycin aminonucleoside (PAN). Rats were assigned to two groups: a control group administered saline and a PAN group given a single intraperitoneal injection of PAN (125 mg/kg) at day 0. The experiment was continued for 8 days, and samples of urine, blood, and tissue were collected on days 2, 5, and 8. The blood and urine parameters revealed that PAN induced nephrotic syndrome on days 5 and 8, and decreases in the excretion of UE-AQP2 were detected on days 2 through 8 in the PAN group. Immunohistochemistry showed that the renal expression of AQP2 was decreased on days 5 and 8. The release of exosomal marker proteins into the urine through UEs was decreased on day 5 and increased on day 8. These data suggest that UE-AQP2 is decreased in PAN-induced nephrotic syndrome and that this reflects its renal expression in the marked proteinuria phase after PAN treatment.
format Online
Article
Text
id pubmed-7352848
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-73528482020-07-15 Decreased Excretion of Urinary Exosomal Aquaporin-2 in a Puromycin Aminonucleoside-Induced Nephrotic Syndrome Model Abdeen, Ahmed Sonoda, Hiroko Kaito, Ayaha Oshikawa-Hori, Sayaka Fujimoto, Naruki Ikeda, Masahiro Int J Mol Sci Article Urinary exosomes, small extracellular vesicles present in urine, are secreted from all types of renal epithelial cells. Aquaporin-2 (AQP2), a vasopressin-regulated water channel protein, is known to be selectively excreted into the urine through exosomes (UE-AQP2), and its renal expression is decreased in nephrotic syndrome. However, it is still unclear whether excretion of UE-AQP2 is altered in nephrotic syndrome. In this study, we examined the excretion of UE-AQP2 in an experimental rat model of nephrotic syndrome induced by the administration of puromycin aminonucleoside (PAN). Rats were assigned to two groups: a control group administered saline and a PAN group given a single intraperitoneal injection of PAN (125 mg/kg) at day 0. The experiment was continued for 8 days, and samples of urine, blood, and tissue were collected on days 2, 5, and 8. The blood and urine parameters revealed that PAN induced nephrotic syndrome on days 5 and 8, and decreases in the excretion of UE-AQP2 were detected on days 2 through 8 in the PAN group. Immunohistochemistry showed that the renal expression of AQP2 was decreased on days 5 and 8. The release of exosomal marker proteins into the urine through UEs was decreased on day 5 and increased on day 8. These data suggest that UE-AQP2 is decreased in PAN-induced nephrotic syndrome and that this reflects its renal expression in the marked proteinuria phase after PAN treatment. MDPI 2020-06-16 /pmc/articles/PMC7352848/ /pubmed/32560242 http://dx.doi.org/10.3390/ijms21124288 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Abdeen, Ahmed
Sonoda, Hiroko
Kaito, Ayaha
Oshikawa-Hori, Sayaka
Fujimoto, Naruki
Ikeda, Masahiro
Decreased Excretion of Urinary Exosomal Aquaporin-2 in a Puromycin Aminonucleoside-Induced Nephrotic Syndrome Model
title Decreased Excretion of Urinary Exosomal Aquaporin-2 in a Puromycin Aminonucleoside-Induced Nephrotic Syndrome Model
title_full Decreased Excretion of Urinary Exosomal Aquaporin-2 in a Puromycin Aminonucleoside-Induced Nephrotic Syndrome Model
title_fullStr Decreased Excretion of Urinary Exosomal Aquaporin-2 in a Puromycin Aminonucleoside-Induced Nephrotic Syndrome Model
title_full_unstemmed Decreased Excretion of Urinary Exosomal Aquaporin-2 in a Puromycin Aminonucleoside-Induced Nephrotic Syndrome Model
title_short Decreased Excretion of Urinary Exosomal Aquaporin-2 in a Puromycin Aminonucleoside-Induced Nephrotic Syndrome Model
title_sort decreased excretion of urinary exosomal aquaporin-2 in a puromycin aminonucleoside-induced nephrotic syndrome model
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7352848/
https://www.ncbi.nlm.nih.gov/pubmed/32560242
http://dx.doi.org/10.3390/ijms21124288
work_keys_str_mv AT abdeenahmed decreasedexcretionofurinaryexosomalaquaporin2inapuromycinaminonucleosideinducednephroticsyndromemodel
AT sonodahiroko decreasedexcretionofurinaryexosomalaquaporin2inapuromycinaminonucleosideinducednephroticsyndromemodel
AT kaitoayaha decreasedexcretionofurinaryexosomalaquaporin2inapuromycinaminonucleosideinducednephroticsyndromemodel
AT oshikawahorisayaka decreasedexcretionofurinaryexosomalaquaporin2inapuromycinaminonucleosideinducednephroticsyndromemodel
AT fujimotonaruki decreasedexcretionofurinaryexosomalaquaporin2inapuromycinaminonucleosideinducednephroticsyndromemodel
AT ikedamasahiro decreasedexcretionofurinaryexosomalaquaporin2inapuromycinaminonucleosideinducednephroticsyndromemodel