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Solid Plasmonic Substrates for Breast Cancer Detection by Means of SERS Analysis of Blood Plasma
Surface enhanced Raman spectroscopy (SERS) represents a promising technique in providing specific molecular information that could have a major impact in biomedical applications, such as early cancer detection. SERS requires the presence of a suitable plasmonic substrate that can generate enhanced a...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7353077/ https://www.ncbi.nlm.nih.gov/pubmed/32575924 http://dx.doi.org/10.3390/nano10061212 |
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author | Știufiuc, Gabriela Fabiola Toma, Valentin Buse, Mihail Mărginean, Radu Morar-Bolba, Gabriela Culic, Bogdan Tetean, Romulus Leopold, Nicolae Pavel, Ioana Lucaciu, Constantin Mihai Știufiuc, Rareș Ionuț |
author_facet | Știufiuc, Gabriela Fabiola Toma, Valentin Buse, Mihail Mărginean, Radu Morar-Bolba, Gabriela Culic, Bogdan Tetean, Romulus Leopold, Nicolae Pavel, Ioana Lucaciu, Constantin Mihai Știufiuc, Rareș Ionuț |
author_sort | Știufiuc, Gabriela Fabiola |
collection | PubMed |
description | Surface enhanced Raman spectroscopy (SERS) represents a promising technique in providing specific molecular information that could have a major impact in biomedical applications, such as early cancer detection. SERS requires the presence of a suitable plasmonic substrate that can generate enhanced and reproducible diagnostic relevant spectra. In this paper, we propose a new approach for the synthesis of such a substrate, by using concentrated silver nanoparticles purified using the Tangential Flow Filtration method. The capacity of our substrates to generate reproducible and enhanced Raman signals, in a manner that can allow cancer detection by means of Multivariate Analysis (MVA) of Surface Enhanced Raman (SER) spectra, has been tested on blood plasma samples collected from 35 healthy donors and 29 breast cancer patients. All the spectra were analyzed by a combined Principal Component-Linear Discriminant Analysis. Our results facilitated the discrimination between healthy donors and breast cancer patients with 90% sensitivity, 89% specificity and 89% accuracy. This is a direct consequence of substrates’ ability to generate diagnostic relevant spectral information by performing SERS measurements on pristine blood plasma samples. Our results suggest that this type of solid substrate could be employed for the detection of other types of cancer or other diseases by means of MVA-SERS procedure. |
format | Online Article Text |
id | pubmed-7353077 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-73530772020-07-15 Solid Plasmonic Substrates for Breast Cancer Detection by Means of SERS Analysis of Blood Plasma Știufiuc, Gabriela Fabiola Toma, Valentin Buse, Mihail Mărginean, Radu Morar-Bolba, Gabriela Culic, Bogdan Tetean, Romulus Leopold, Nicolae Pavel, Ioana Lucaciu, Constantin Mihai Știufiuc, Rareș Ionuț Nanomaterials (Basel) Article Surface enhanced Raman spectroscopy (SERS) represents a promising technique in providing specific molecular information that could have a major impact in biomedical applications, such as early cancer detection. SERS requires the presence of a suitable plasmonic substrate that can generate enhanced and reproducible diagnostic relevant spectra. In this paper, we propose a new approach for the synthesis of such a substrate, by using concentrated silver nanoparticles purified using the Tangential Flow Filtration method. The capacity of our substrates to generate reproducible and enhanced Raman signals, in a manner that can allow cancer detection by means of Multivariate Analysis (MVA) of Surface Enhanced Raman (SER) spectra, has been tested on blood plasma samples collected from 35 healthy donors and 29 breast cancer patients. All the spectra were analyzed by a combined Principal Component-Linear Discriminant Analysis. Our results facilitated the discrimination between healthy donors and breast cancer patients with 90% sensitivity, 89% specificity and 89% accuracy. This is a direct consequence of substrates’ ability to generate diagnostic relevant spectral information by performing SERS measurements on pristine blood plasma samples. Our results suggest that this type of solid substrate could be employed for the detection of other types of cancer or other diseases by means of MVA-SERS procedure. MDPI 2020-06-21 /pmc/articles/PMC7353077/ /pubmed/32575924 http://dx.doi.org/10.3390/nano10061212 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Știufiuc, Gabriela Fabiola Toma, Valentin Buse, Mihail Mărginean, Radu Morar-Bolba, Gabriela Culic, Bogdan Tetean, Romulus Leopold, Nicolae Pavel, Ioana Lucaciu, Constantin Mihai Știufiuc, Rareș Ionuț Solid Plasmonic Substrates for Breast Cancer Detection by Means of SERS Analysis of Blood Plasma |
title | Solid Plasmonic Substrates for Breast Cancer Detection by Means of SERS Analysis of Blood Plasma |
title_full | Solid Plasmonic Substrates for Breast Cancer Detection by Means of SERS Analysis of Blood Plasma |
title_fullStr | Solid Plasmonic Substrates for Breast Cancer Detection by Means of SERS Analysis of Blood Plasma |
title_full_unstemmed | Solid Plasmonic Substrates for Breast Cancer Detection by Means of SERS Analysis of Blood Plasma |
title_short | Solid Plasmonic Substrates for Breast Cancer Detection by Means of SERS Analysis of Blood Plasma |
title_sort | solid plasmonic substrates for breast cancer detection by means of sers analysis of blood plasma |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7353077/ https://www.ncbi.nlm.nih.gov/pubmed/32575924 http://dx.doi.org/10.3390/nano10061212 |
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