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Fast and Accurate Pneumocystis Pneumonia Diagnosis in Human Samples Using a Label-Free Plasmonic Biosensor

Pneumocystis jirovecii is a fungus responsible for human Pneumocystis pneumonia, one of the most severe infections encountered in immunodepressed individuals. The diagnosis of Pneumocystis pneumonia continues to be challenging due to the absence of specific symptoms in infected patients. Moreover, t...

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Autores principales: Calvo-Lozano, Olalla, Aviñó, Anna, Friaza, Vicente, Medina-Escuela, Alfonso, S. Huertas, César, Calderón, Enrique J., Eritja, Ramón, Lechuga, Laura M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7353103/
https://www.ncbi.nlm.nih.gov/pubmed/32604931
http://dx.doi.org/10.3390/nano10061246
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author Calvo-Lozano, Olalla
Aviñó, Anna
Friaza, Vicente
Medina-Escuela, Alfonso
S. Huertas, César
Calderón, Enrique J.
Eritja, Ramón
Lechuga, Laura M.
author_facet Calvo-Lozano, Olalla
Aviñó, Anna
Friaza, Vicente
Medina-Escuela, Alfonso
S. Huertas, César
Calderón, Enrique J.
Eritja, Ramón
Lechuga, Laura M.
author_sort Calvo-Lozano, Olalla
collection PubMed
description Pneumocystis jirovecii is a fungus responsible for human Pneumocystis pneumonia, one of the most severe infections encountered in immunodepressed individuals. The diagnosis of Pneumocystis pneumonia continues to be challenging due to the absence of specific symptoms in infected patients. Moreover, the standard diagnostic method employed for its diagnosis involves mainly PCR-based techniques, which besides being highly specific and sensitive, require specialized personnel and equipment and are time-consuming. Our aim is to demonstrate an optical biosensor methodology based on surface plasmon resonance to perform such diagnostics in an efficient and decentralized scheme. The biosensor methodology employs poly-purine reverse-Hoogsteen hairpin probes for the detection of the mitochondrial large subunit ribosomal RNA (mtLSU rRNA) gene, related to P. jirovecii detection. The biosensor device performs a real-time and label-free identification of the mtLSU rRNA gene with excellent selectivity and reproducibility, achieving limits of detection of around 2.11 nM. A preliminary evaluation of clinical samples showed rapid, label-free and specific identification of P. jirovecii in human lung fluids such as bronchoalveolar lavages or nasopharyngeal aspirates. These results offer a door for the future deployment of a sensitive diagnostic tool for fast, direct and selective detection of Pneumocystis pneumonia disease.
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spelling pubmed-73531032020-07-15 Fast and Accurate Pneumocystis Pneumonia Diagnosis in Human Samples Using a Label-Free Plasmonic Biosensor Calvo-Lozano, Olalla Aviñó, Anna Friaza, Vicente Medina-Escuela, Alfonso S. Huertas, César Calderón, Enrique J. Eritja, Ramón Lechuga, Laura M. Nanomaterials (Basel) Article Pneumocystis jirovecii is a fungus responsible for human Pneumocystis pneumonia, one of the most severe infections encountered in immunodepressed individuals. The diagnosis of Pneumocystis pneumonia continues to be challenging due to the absence of specific symptoms in infected patients. Moreover, the standard diagnostic method employed for its diagnosis involves mainly PCR-based techniques, which besides being highly specific and sensitive, require specialized personnel and equipment and are time-consuming. Our aim is to demonstrate an optical biosensor methodology based on surface plasmon resonance to perform such diagnostics in an efficient and decentralized scheme. The biosensor methodology employs poly-purine reverse-Hoogsteen hairpin probes for the detection of the mitochondrial large subunit ribosomal RNA (mtLSU rRNA) gene, related to P. jirovecii detection. The biosensor device performs a real-time and label-free identification of the mtLSU rRNA gene with excellent selectivity and reproducibility, achieving limits of detection of around 2.11 nM. A preliminary evaluation of clinical samples showed rapid, label-free and specific identification of P. jirovecii in human lung fluids such as bronchoalveolar lavages or nasopharyngeal aspirates. These results offer a door for the future deployment of a sensitive diagnostic tool for fast, direct and selective detection of Pneumocystis pneumonia disease. MDPI 2020-06-26 /pmc/articles/PMC7353103/ /pubmed/32604931 http://dx.doi.org/10.3390/nano10061246 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Calvo-Lozano, Olalla
Aviñó, Anna
Friaza, Vicente
Medina-Escuela, Alfonso
S. Huertas, César
Calderón, Enrique J.
Eritja, Ramón
Lechuga, Laura M.
Fast and Accurate Pneumocystis Pneumonia Diagnosis in Human Samples Using a Label-Free Plasmonic Biosensor
title Fast and Accurate Pneumocystis Pneumonia Diagnosis in Human Samples Using a Label-Free Plasmonic Biosensor
title_full Fast and Accurate Pneumocystis Pneumonia Diagnosis in Human Samples Using a Label-Free Plasmonic Biosensor
title_fullStr Fast and Accurate Pneumocystis Pneumonia Diagnosis in Human Samples Using a Label-Free Plasmonic Biosensor
title_full_unstemmed Fast and Accurate Pneumocystis Pneumonia Diagnosis in Human Samples Using a Label-Free Plasmonic Biosensor
title_short Fast and Accurate Pneumocystis Pneumonia Diagnosis in Human Samples Using a Label-Free Plasmonic Biosensor
title_sort fast and accurate pneumocystis pneumonia diagnosis in human samples using a label-free plasmonic biosensor
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7353103/
https://www.ncbi.nlm.nih.gov/pubmed/32604931
http://dx.doi.org/10.3390/nano10061246
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