Cargando…

Modulation of the Systemic Immune Response in Suckling Rats by Breast Milk TGF-β2, EGF and FGF21 Supplementation

Breast milk is a rich fluid containing bioactive compounds such as specific growth factors (GF) that contribute to maturation of the immune system in early life. The aim of this study was to determine whether transforming growth factor-β2 (TGF-β2), epidermal growth factor (EGF) and fibroblast growth...

Descripción completa

Detalles Bibliográficos
Autores principales: Torres-Castro, Paulina, Grases-Pintó, Blanca, Abril-Gil, Mar, Castell, Margarida, Rodríguez-Lagunas, María J., Pérez-Cano, Francisco J., Franch, Àngels
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7353385/
https://www.ncbi.nlm.nih.gov/pubmed/32599899
http://dx.doi.org/10.3390/nu12061888
_version_ 1783557863404208128
author Torres-Castro, Paulina
Grases-Pintó, Blanca
Abril-Gil, Mar
Castell, Margarida
Rodríguez-Lagunas, María J.
Pérez-Cano, Francisco J.
Franch, Àngels
author_facet Torres-Castro, Paulina
Grases-Pintó, Blanca
Abril-Gil, Mar
Castell, Margarida
Rodríguez-Lagunas, María J.
Pérez-Cano, Francisco J.
Franch, Àngels
author_sort Torres-Castro, Paulina
collection PubMed
description Breast milk is a rich fluid containing bioactive compounds such as specific growth factors (GF) that contribute to maturation of the immune system in early life. The aim of this study was to determine whether transforming growth factor-β2 (TGF-β2), epidermal growth factor (EGF) and fibroblast growth factor 21 (FGF21), compounds present in breast milk, could promote systemic immune maturation. For this purpose, newborn Wistar rats were daily supplemented with these GF by oral gavage during the suckling period (21 days of life). At day 14 and 21 of life, plasma for immunoglobulin (Ig) quantification was obtained and spleen lymphocytes were isolated, immunophenotyped and cultured to evaluate their ability to proliferate and release cytokines. The main result was obtained at day 14, when supplementation with EGF increased B cell proportion to reach levels observed at day 21. At the end of the suckling period, all GF increased the plasma levels of IgG1 and IgG2a isotypes, FGF21 balanced the Th1/Th2 cytokine response and both EGF and FGF21 modified splenic lymphocyte composition. These results suggested that the studied milk bioactive factors, mainly EGF and FGF21, may have modulatory roles in the systemic immune responses in early life, although their physiological roles remain to be established.
format Online
Article
Text
id pubmed-7353385
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-73533852020-07-15 Modulation of the Systemic Immune Response in Suckling Rats by Breast Milk TGF-β2, EGF and FGF21 Supplementation Torres-Castro, Paulina Grases-Pintó, Blanca Abril-Gil, Mar Castell, Margarida Rodríguez-Lagunas, María J. Pérez-Cano, Francisco J. Franch, Àngels Nutrients Article Breast milk is a rich fluid containing bioactive compounds such as specific growth factors (GF) that contribute to maturation of the immune system in early life. The aim of this study was to determine whether transforming growth factor-β2 (TGF-β2), epidermal growth factor (EGF) and fibroblast growth factor 21 (FGF21), compounds present in breast milk, could promote systemic immune maturation. For this purpose, newborn Wistar rats were daily supplemented with these GF by oral gavage during the suckling period (21 days of life). At day 14 and 21 of life, plasma for immunoglobulin (Ig) quantification was obtained and spleen lymphocytes were isolated, immunophenotyped and cultured to evaluate their ability to proliferate and release cytokines. The main result was obtained at day 14, when supplementation with EGF increased B cell proportion to reach levels observed at day 21. At the end of the suckling period, all GF increased the plasma levels of IgG1 and IgG2a isotypes, FGF21 balanced the Th1/Th2 cytokine response and both EGF and FGF21 modified splenic lymphocyte composition. These results suggested that the studied milk bioactive factors, mainly EGF and FGF21, may have modulatory roles in the systemic immune responses in early life, although their physiological roles remain to be established. MDPI 2020-06-24 /pmc/articles/PMC7353385/ /pubmed/32599899 http://dx.doi.org/10.3390/nu12061888 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Torres-Castro, Paulina
Grases-Pintó, Blanca
Abril-Gil, Mar
Castell, Margarida
Rodríguez-Lagunas, María J.
Pérez-Cano, Francisco J.
Franch, Àngels
Modulation of the Systemic Immune Response in Suckling Rats by Breast Milk TGF-β2, EGF and FGF21 Supplementation
title Modulation of the Systemic Immune Response in Suckling Rats by Breast Milk TGF-β2, EGF and FGF21 Supplementation
title_full Modulation of the Systemic Immune Response in Suckling Rats by Breast Milk TGF-β2, EGF and FGF21 Supplementation
title_fullStr Modulation of the Systemic Immune Response in Suckling Rats by Breast Milk TGF-β2, EGF and FGF21 Supplementation
title_full_unstemmed Modulation of the Systemic Immune Response in Suckling Rats by Breast Milk TGF-β2, EGF and FGF21 Supplementation
title_short Modulation of the Systemic Immune Response in Suckling Rats by Breast Milk TGF-β2, EGF and FGF21 Supplementation
title_sort modulation of the systemic immune response in suckling rats by breast milk tgf-β2, egf and fgf21 supplementation
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7353385/
https://www.ncbi.nlm.nih.gov/pubmed/32599899
http://dx.doi.org/10.3390/nu12061888
work_keys_str_mv AT torrescastropaulina modulationofthesystemicimmuneresponseinsucklingratsbybreastmilktgfb2egfandfgf21supplementation
AT grasespintoblanca modulationofthesystemicimmuneresponseinsucklingratsbybreastmilktgfb2egfandfgf21supplementation
AT abrilgilmar modulationofthesystemicimmuneresponseinsucklingratsbybreastmilktgfb2egfandfgf21supplementation
AT castellmargarida modulationofthesystemicimmuneresponseinsucklingratsbybreastmilktgfb2egfandfgf21supplementation
AT rodriguezlagunasmariaj modulationofthesystemicimmuneresponseinsucklingratsbybreastmilktgfb2egfandfgf21supplementation
AT perezcanofranciscoj modulationofthesystemicimmuneresponseinsucklingratsbybreastmilktgfb2egfandfgf21supplementation
AT franchangels modulationofthesystemicimmuneresponseinsucklingratsbybreastmilktgfb2egfandfgf21supplementation