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Gold Nanoparticle-Assisted Virus Formation by Means of the Delivery of an Oncolytic Adenovirus Genome
Oncolytic adenoviruses are a therapeutic alternative to treat cancer based on their ability to replicate selectively in tumor cells. However, their use is limited mainly by the neutralizing antibody (Nab) immune response that prevents repeated dosing. An alternative to facilitate the DNA access to t...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7353451/ https://www.ncbi.nlm.nih.gov/pubmed/32560474 http://dx.doi.org/10.3390/nano10061183 |
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author | Sendra, Luis Miguel, Antonio Navarro-Plaza, M. Carmen Herrero, María José de la Higuera, José Cháfer-Pericás, Consuelo Aznar, Elena Marcos, M. Dolores Martínez-Máñez, Ramón Rojas, Luis Alfonso Alemany, Ramón Aliño, Salvador F. |
author_facet | Sendra, Luis Miguel, Antonio Navarro-Plaza, M. Carmen Herrero, María José de la Higuera, José Cháfer-Pericás, Consuelo Aznar, Elena Marcos, M. Dolores Martínez-Máñez, Ramón Rojas, Luis Alfonso Alemany, Ramón Aliño, Salvador F. |
author_sort | Sendra, Luis |
collection | PubMed |
description | Oncolytic adenoviruses are a therapeutic alternative to treat cancer based on their ability to replicate selectively in tumor cells. However, their use is limited mainly by the neutralizing antibody (Nab) immune response that prevents repeated dosing. An alternative to facilitate the DNA access to the tumor even in the presence of anti-viral Nabs could be gold nanoparticles able to transfer DNA molecules. However, the ability of these nanoparticles to carry large DNA molecules, such as an oncolytic adenovirus genome, has not been studied. In this work, gold nanoparticles were functionalized with different amounts of polyethylenimine to transfer in a safe and efficient manner a large oncolytic virus genome. Their transfer efficacy and final effect of the oncolytic virus in cancer cells are studied. For each synthesized nanoparticle, (a) DNA loading capacity, (b) complex size, (c) DNA protection ability, (d) transfection efficacy and (e) cytotoxic effect were studied. We observed that small gold nanoparticles (70–80 nm in diameter) protected DNA against nucleases and were able to transfect the ICOVIR-15 oncolytic virus genome encoded in pLR1 plasmid. In the present work, efficient transgene RNA expression, luciferase activity and viral cytopathic effect on cancer cells are reported. These results suggest gold nanoparticles to be an efficient and safe vector for oncolytic adenovirus genome transfer. |
format | Online Article Text |
id | pubmed-7353451 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-73534512020-07-15 Gold Nanoparticle-Assisted Virus Formation by Means of the Delivery of an Oncolytic Adenovirus Genome Sendra, Luis Miguel, Antonio Navarro-Plaza, M. Carmen Herrero, María José de la Higuera, José Cháfer-Pericás, Consuelo Aznar, Elena Marcos, M. Dolores Martínez-Máñez, Ramón Rojas, Luis Alfonso Alemany, Ramón Aliño, Salvador F. Nanomaterials (Basel) Article Oncolytic adenoviruses are a therapeutic alternative to treat cancer based on their ability to replicate selectively in tumor cells. However, their use is limited mainly by the neutralizing antibody (Nab) immune response that prevents repeated dosing. An alternative to facilitate the DNA access to the tumor even in the presence of anti-viral Nabs could be gold nanoparticles able to transfer DNA molecules. However, the ability of these nanoparticles to carry large DNA molecules, such as an oncolytic adenovirus genome, has not been studied. In this work, gold nanoparticles were functionalized with different amounts of polyethylenimine to transfer in a safe and efficient manner a large oncolytic virus genome. Their transfer efficacy and final effect of the oncolytic virus in cancer cells are studied. For each synthesized nanoparticle, (a) DNA loading capacity, (b) complex size, (c) DNA protection ability, (d) transfection efficacy and (e) cytotoxic effect were studied. We observed that small gold nanoparticles (70–80 nm in diameter) protected DNA against nucleases and were able to transfect the ICOVIR-15 oncolytic virus genome encoded in pLR1 plasmid. In the present work, efficient transgene RNA expression, luciferase activity and viral cytopathic effect on cancer cells are reported. These results suggest gold nanoparticles to be an efficient and safe vector for oncolytic adenovirus genome transfer. MDPI 2020-06-17 /pmc/articles/PMC7353451/ /pubmed/32560474 http://dx.doi.org/10.3390/nano10061183 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Sendra, Luis Miguel, Antonio Navarro-Plaza, M. Carmen Herrero, María José de la Higuera, José Cháfer-Pericás, Consuelo Aznar, Elena Marcos, M. Dolores Martínez-Máñez, Ramón Rojas, Luis Alfonso Alemany, Ramón Aliño, Salvador F. Gold Nanoparticle-Assisted Virus Formation by Means of the Delivery of an Oncolytic Adenovirus Genome |
title | Gold Nanoparticle-Assisted Virus Formation by Means of the Delivery of an Oncolytic Adenovirus Genome |
title_full | Gold Nanoparticle-Assisted Virus Formation by Means of the Delivery of an Oncolytic Adenovirus Genome |
title_fullStr | Gold Nanoparticle-Assisted Virus Formation by Means of the Delivery of an Oncolytic Adenovirus Genome |
title_full_unstemmed | Gold Nanoparticle-Assisted Virus Formation by Means of the Delivery of an Oncolytic Adenovirus Genome |
title_short | Gold Nanoparticle-Assisted Virus Formation by Means of the Delivery of an Oncolytic Adenovirus Genome |
title_sort | gold nanoparticle-assisted virus formation by means of the delivery of an oncolytic adenovirus genome |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7353451/ https://www.ncbi.nlm.nih.gov/pubmed/32560474 http://dx.doi.org/10.3390/nano10061183 |
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