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Acute Oral Toxicity and Genotoxicity of Polysaccharide Fraction from Young Barley Leaves (Hordeum vulgare L.)

Polysaccharides isolated from various plants are considered precious bioactive materials owing to their potent biological activities. Previously, we prepared a polysaccharide fraction (BLE0) isolated from young barley leaves (Hordeum vulgare L.), demonstrating its anti-osteoporotic and immunostimula...

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Autores principales: Cho, Chang-Won, Song, Young-Ran, Lim, Won-Chul, Hwang, Youn-Hwan, Rhee, Young Kyoung, Choi, Jae Woong, Lee, Kyung-Tae, Hong, Hee-Do
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7353472/
https://www.ncbi.nlm.nih.gov/pubmed/32575580
http://dx.doi.org/10.3390/foods9060809
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author Cho, Chang-Won
Song, Young-Ran
Lim, Won-Chul
Hwang, Youn-Hwan
Rhee, Young Kyoung
Choi, Jae Woong
Lee, Kyung-Tae
Hong, Hee-Do
author_facet Cho, Chang-Won
Song, Young-Ran
Lim, Won-Chul
Hwang, Youn-Hwan
Rhee, Young Kyoung
Choi, Jae Woong
Lee, Kyung-Tae
Hong, Hee-Do
author_sort Cho, Chang-Won
collection PubMed
description Polysaccharides isolated from various plants are considered precious bioactive materials owing to their potent biological activities. Previously, we prepared a polysaccharide fraction (BLE0) isolated from young barley leaves (Hordeum vulgare L.), demonstrating its anti-osteoporotic and immunostimulatory activities. However, data regarding BLE0 toxicity is lacking. To establish its safety, in vitro genotoxicity (chromosomal aberration and bacterial reverse mutation assays) and acute oral toxicity assays were conducted. In the in vitro genotoxicity assays, bacterial reverse mutation and chromosomal aberration assays showed that BLE0 possessed no mutagenicity or clastogenicity. Furthermore, the median lethal dose (LD(50)) of BLE0 was higher than 5000 mg/kg in female and male Sprague-Dawley (SD) rats and no adverse effects were observed in terms of mortality and abnormal changes in clinical signs (body weight and necropsy). Based on these results, BLE0 was found to be safe with regards to genotoxicity under our test conditions, demonstrating no acute oral toxicity up to 5000 mg/kg in SD rats.
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spelling pubmed-73534722020-07-15 Acute Oral Toxicity and Genotoxicity of Polysaccharide Fraction from Young Barley Leaves (Hordeum vulgare L.) Cho, Chang-Won Song, Young-Ran Lim, Won-Chul Hwang, Youn-Hwan Rhee, Young Kyoung Choi, Jae Woong Lee, Kyung-Tae Hong, Hee-Do Foods Communication Polysaccharides isolated from various plants are considered precious bioactive materials owing to their potent biological activities. Previously, we prepared a polysaccharide fraction (BLE0) isolated from young barley leaves (Hordeum vulgare L.), demonstrating its anti-osteoporotic and immunostimulatory activities. However, data regarding BLE0 toxicity is lacking. To establish its safety, in vitro genotoxicity (chromosomal aberration and bacterial reverse mutation assays) and acute oral toxicity assays were conducted. In the in vitro genotoxicity assays, bacterial reverse mutation and chromosomal aberration assays showed that BLE0 possessed no mutagenicity or clastogenicity. Furthermore, the median lethal dose (LD(50)) of BLE0 was higher than 5000 mg/kg in female and male Sprague-Dawley (SD) rats and no adverse effects were observed in terms of mortality and abnormal changes in clinical signs (body weight and necropsy). Based on these results, BLE0 was found to be safe with regards to genotoxicity under our test conditions, demonstrating no acute oral toxicity up to 5000 mg/kg in SD rats. MDPI 2020-06-19 /pmc/articles/PMC7353472/ /pubmed/32575580 http://dx.doi.org/10.3390/foods9060809 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Communication
Cho, Chang-Won
Song, Young-Ran
Lim, Won-Chul
Hwang, Youn-Hwan
Rhee, Young Kyoung
Choi, Jae Woong
Lee, Kyung-Tae
Hong, Hee-Do
Acute Oral Toxicity and Genotoxicity of Polysaccharide Fraction from Young Barley Leaves (Hordeum vulgare L.)
title Acute Oral Toxicity and Genotoxicity of Polysaccharide Fraction from Young Barley Leaves (Hordeum vulgare L.)
title_full Acute Oral Toxicity and Genotoxicity of Polysaccharide Fraction from Young Barley Leaves (Hordeum vulgare L.)
title_fullStr Acute Oral Toxicity and Genotoxicity of Polysaccharide Fraction from Young Barley Leaves (Hordeum vulgare L.)
title_full_unstemmed Acute Oral Toxicity and Genotoxicity of Polysaccharide Fraction from Young Barley Leaves (Hordeum vulgare L.)
title_short Acute Oral Toxicity and Genotoxicity of Polysaccharide Fraction from Young Barley Leaves (Hordeum vulgare L.)
title_sort acute oral toxicity and genotoxicity of polysaccharide fraction from young barley leaves (hordeum vulgare l.)
topic Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7353472/
https://www.ncbi.nlm.nih.gov/pubmed/32575580
http://dx.doi.org/10.3390/foods9060809
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