Cargando…

Coating Medpor(®) Implant with Tissue-Engineered Elastic Cartilage

Inert biomaterials used for auricular reconstruction, which is one of the most challenging and diverse tasks in craniofacial or head and neck surgery, often cause problems such as capsule formation, infection, and skin extrusion. To solve these problems, scaffold consisting of inert biomaterial, hig...

Descripción completa

Detalles Bibliográficos
Autores principales: Lee, Dong Joon, Kwon, Jane, Kim, Yong-Il, Kwon, Yong Hoon, Min, Samuel, Shin, Hae Won
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7353498/
https://www.ncbi.nlm.nih.gov/pubmed/32455861
http://dx.doi.org/10.3390/jfb11020034
_version_ 1783557890016018432
author Lee, Dong Joon
Kwon, Jane
Kim, Yong-Il
Kwon, Yong Hoon
Min, Samuel
Shin, Hae Won
author_facet Lee, Dong Joon
Kwon, Jane
Kim, Yong-Il
Kwon, Yong Hoon
Min, Samuel
Shin, Hae Won
author_sort Lee, Dong Joon
collection PubMed
description Inert biomaterials used for auricular reconstruction, which is one of the most challenging and diverse tasks in craniofacial or head and neck surgery, often cause problems such as capsule formation, infection, and skin extrusion. To solve these problems, scaffold consisting of inert biomaterial, high-density polyethylene (Medpor(®)) encapsulated with neocartilage, biodegradable poly(DL-lactic-co-glycolic acid) (PLGA) was created using a tissue engineering strategy. PLGA scaffold without Medpor(®) was created to serve as the control. Scaffolds were vacuum-seeded with rabbit chondrocytes, freshly isolated from the ear by enzymatic digestion. Then, cell-seeded scaffolds were implanted subcutaneously in the dorsal pockets of nude mice. After 12 weeks, explants were analyzed by histological, biochemical, and mechanical evaluations. Although the PLGA group resulted in neocartilage formation, the PLGA–Medpor(®) group demonstrated improved outcome with the formation of well-surrounded cartilage around the implants with higher mechanical strength than the PLGA group, indicating that Medpor(®) has an influence on the structural strength of engineered cartilage. The presence of collagen and elastin fibers was evident in the histological section in both groups. These results demonstrated a novel method of coating implant material with engineered cartilage to overcome the limitations of using biodegradable scaffold in cartilage tissue regeneration. By utilizing the patient’s own chondrocytes, our proposed method may broaden the choice of implant materials while minimizing side effects and immune reaction for the future medical application.
format Online
Article
Text
id pubmed-7353498
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-73534982020-07-15 Coating Medpor(®) Implant with Tissue-Engineered Elastic Cartilage Lee, Dong Joon Kwon, Jane Kim, Yong-Il Kwon, Yong Hoon Min, Samuel Shin, Hae Won J Funct Biomater Article Inert biomaterials used for auricular reconstruction, which is one of the most challenging and diverse tasks in craniofacial or head and neck surgery, often cause problems such as capsule formation, infection, and skin extrusion. To solve these problems, scaffold consisting of inert biomaterial, high-density polyethylene (Medpor(®)) encapsulated with neocartilage, biodegradable poly(DL-lactic-co-glycolic acid) (PLGA) was created using a tissue engineering strategy. PLGA scaffold without Medpor(®) was created to serve as the control. Scaffolds were vacuum-seeded with rabbit chondrocytes, freshly isolated from the ear by enzymatic digestion. Then, cell-seeded scaffolds were implanted subcutaneously in the dorsal pockets of nude mice. After 12 weeks, explants were analyzed by histological, biochemical, and mechanical evaluations. Although the PLGA group resulted in neocartilage formation, the PLGA–Medpor(®) group demonstrated improved outcome with the formation of well-surrounded cartilage around the implants with higher mechanical strength than the PLGA group, indicating that Medpor(®) has an influence on the structural strength of engineered cartilage. The presence of collagen and elastin fibers was evident in the histological section in both groups. These results demonstrated a novel method of coating implant material with engineered cartilage to overcome the limitations of using biodegradable scaffold in cartilage tissue regeneration. By utilizing the patient’s own chondrocytes, our proposed method may broaden the choice of implant materials while minimizing side effects and immune reaction for the future medical application. MDPI 2020-05-22 /pmc/articles/PMC7353498/ /pubmed/32455861 http://dx.doi.org/10.3390/jfb11020034 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Lee, Dong Joon
Kwon, Jane
Kim, Yong-Il
Kwon, Yong Hoon
Min, Samuel
Shin, Hae Won
Coating Medpor(®) Implant with Tissue-Engineered Elastic Cartilage
title Coating Medpor(®) Implant with Tissue-Engineered Elastic Cartilage
title_full Coating Medpor(®) Implant with Tissue-Engineered Elastic Cartilage
title_fullStr Coating Medpor(®) Implant with Tissue-Engineered Elastic Cartilage
title_full_unstemmed Coating Medpor(®) Implant with Tissue-Engineered Elastic Cartilage
title_short Coating Medpor(®) Implant with Tissue-Engineered Elastic Cartilage
title_sort coating medpor(®) implant with tissue-engineered elastic cartilage
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7353498/
https://www.ncbi.nlm.nih.gov/pubmed/32455861
http://dx.doi.org/10.3390/jfb11020034
work_keys_str_mv AT leedongjoon coatingmedporimplantwithtissueengineeredelasticcartilage
AT kwonjane coatingmedporimplantwithtissueengineeredelasticcartilage
AT kimyongil coatingmedporimplantwithtissueengineeredelasticcartilage
AT kwonyonghoon coatingmedporimplantwithtissueengineeredelasticcartilage
AT minsamuel coatingmedporimplantwithtissueengineeredelasticcartilage
AT shinhaewon coatingmedporimplantwithtissueengineeredelasticcartilage