Cargando…

Internalization of Metal–Organic Framework Nanoparticles in Human Vascular Cells: Implications for Cardiovascular Disease Therapy

Cardiovascular diseases (CVDs) are the leading cause of morbidity and mortality worldwide. Alteration of endothelial cells and the underlying vasculature plays a central role in the pathogenesis of various CVDs. The application of nanoscale materials such as nanoparticles in biomedicine has opened n...

Descripción completa

Detalles Bibliográficos
Autores principales: Al-Ansari, Dana E., Mohamed, Nura A., Marei, Isra, Zekri, Atef, Kameno, Yu, Davies, Robert P., Lickiss, Paul D., Rahman, Md Mizanur, Abou-Saleh, Haissam
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7353612/
https://www.ncbi.nlm.nih.gov/pubmed/32471187
http://dx.doi.org/10.3390/nano10061028
_version_ 1783557917178331136
author Al-Ansari, Dana E.
Mohamed, Nura A.
Marei, Isra
Zekri, Atef
Kameno, Yu
Davies, Robert P.
Lickiss, Paul D.
Rahman, Md Mizanur
Abou-Saleh, Haissam
author_facet Al-Ansari, Dana E.
Mohamed, Nura A.
Marei, Isra
Zekri, Atef
Kameno, Yu
Davies, Robert P.
Lickiss, Paul D.
Rahman, Md Mizanur
Abou-Saleh, Haissam
author_sort Al-Ansari, Dana E.
collection PubMed
description Cardiovascular diseases (CVDs) are the leading cause of morbidity and mortality worldwide. Alteration of endothelial cells and the underlying vasculature plays a central role in the pathogenesis of various CVDs. The application of nanoscale materials such as nanoparticles in biomedicine has opened new horizons in the treatment of CVDs. We have previously shown that the iron metal–organic framework nanoparticle, Materials Institut Lavoisier-89 (nanoMIL-89) represents a viable vehicle for future drug delivery of pulmonary arterial hypertension. In this study, we have assessed the cellular uptake of nanoMIL-89 in pulmonary artery endothelial and smooth muscle cells using microscopy imaging techniques. We also tested the cellular responses to nanoMIL-89 using molecular and cellular assays. Microscopic images showed cellular internalization of nanoMIL-89, packaging into endocytic vesicles, and passing to daughter cells during mitosis. Moreover, nanoMIL-89 showed anti-inflammatory activity without any significant cytotoxicity. Our results indicate that nanoMIL-89 formulation may offer promising therapeutic opportunities and set forth a new prototype for drug delivery not only in CVDs, but also for other diseases yet incurable, such as diabetes and cancer.
format Online
Article
Text
id pubmed-7353612
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-73536122020-07-21 Internalization of Metal–Organic Framework Nanoparticles in Human Vascular Cells: Implications for Cardiovascular Disease Therapy Al-Ansari, Dana E. Mohamed, Nura A. Marei, Isra Zekri, Atef Kameno, Yu Davies, Robert P. Lickiss, Paul D. Rahman, Md Mizanur Abou-Saleh, Haissam Nanomaterials (Basel) Article Cardiovascular diseases (CVDs) are the leading cause of morbidity and mortality worldwide. Alteration of endothelial cells and the underlying vasculature plays a central role in the pathogenesis of various CVDs. The application of nanoscale materials such as nanoparticles in biomedicine has opened new horizons in the treatment of CVDs. We have previously shown that the iron metal–organic framework nanoparticle, Materials Institut Lavoisier-89 (nanoMIL-89) represents a viable vehicle for future drug delivery of pulmonary arterial hypertension. In this study, we have assessed the cellular uptake of nanoMIL-89 in pulmonary artery endothelial and smooth muscle cells using microscopy imaging techniques. We also tested the cellular responses to nanoMIL-89 using molecular and cellular assays. Microscopic images showed cellular internalization of nanoMIL-89, packaging into endocytic vesicles, and passing to daughter cells during mitosis. Moreover, nanoMIL-89 showed anti-inflammatory activity without any significant cytotoxicity. Our results indicate that nanoMIL-89 formulation may offer promising therapeutic opportunities and set forth a new prototype for drug delivery not only in CVDs, but also for other diseases yet incurable, such as diabetes and cancer. MDPI 2020-05-27 /pmc/articles/PMC7353612/ /pubmed/32471187 http://dx.doi.org/10.3390/nano10061028 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Al-Ansari, Dana E.
Mohamed, Nura A.
Marei, Isra
Zekri, Atef
Kameno, Yu
Davies, Robert P.
Lickiss, Paul D.
Rahman, Md Mizanur
Abou-Saleh, Haissam
Internalization of Metal–Organic Framework Nanoparticles in Human Vascular Cells: Implications for Cardiovascular Disease Therapy
title Internalization of Metal–Organic Framework Nanoparticles in Human Vascular Cells: Implications for Cardiovascular Disease Therapy
title_full Internalization of Metal–Organic Framework Nanoparticles in Human Vascular Cells: Implications for Cardiovascular Disease Therapy
title_fullStr Internalization of Metal–Organic Framework Nanoparticles in Human Vascular Cells: Implications for Cardiovascular Disease Therapy
title_full_unstemmed Internalization of Metal–Organic Framework Nanoparticles in Human Vascular Cells: Implications for Cardiovascular Disease Therapy
title_short Internalization of Metal–Organic Framework Nanoparticles in Human Vascular Cells: Implications for Cardiovascular Disease Therapy
title_sort internalization of metal–organic framework nanoparticles in human vascular cells: implications for cardiovascular disease therapy
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7353612/
https://www.ncbi.nlm.nih.gov/pubmed/32471187
http://dx.doi.org/10.3390/nano10061028
work_keys_str_mv AT alansaridanae internalizationofmetalorganicframeworknanoparticlesinhumanvascularcellsimplicationsforcardiovasculardiseasetherapy
AT mohamednuraa internalizationofmetalorganicframeworknanoparticlesinhumanvascularcellsimplicationsforcardiovasculardiseasetherapy
AT mareiisra internalizationofmetalorganicframeworknanoparticlesinhumanvascularcellsimplicationsforcardiovasculardiseasetherapy
AT zekriatef internalizationofmetalorganicframeworknanoparticlesinhumanvascularcellsimplicationsforcardiovasculardiseasetherapy
AT kamenoyu internalizationofmetalorganicframeworknanoparticlesinhumanvascularcellsimplicationsforcardiovasculardiseasetherapy
AT daviesrobertp internalizationofmetalorganicframeworknanoparticlesinhumanvascularcellsimplicationsforcardiovasculardiseasetherapy
AT lickisspauld internalizationofmetalorganicframeworknanoparticlesinhumanvascularcellsimplicationsforcardiovasculardiseasetherapy
AT rahmanmdmizanur internalizationofmetalorganicframeworknanoparticlesinhumanvascularcellsimplicationsforcardiovasculardiseasetherapy
AT abousalehhaissam internalizationofmetalorganicframeworknanoparticlesinhumanvascularcellsimplicationsforcardiovasculardiseasetherapy