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Tandem mass tag-based (TMT) quantitative proteomics analysis reveals the response of fine roots to drought stress in cotton (Gossypium hirsutum L.)

BACKGROUND: Cotton (Gossypium hirsutum L.) is one of the most important cash crops worldwide. Fine roots are the central part of the root system that contributes to plant water and nutrient uptake. However, the mechanisms underlying the response of cotton fine roots to soil drought remains unclear....

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Autores principales: Xiao, Shuang, Liu, Liantao, Zhang, Yongjiang, Sun, Hongchun, Zhang, Ke, Bai, Zhiying, Dong, Hezhong, Liu, Yuchun, Li, Cundong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7353779/
https://www.ncbi.nlm.nih.gov/pubmed/32652934
http://dx.doi.org/10.1186/s12870-020-02531-z
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author Xiao, Shuang
Liu, Liantao
Zhang, Yongjiang
Sun, Hongchun
Zhang, Ke
Bai, Zhiying
Dong, Hezhong
Liu, Yuchun
Li, Cundong
author_facet Xiao, Shuang
Liu, Liantao
Zhang, Yongjiang
Sun, Hongchun
Zhang, Ke
Bai, Zhiying
Dong, Hezhong
Liu, Yuchun
Li, Cundong
author_sort Xiao, Shuang
collection PubMed
description BACKGROUND: Cotton (Gossypium hirsutum L.) is one of the most important cash crops worldwide. Fine roots are the central part of the root system that contributes to plant water and nutrient uptake. However, the mechanisms underlying the response of cotton fine roots to soil drought remains unclear. To elucidate the proteomic changes in fine roots of cotton plants under drought stress, 70–75% and 40–45% soil relative water content treatments were imposed on control (CK) and drought stress (DS) groups, respectively. Then, tandem mass tags (TMT) technology was used to determine the proteome profiles of fine root tissue samples. RESULTS: Drought significantly decreased the value of average root diameter of cotton seedlings, whereas the total root length and the activities of antioxidases were increased. To study the molecular mechanisms underlying drought response further, the proteome differences between tissues under CK and DS treatments were compared pairwise at 0, 30, and 45 DAD (days after drought stress). In total, 118 differentially expressed proteins (DEPs) were up-regulated and 105 were down-regulated in the ‘DS30 versus CK30’ comparison; 662 DEPs were up-regulated, and 611 were down-regulated in the ‘DS45 versus CK45’ comparison. The functions of these DEPs were classified according to their pathways. Under early stage drought (30 DAD), some DEPs involved in the ‘Cutin, suberin, and wax synthesis’ pathway were up-regulated, while the down-regulated DEPs were mainly enriched within the ‘Monoterpenoid biosynthesis’ pathway. Forty-five days of soil drought had a greater impact on DEPs involved in metabolism. Many proteins involving ‘Carbohydrate metabolism,’ ‘Energy metabolism,’ ‘Fatty acid metabolism,’ ‘Amino acid metabolism,’ and ‘Secondary metabolite biosynthesis’ were identified as DEPs. Additionally, proteins related to ion transport, stress/defense, and phytohormones were also shown to play roles in determining the fine root growth of cotton plants under drought stress. CONCLUSIONS: Our study identified potential biological pathways and drought-responsive proteins related to stress/defense responses and plant hormone metabolism under drought stress. Collectively, our results provide new insights for further improving drought tolerance in cotton and other crops.
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spelling pubmed-73537792020-07-15 Tandem mass tag-based (TMT) quantitative proteomics analysis reveals the response of fine roots to drought stress in cotton (Gossypium hirsutum L.) Xiao, Shuang Liu, Liantao Zhang, Yongjiang Sun, Hongchun Zhang, Ke Bai, Zhiying Dong, Hezhong Liu, Yuchun Li, Cundong BMC Plant Biol Research Article BACKGROUND: Cotton (Gossypium hirsutum L.) is one of the most important cash crops worldwide. Fine roots are the central part of the root system that contributes to plant water and nutrient uptake. However, the mechanisms underlying the response of cotton fine roots to soil drought remains unclear. To elucidate the proteomic changes in fine roots of cotton plants under drought stress, 70–75% and 40–45% soil relative water content treatments were imposed on control (CK) and drought stress (DS) groups, respectively. Then, tandem mass tags (TMT) technology was used to determine the proteome profiles of fine root tissue samples. RESULTS: Drought significantly decreased the value of average root diameter of cotton seedlings, whereas the total root length and the activities of antioxidases were increased. To study the molecular mechanisms underlying drought response further, the proteome differences between tissues under CK and DS treatments were compared pairwise at 0, 30, and 45 DAD (days after drought stress). In total, 118 differentially expressed proteins (DEPs) were up-regulated and 105 were down-regulated in the ‘DS30 versus CK30’ comparison; 662 DEPs were up-regulated, and 611 were down-regulated in the ‘DS45 versus CK45’ comparison. The functions of these DEPs were classified according to their pathways. Under early stage drought (30 DAD), some DEPs involved in the ‘Cutin, suberin, and wax synthesis’ pathway were up-regulated, while the down-regulated DEPs were mainly enriched within the ‘Monoterpenoid biosynthesis’ pathway. Forty-five days of soil drought had a greater impact on DEPs involved in metabolism. Many proteins involving ‘Carbohydrate metabolism,’ ‘Energy metabolism,’ ‘Fatty acid metabolism,’ ‘Amino acid metabolism,’ and ‘Secondary metabolite biosynthesis’ were identified as DEPs. Additionally, proteins related to ion transport, stress/defense, and phytohormones were also shown to play roles in determining the fine root growth of cotton plants under drought stress. CONCLUSIONS: Our study identified potential biological pathways and drought-responsive proteins related to stress/defense responses and plant hormone metabolism under drought stress. Collectively, our results provide new insights for further improving drought tolerance in cotton and other crops. BioMed Central 2020-07-11 /pmc/articles/PMC7353779/ /pubmed/32652934 http://dx.doi.org/10.1186/s12870-020-02531-z Text en © The Author(s) 2020 Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research Article
Xiao, Shuang
Liu, Liantao
Zhang, Yongjiang
Sun, Hongchun
Zhang, Ke
Bai, Zhiying
Dong, Hezhong
Liu, Yuchun
Li, Cundong
Tandem mass tag-based (TMT) quantitative proteomics analysis reveals the response of fine roots to drought stress in cotton (Gossypium hirsutum L.)
title Tandem mass tag-based (TMT) quantitative proteomics analysis reveals the response of fine roots to drought stress in cotton (Gossypium hirsutum L.)
title_full Tandem mass tag-based (TMT) quantitative proteomics analysis reveals the response of fine roots to drought stress in cotton (Gossypium hirsutum L.)
title_fullStr Tandem mass tag-based (TMT) quantitative proteomics analysis reveals the response of fine roots to drought stress in cotton (Gossypium hirsutum L.)
title_full_unstemmed Tandem mass tag-based (TMT) quantitative proteomics analysis reveals the response of fine roots to drought stress in cotton (Gossypium hirsutum L.)
title_short Tandem mass tag-based (TMT) quantitative proteomics analysis reveals the response of fine roots to drought stress in cotton (Gossypium hirsutum L.)
title_sort tandem mass tag-based (tmt) quantitative proteomics analysis reveals the response of fine roots to drought stress in cotton (gossypium hirsutum l.)
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7353779/
https://www.ncbi.nlm.nih.gov/pubmed/32652934
http://dx.doi.org/10.1186/s12870-020-02531-z
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