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Allogenic Bone Graft Enriched by Periosteal Stem Cell and Growth Factors for Osteogenesis in Critical Size Bone Defect in Rabbit Model: Histopathological and Radiological Evaluation

BACKGROUND & OBJECTIVE: This study aimed to investigate the effect of decellularized allogeneic bone graft enriched by periosteal stem cells (PSCs) and growth factors on the bone repair process in a rabbit model, which could be used in many orthopedic procedures. METHODS: In this experimental st...

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Autores principales: Hassibi, Hadi, Farsinejad, Alireza, Dabiri, Shahriar, Voosough, Darioush, Mortezaeizadeh, Abbas, Kheirandish, Reza, Azari, Omid
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Iranian Society of Pathology 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7354065/
https://www.ncbi.nlm.nih.gov/pubmed/32754216
http://dx.doi.org/10.30699/ijp.2020.101715.2013
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author Hassibi, Hadi
Farsinejad, Alireza
Dabiri, Shahriar
Voosough, Darioush
Mortezaeizadeh, Abbas
Kheirandish, Reza
Azari, Omid
author_facet Hassibi, Hadi
Farsinejad, Alireza
Dabiri, Shahriar
Voosough, Darioush
Mortezaeizadeh, Abbas
Kheirandish, Reza
Azari, Omid
author_sort Hassibi, Hadi
collection PubMed
description BACKGROUND & OBJECTIVE: This study aimed to investigate the effect of decellularized allogeneic bone graft enriched by periosteal stem cells (PSCs) and growth factors on the bone repair process in a rabbit model, which could be used in many orthopedic procedures. METHODS: In this experimental study, a critical size defect (CSD) (10 mm) was created in the radial diaphysis of 40 rabbits. In group A, the defect was left intact with no medical intervention. In group B, the defect was filled by a decellularized bone graft. In group C, the defect was implanted by a decellularized bone graft enriched with platelet growth factors. In group D, the defect was treated by a decellularized bone graft seeded by periosteal mesenchymal stem cells (MSCs). Also, in group E, the defect was filled by a decellularized bone graft enriched with platelet growth factors and periosteal MSCs. Radiological evaluation was done on the first day and then in the second, fourth, and eighth weeks after the operation. The specimens were harvested on the 28th and 56th postoperative days and evaluated for histopathological criteria. RESULTS: The radiologic and microscopic analysis of the healing process in bone defects of the treated groups (C, D, and E) revealed more advanced repair criteria than those of groups A and B significantly (P<0.05). CONCLUSION: Based on this study, it appears that implantation of concentrated PSCs in combination with growth factors and allogeneic cortical bone graft is an effective therapy for the repair of large bone defects.
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spelling pubmed-73540652020-08-03 Allogenic Bone Graft Enriched by Periosteal Stem Cell and Growth Factors for Osteogenesis in Critical Size Bone Defect in Rabbit Model: Histopathological and Radiological Evaluation Hassibi, Hadi Farsinejad, Alireza Dabiri, Shahriar Voosough, Darioush Mortezaeizadeh, Abbas Kheirandish, Reza Azari, Omid Iran J Pathol Original Article BACKGROUND & OBJECTIVE: This study aimed to investigate the effect of decellularized allogeneic bone graft enriched by periosteal stem cells (PSCs) and growth factors on the bone repair process in a rabbit model, which could be used in many orthopedic procedures. METHODS: In this experimental study, a critical size defect (CSD) (10 mm) was created in the radial diaphysis of 40 rabbits. In group A, the defect was left intact with no medical intervention. In group B, the defect was filled by a decellularized bone graft. In group C, the defect was implanted by a decellularized bone graft enriched with platelet growth factors. In group D, the defect was treated by a decellularized bone graft seeded by periosteal mesenchymal stem cells (MSCs). Also, in group E, the defect was filled by a decellularized bone graft enriched with platelet growth factors and periosteal MSCs. Radiological evaluation was done on the first day and then in the second, fourth, and eighth weeks after the operation. The specimens were harvested on the 28th and 56th postoperative days and evaluated for histopathological criteria. RESULTS: The radiologic and microscopic analysis of the healing process in bone defects of the treated groups (C, D, and E) revealed more advanced repair criteria than those of groups A and B significantly (P<0.05). CONCLUSION: Based on this study, it appears that implantation of concentrated PSCs in combination with growth factors and allogeneic cortical bone graft is an effective therapy for the repair of large bone defects. Iranian Society of Pathology 2020 2020-04-21 /pmc/articles/PMC7354065/ /pubmed/32754216 http://dx.doi.org/10.30699/ijp.2020.101715.2013 Text en This is an open-access article distributed under the terms of the Creative Commons Attribution-noncommercial 4.0 International License, (https://creativecommons.org/licenses/by-nc/4.0/) which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited.
spellingShingle Original Article
Hassibi, Hadi
Farsinejad, Alireza
Dabiri, Shahriar
Voosough, Darioush
Mortezaeizadeh, Abbas
Kheirandish, Reza
Azari, Omid
Allogenic Bone Graft Enriched by Periosteal Stem Cell and Growth Factors for Osteogenesis in Critical Size Bone Defect in Rabbit Model: Histopathological and Radiological Evaluation
title Allogenic Bone Graft Enriched by Periosteal Stem Cell and Growth Factors for Osteogenesis in Critical Size Bone Defect in Rabbit Model: Histopathological and Radiological Evaluation
title_full Allogenic Bone Graft Enriched by Periosteal Stem Cell and Growth Factors for Osteogenesis in Critical Size Bone Defect in Rabbit Model: Histopathological and Radiological Evaluation
title_fullStr Allogenic Bone Graft Enriched by Periosteal Stem Cell and Growth Factors for Osteogenesis in Critical Size Bone Defect in Rabbit Model: Histopathological and Radiological Evaluation
title_full_unstemmed Allogenic Bone Graft Enriched by Periosteal Stem Cell and Growth Factors for Osteogenesis in Critical Size Bone Defect in Rabbit Model: Histopathological and Radiological Evaluation
title_short Allogenic Bone Graft Enriched by Periosteal Stem Cell and Growth Factors for Osteogenesis in Critical Size Bone Defect in Rabbit Model: Histopathological and Radiological Evaluation
title_sort allogenic bone graft enriched by periosteal stem cell and growth factors for osteogenesis in critical size bone defect in rabbit model: histopathological and radiological evaluation
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7354065/
https://www.ncbi.nlm.nih.gov/pubmed/32754216
http://dx.doi.org/10.30699/ijp.2020.101715.2013
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