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原发免疫性血小板减少症患者NK细胞相关免疫负调控的研究

OBJECTIVE: To investigate the levels of NK cells and their relevant cytokines (IL-10, TGF-β and IFN-γ) in patients with primary immune thrombocytopenia (ITP). METHODS: All samples were obtained from 42 patients (22 newly diagnosed and 20 in remission) and 20 healthy volunteers. The levels of IL-10 a...

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Formato: Online Artículo Texto
Lenguaje:English
Publicado: Editorial office of Chinese Journal of Hematology 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7354183/
https://www.ncbi.nlm.nih.gov/pubmed/28565739
http://dx.doi.org/10.3760/cma.j.issn.0253-2727.2017.05.009
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description OBJECTIVE: To investigate the levels of NK cells and their relevant cytokines (IL-10, TGF-β and IFN-γ) in patients with primary immune thrombocytopenia (ITP). METHODS: All samples were obtained from 42 patients (22 newly diagnosed and 20 in remission) and 20 healthy volunteers. The levels of IL-10 and IFN-γ in blood serum were detected by enzyme-linked immunosorbent assay (ELISA). The percentage of CD3(−) CD56(+) NK cell, CD3(−) CD56(bright) CD16(−) NK cell, CD3(−) CD56(dim) CD16(+) NK cell in peripheral blood lymphocyte were detected by flow cytometry. The NK cells were isolated by immunomagnetic microbeads. The mRNA expression levels of IL-10, TGF-β, and IFN-γ in NK cells were detected by real-time fluorescent quantitative PCR. Correlation between the above measured results was analyzed. RESULTS: ①The blood serum level of IFN-γ in newly diagnosed ITP patients [(653.0±221.6) ng/L] was higher than that in remission ITP patients [(484.4±219.5) ng/L] and healthy control [(390.9±253.5) ng/L] (P=0.022, P=0.001). The blood serum level of IL-10 in newly diagnosed ITP patients was lower than that in healthy control [(52.09±26.66) ng/L vs (79.44±38.43) ng/L, P=0.007]. ②The percentage of NK cell in newly diagnosed and remission ITP patients [(9.53±3.93) %, (9.03±3.78) %] were significantly lower than that in healthy control [(13.72±7.42) %] (P=0.013, P=0.007). The ratio of CD3(−) CD56(bright) CD16(−) NK cell/total NK cells in newly diagnosed ITP patients was higher than that in healthy control [(6.85±4.43) % vs (4.05±2.81) %, P=0.032]. The ratio of CD3(−)CD56(dim) CD16(−) NK cell/total NK cells in newly diagnosed ITP patients was lower than that in healthy control [(93.14±4.43) % vs (95.94±2.81) %, P=0.032]. ③There was no significant difference in the mRNA expression level of IFN-γ in NK cells of ITP patients and healthy control (all P>0.05). The mRNA expression levels of IL-10 and TGF-β in NK cells in newly diagnosed ITP patients were significantly higher than that in healthy control (1.82±1.32 vs 1.02±1.03, P=0.023; 2.80±2.31 vs 1.46±1.37, P=0.028). The ratio of CD3(−)CD56(bright) CD16(−) NK cell/total NK cells was positively correlated with the mRNA expression levels of IL-10, TGF-β in NK cells (r=0.424, P=0.001; r=0.432, P<0.001). CONCLUSION: NK cells may compensate for the deficiency of the number by enhancing the secretion of negative regulation cytokines, acting as “protective” roles in the disease.
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spelling pubmed-73541832020-07-16 原发免疫性血小板减少症患者NK细胞相关免疫负调控的研究 Zhonghua Xue Ye Xue Za Zhi 论著 OBJECTIVE: To investigate the levels of NK cells and their relevant cytokines (IL-10, TGF-β and IFN-γ) in patients with primary immune thrombocytopenia (ITP). METHODS: All samples were obtained from 42 patients (22 newly diagnosed and 20 in remission) and 20 healthy volunteers. The levels of IL-10 and IFN-γ in blood serum were detected by enzyme-linked immunosorbent assay (ELISA). The percentage of CD3(−) CD56(+) NK cell, CD3(−) CD56(bright) CD16(−) NK cell, CD3(−) CD56(dim) CD16(+) NK cell in peripheral blood lymphocyte were detected by flow cytometry. The NK cells were isolated by immunomagnetic microbeads. The mRNA expression levels of IL-10, TGF-β, and IFN-γ in NK cells were detected by real-time fluorescent quantitative PCR. Correlation between the above measured results was analyzed. RESULTS: ①The blood serum level of IFN-γ in newly diagnosed ITP patients [(653.0±221.6) ng/L] was higher than that in remission ITP patients [(484.4±219.5) ng/L] and healthy control [(390.9±253.5) ng/L] (P=0.022, P=0.001). The blood serum level of IL-10 in newly diagnosed ITP patients was lower than that in healthy control [(52.09±26.66) ng/L vs (79.44±38.43) ng/L, P=0.007]. ②The percentage of NK cell in newly diagnosed and remission ITP patients [(9.53±3.93) %, (9.03±3.78) %] were significantly lower than that in healthy control [(13.72±7.42) %] (P=0.013, P=0.007). The ratio of CD3(−) CD56(bright) CD16(−) NK cell/total NK cells in newly diagnosed ITP patients was higher than that in healthy control [(6.85±4.43) % vs (4.05±2.81) %, P=0.032]. The ratio of CD3(−)CD56(dim) CD16(−) NK cell/total NK cells in newly diagnosed ITP patients was lower than that in healthy control [(93.14±4.43) % vs (95.94±2.81) %, P=0.032]. ③There was no significant difference in the mRNA expression level of IFN-γ in NK cells of ITP patients and healthy control (all P>0.05). The mRNA expression levels of IL-10 and TGF-β in NK cells in newly diagnosed ITP patients were significantly higher than that in healthy control (1.82±1.32 vs 1.02±1.03, P=0.023; 2.80±2.31 vs 1.46±1.37, P=0.028). The ratio of CD3(−)CD56(bright) CD16(−) NK cell/total NK cells was positively correlated with the mRNA expression levels of IL-10, TGF-β in NK cells (r=0.424, P=0.001; r=0.432, P<0.001). CONCLUSION: NK cells may compensate for the deficiency of the number by enhancing the secretion of negative regulation cytokines, acting as “protective” roles in the disease. Editorial office of Chinese Journal of Hematology 2017-05 /pmc/articles/PMC7354183/ /pubmed/28565739 http://dx.doi.org/10.3760/cma.j.issn.0253-2727.2017.05.009 Text en 2017年版权归中华医学会所有 http://creativecommons.org/licenses/by-nc-sa/3.0/ This work is licensed under a Creative Commons Attribution 3.0 License (CC-BY-NC). The Copyright own by Publisher. Without authorization, shall not reprint, except this publication article, shall not use this publication format design. Unless otherwise stated, all articles published in this journal do not represent the views of the Chinese Medical Association or the editorial board of this journal.
spellingShingle 论著
原发免疫性血小板减少症患者NK细胞相关免疫负调控的研究
title 原发免疫性血小板减少症患者NK细胞相关免疫负调控的研究
title_full 原发免疫性血小板减少症患者NK细胞相关免疫负调控的研究
title_fullStr 原发免疫性血小板减少症患者NK细胞相关免疫负调控的研究
title_full_unstemmed 原发免疫性血小板减少症患者NK细胞相关免疫负调控的研究
title_short 原发免疫性血小板减少症患者NK细胞相关免疫负调控的研究
title_sort 原发免疫性血小板减少症患者nk细胞相关免疫负调控的研究
topic 论著
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7354183/
https://www.ncbi.nlm.nih.gov/pubmed/28565739
http://dx.doi.org/10.3760/cma.j.issn.0253-2727.2017.05.009
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