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WSL9 Encodes an HNH Endonuclease Domain-Containing Protein that Is Essential for Early Chloroplast Development in Rice

BACKGROUND: The plant chloroplast is essential for photosynthesis and other cellular processes, but an understanding of the biological mechanisms of plant chloroplast development are incomplete. RESULTS: A new temperature-sensitive white stripe leaf 9(wsl9) rice mutant is described. The mutant devel...

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Autores principales: Zhu, Xingjie, Mou, Changling, Zhang, Fulin, Huang, Yunshuai, Yang, Chunyan, Ji, Jingli, Liu, Xi, Cao, Penghui, Nguyen, Thanhliem, Lan, Jie, Zhou, Chunlei, Liu, Shijia, Jiang, Ling, Wan, Jianmin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer US 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7354284/
https://www.ncbi.nlm.nih.gov/pubmed/32654074
http://dx.doi.org/10.1186/s12284-020-00407-2
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author Zhu, Xingjie
Mou, Changling
Zhang, Fulin
Huang, Yunshuai
Yang, Chunyan
Ji, Jingli
Liu, Xi
Cao, Penghui
Nguyen, Thanhliem
Lan, Jie
Zhou, Chunlei
Liu, Shijia
Jiang, Ling
Wan, Jianmin
author_facet Zhu, Xingjie
Mou, Changling
Zhang, Fulin
Huang, Yunshuai
Yang, Chunyan
Ji, Jingli
Liu, Xi
Cao, Penghui
Nguyen, Thanhliem
Lan, Jie
Zhou, Chunlei
Liu, Shijia
Jiang, Ling
Wan, Jianmin
author_sort Zhu, Xingjie
collection PubMed
description BACKGROUND: The plant chloroplast is essential for photosynthesis and other cellular processes, but an understanding of the biological mechanisms of plant chloroplast development are incomplete. RESULTS: A new temperature-sensitive white stripe leaf 9(wsl9) rice mutant is described. The mutant develops white stripes during early leaf development, but becomes green after the three-leaf stage under field conditions. The wsl9 mutant was albinic when grown at low temperature. Gene mapping of the WSL9 locus, together with complementation tests indicated that WSL9 encodes a novel protein with an HNH domain. WSL9 was expressed in various tissues. Under low temperature, the wsl9 mutation caused defects in splicing of rpl2, but increased the editing efficiency of rpoB. Expression levels of plastid genome-encoded genes, which are transcribed by plastid-coded RNA polymerase (PEP), chloroplast development genes and photosynthesis-related genes were altered in the wsl9 mutant. CONCLUSION: WSL9 encodes an HNH endonuclease domain-containing protein that is essential for early chloroplast development. Our study provides opportunities for further research on regulatory mechanisms of chloroplast development in rice.
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spelling pubmed-73542842020-07-14 WSL9 Encodes an HNH Endonuclease Domain-Containing Protein that Is Essential for Early Chloroplast Development in Rice Zhu, Xingjie Mou, Changling Zhang, Fulin Huang, Yunshuai Yang, Chunyan Ji, Jingli Liu, Xi Cao, Penghui Nguyen, Thanhliem Lan, Jie Zhou, Chunlei Liu, Shijia Jiang, Ling Wan, Jianmin Rice (N Y) Original Article BACKGROUND: The plant chloroplast is essential for photosynthesis and other cellular processes, but an understanding of the biological mechanisms of plant chloroplast development are incomplete. RESULTS: A new temperature-sensitive white stripe leaf 9(wsl9) rice mutant is described. The mutant develops white stripes during early leaf development, but becomes green after the three-leaf stage under field conditions. The wsl9 mutant was albinic when grown at low temperature. Gene mapping of the WSL9 locus, together with complementation tests indicated that WSL9 encodes a novel protein with an HNH domain. WSL9 was expressed in various tissues. Under low temperature, the wsl9 mutation caused defects in splicing of rpl2, but increased the editing efficiency of rpoB. Expression levels of plastid genome-encoded genes, which are transcribed by plastid-coded RNA polymerase (PEP), chloroplast development genes and photosynthesis-related genes were altered in the wsl9 mutant. CONCLUSION: WSL9 encodes an HNH endonuclease domain-containing protein that is essential for early chloroplast development. Our study provides opportunities for further research on regulatory mechanisms of chloroplast development in rice. Springer US 2020-07-11 /pmc/articles/PMC7354284/ /pubmed/32654074 http://dx.doi.org/10.1186/s12284-020-00407-2 Text en © The Author(s) 2020 Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Original Article
Zhu, Xingjie
Mou, Changling
Zhang, Fulin
Huang, Yunshuai
Yang, Chunyan
Ji, Jingli
Liu, Xi
Cao, Penghui
Nguyen, Thanhliem
Lan, Jie
Zhou, Chunlei
Liu, Shijia
Jiang, Ling
Wan, Jianmin
WSL9 Encodes an HNH Endonuclease Domain-Containing Protein that Is Essential for Early Chloroplast Development in Rice
title WSL9 Encodes an HNH Endonuclease Domain-Containing Protein that Is Essential for Early Chloroplast Development in Rice
title_full WSL9 Encodes an HNH Endonuclease Domain-Containing Protein that Is Essential for Early Chloroplast Development in Rice
title_fullStr WSL9 Encodes an HNH Endonuclease Domain-Containing Protein that Is Essential for Early Chloroplast Development in Rice
title_full_unstemmed WSL9 Encodes an HNH Endonuclease Domain-Containing Protein that Is Essential for Early Chloroplast Development in Rice
title_short WSL9 Encodes an HNH Endonuclease Domain-Containing Protein that Is Essential for Early Chloroplast Development in Rice
title_sort wsl9 encodes an hnh endonuclease domain-containing protein that is essential for early chloroplast development in rice
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7354284/
https://www.ncbi.nlm.nih.gov/pubmed/32654074
http://dx.doi.org/10.1186/s12284-020-00407-2
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