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Prolonged Detection of Bovine Viral Diarrhoea Virus Infection in the Semen of Bulls

Infection of bulls with bovine viral diarrhoea virus (BVDV) can result in the development of virus persistence, confined to the reproductive tract. These bulls develop a normal immune response with high neutralizing antibody titres. However, BVDV can be excreted in the semen for a prolonged period....

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Autores principales: Read, Andrew J., Gestier, Sarah, Parrish, Kate, Finlaison, Deborah S., Gu, Xingnian, O’Connor, Tiffany W., Kirkland, Peter D.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7354483/
https://www.ncbi.nlm.nih.gov/pubmed/32580423
http://dx.doi.org/10.3390/v12060674
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author Read, Andrew J.
Gestier, Sarah
Parrish, Kate
Finlaison, Deborah S.
Gu, Xingnian
O’Connor, Tiffany W.
Kirkland, Peter D.
author_facet Read, Andrew J.
Gestier, Sarah
Parrish, Kate
Finlaison, Deborah S.
Gu, Xingnian
O’Connor, Tiffany W.
Kirkland, Peter D.
author_sort Read, Andrew J.
collection PubMed
description Infection of bulls with bovine viral diarrhoea virus (BVDV) can result in the development of virus persistence, confined to the reproductive tract. These bulls develop a normal immune response with high neutralizing antibody titres. However, BVDV can be excreted in the semen for a prolonged period. Although relatively rare, in this study we describe six separate cases in bulls being prepared for admission to artificial breeding centres. Semen samples were tested in a pan-Pestivirus-reactive real-time PCR assay and viral RNA was detected in semen from five of the bulls for three to eight months after infection. In one bull, virus was detected at low levels for more than five years. This bull was found to have one small testis. When slaughtered, virus was only detected in the abnormal testis. The low levels of BVDV in the semen of these bulls were only intermittently detected by virus isolation in cell culture. This virus-contaminated semen presents a biosecurity risk and confirms the need to screen all batches of semen from bulls that have been previously infected with BVDV. The use of real-time PCR is recommended as the preferred laboratory assay for this purpose.
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spelling pubmed-73544832020-08-05 Prolonged Detection of Bovine Viral Diarrhoea Virus Infection in the Semen of Bulls Read, Andrew J. Gestier, Sarah Parrish, Kate Finlaison, Deborah S. Gu, Xingnian O’Connor, Tiffany W. Kirkland, Peter D. Viruses Article Infection of bulls with bovine viral diarrhoea virus (BVDV) can result in the development of virus persistence, confined to the reproductive tract. These bulls develop a normal immune response with high neutralizing antibody titres. However, BVDV can be excreted in the semen for a prolonged period. Although relatively rare, in this study we describe six separate cases in bulls being prepared for admission to artificial breeding centres. Semen samples were tested in a pan-Pestivirus-reactive real-time PCR assay and viral RNA was detected in semen from five of the bulls for three to eight months after infection. In one bull, virus was detected at low levels for more than five years. This bull was found to have one small testis. When slaughtered, virus was only detected in the abnormal testis. The low levels of BVDV in the semen of these bulls were only intermittently detected by virus isolation in cell culture. This virus-contaminated semen presents a biosecurity risk and confirms the need to screen all batches of semen from bulls that have been previously infected with BVDV. The use of real-time PCR is recommended as the preferred laboratory assay for this purpose. MDPI 2020-06-22 /pmc/articles/PMC7354483/ /pubmed/32580423 http://dx.doi.org/10.3390/v12060674 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Read, Andrew J.
Gestier, Sarah
Parrish, Kate
Finlaison, Deborah S.
Gu, Xingnian
O’Connor, Tiffany W.
Kirkland, Peter D.
Prolonged Detection of Bovine Viral Diarrhoea Virus Infection in the Semen of Bulls
title Prolonged Detection of Bovine Viral Diarrhoea Virus Infection in the Semen of Bulls
title_full Prolonged Detection of Bovine Viral Diarrhoea Virus Infection in the Semen of Bulls
title_fullStr Prolonged Detection of Bovine Viral Diarrhoea Virus Infection in the Semen of Bulls
title_full_unstemmed Prolonged Detection of Bovine Viral Diarrhoea Virus Infection in the Semen of Bulls
title_short Prolonged Detection of Bovine Viral Diarrhoea Virus Infection in the Semen of Bulls
title_sort prolonged detection of bovine viral diarrhoea virus infection in the semen of bulls
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7354483/
https://www.ncbi.nlm.nih.gov/pubmed/32580423
http://dx.doi.org/10.3390/v12060674
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