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Are We Ready for NGS HIV Drug Resistance Testing? The Second “Winnipeg Consensus” Symposium
HIV drug resistance is a major global challenge to successful and sustainable antiretroviral therapy. Next-generation sequencing (NGS)-based HIV drug resistance (HIVDR) assays enable more sensitive and quantitative detection of drug-resistance-associated mutations (DRMs) and outperform Sanger sequen...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7354487/ https://www.ncbi.nlm.nih.gov/pubmed/32471096 http://dx.doi.org/10.3390/v12060586 |
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author | Ji, Hezhao Sandstrom, Paul Paredes, Roger Harrigan, P. Richard Brumme, Chanson J. Avila Rios, Santiago Noguera-Julian, Marc Parkin, Neil Kantor, Rami |
author_facet | Ji, Hezhao Sandstrom, Paul Paredes, Roger Harrigan, P. Richard Brumme, Chanson J. Avila Rios, Santiago Noguera-Julian, Marc Parkin, Neil Kantor, Rami |
author_sort | Ji, Hezhao |
collection | PubMed |
description | HIV drug resistance is a major global challenge to successful and sustainable antiretroviral therapy. Next-generation sequencing (NGS)-based HIV drug resistance (HIVDR) assays enable more sensitive and quantitative detection of drug-resistance-associated mutations (DRMs) and outperform Sanger sequencing approaches in detecting lower abundance resistance mutations. While NGS is likely to become the new standard for routine HIVDR testing, many technical and knowledge gaps remain to be resolved before its generalized adoption in regular clinical care, public health, and research. Recognizing this, we conceived and launched an international symposium series on NGS HIVDR, to bring together leading experts in the field to address these issues through in-depth discussions and brainstorming. Following the first symposium in 2018 (Winnipeg, MB Canada, 21–22 February, 2018), a second “Winnipeg Consensus” symposium was held in September 2019 in Winnipeg, Canada, and was focused on external quality assurance strategies for NGS HIVDR assays. In this paper, we summarize this second symposium’s goals and highlights. |
format | Online Article Text |
id | pubmed-7354487 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-73544872020-08-05 Are We Ready for NGS HIV Drug Resistance Testing? The Second “Winnipeg Consensus” Symposium Ji, Hezhao Sandstrom, Paul Paredes, Roger Harrigan, P. Richard Brumme, Chanson J. Avila Rios, Santiago Noguera-Julian, Marc Parkin, Neil Kantor, Rami Viruses Commentary HIV drug resistance is a major global challenge to successful and sustainable antiretroviral therapy. Next-generation sequencing (NGS)-based HIV drug resistance (HIVDR) assays enable more sensitive and quantitative detection of drug-resistance-associated mutations (DRMs) and outperform Sanger sequencing approaches in detecting lower abundance resistance mutations. While NGS is likely to become the new standard for routine HIVDR testing, many technical and knowledge gaps remain to be resolved before its generalized adoption in regular clinical care, public health, and research. Recognizing this, we conceived and launched an international symposium series on NGS HIVDR, to bring together leading experts in the field to address these issues through in-depth discussions and brainstorming. Following the first symposium in 2018 (Winnipeg, MB Canada, 21–22 February, 2018), a second “Winnipeg Consensus” symposium was held in September 2019 in Winnipeg, Canada, and was focused on external quality assurance strategies for NGS HIVDR assays. In this paper, we summarize this second symposium’s goals and highlights. MDPI 2020-05-27 /pmc/articles/PMC7354487/ /pubmed/32471096 http://dx.doi.org/10.3390/v12060586 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Commentary Ji, Hezhao Sandstrom, Paul Paredes, Roger Harrigan, P. Richard Brumme, Chanson J. Avila Rios, Santiago Noguera-Julian, Marc Parkin, Neil Kantor, Rami Are We Ready for NGS HIV Drug Resistance Testing? The Second “Winnipeg Consensus” Symposium |
title | Are We Ready for NGS HIV Drug Resistance Testing? The Second “Winnipeg Consensus” Symposium |
title_full | Are We Ready for NGS HIV Drug Resistance Testing? The Second “Winnipeg Consensus” Symposium |
title_fullStr | Are We Ready for NGS HIV Drug Resistance Testing? The Second “Winnipeg Consensus” Symposium |
title_full_unstemmed | Are We Ready for NGS HIV Drug Resistance Testing? The Second “Winnipeg Consensus” Symposium |
title_short | Are We Ready for NGS HIV Drug Resistance Testing? The Second “Winnipeg Consensus” Symposium |
title_sort | are we ready for ngs hiv drug resistance testing? the second “winnipeg consensus” symposium |
topic | Commentary |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7354487/ https://www.ncbi.nlm.nih.gov/pubmed/32471096 http://dx.doi.org/10.3390/v12060586 |
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