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Novel Soil Bacterium Strain Desulfitobacterium sp. PGC-3-9 Detoxifies Trichothecene Mycotoxins in Wheat via De-Epoxidation under Aerobic and Anaerobic Conditions

Trichothecenes are the most common mycotoxins contaminating small grain cereals worldwide. The C12,13 epoxide group in the trichothecenes was identified as a toxic group posing harm to humans, farm animals, and plants. Aerobic biological de-epoxidation is considered the ideal method of controlling t...

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Autores principales: He, Wei-Jie, Shi, Meng-Meng, Yang, Peng, Huang, Tao, Yuan, Qing-Song, Yi, Shu-Yuan, Wu, Ai-Bo, Li, He-Ping, Gao, Chun-Bao, Zhang, Jing-Bo, Liao, Yu-Cai
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7354494/
https://www.ncbi.nlm.nih.gov/pubmed/32492959
http://dx.doi.org/10.3390/toxins12060363
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author He, Wei-Jie
Shi, Meng-Meng
Yang, Peng
Huang, Tao
Yuan, Qing-Song
Yi, Shu-Yuan
Wu, Ai-Bo
Li, He-Ping
Gao, Chun-Bao
Zhang, Jing-Bo
Liao, Yu-Cai
author_facet He, Wei-Jie
Shi, Meng-Meng
Yang, Peng
Huang, Tao
Yuan, Qing-Song
Yi, Shu-Yuan
Wu, Ai-Bo
Li, He-Ping
Gao, Chun-Bao
Zhang, Jing-Bo
Liao, Yu-Cai
author_sort He, Wei-Jie
collection PubMed
description Trichothecenes are the most common mycotoxins contaminating small grain cereals worldwide. The C12,13 epoxide group in the trichothecenes was identified as a toxic group posing harm to humans, farm animals, and plants. Aerobic biological de-epoxidation is considered the ideal method of controlling these types of mycotoxins. In this study, we isolated a novel trichothecene mycotoxin-de-epoxidating bacterium, Desulfitobacterium sp. PGC-3-9, from a consortium obtained from the soil of a wheat field known for the occurrence of frequent Fusarium head blight epidemics under aerobic conditions. Along with MMYPF media, a combination of two antibiotics (sulfadiazine and trimethoprim) substantially increased the relative abundance of Desulfitobacterium species from 1.55% (aerobic) to 29.11% (aerobic) and 28.63% (anaerobic). A single colony purified strain, PGC-3-9, was isolated and a 16S rRNA sequencing analysis determined that it was Desulfitobacterium. The PGC-3-9 strain completely de-epoxidated HT-2, deoxynivalenol (DON), nivalenol and 15-acetyl deoxynivalenol, and efficiently eliminated DON in wheat grains under aerobic and anaerobic conditions. The strain PGC-3-9 exhibited high DON de-epoxidation activity at a wide range of pH (6–10) and temperature (15–50 °C) values under both conditions. This strain may be used for the development of detoxification agents in the agriculture and feed industries and the isolation of de-epoxidation enzymes.
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spelling pubmed-73544942020-08-05 Novel Soil Bacterium Strain Desulfitobacterium sp. PGC-3-9 Detoxifies Trichothecene Mycotoxins in Wheat via De-Epoxidation under Aerobic and Anaerobic Conditions He, Wei-Jie Shi, Meng-Meng Yang, Peng Huang, Tao Yuan, Qing-Song Yi, Shu-Yuan Wu, Ai-Bo Li, He-Ping Gao, Chun-Bao Zhang, Jing-Bo Liao, Yu-Cai Toxins (Basel) Article Trichothecenes are the most common mycotoxins contaminating small grain cereals worldwide. The C12,13 epoxide group in the trichothecenes was identified as a toxic group posing harm to humans, farm animals, and plants. Aerobic biological de-epoxidation is considered the ideal method of controlling these types of mycotoxins. In this study, we isolated a novel trichothecene mycotoxin-de-epoxidating bacterium, Desulfitobacterium sp. PGC-3-9, from a consortium obtained from the soil of a wheat field known for the occurrence of frequent Fusarium head blight epidemics under aerobic conditions. Along with MMYPF media, a combination of two antibiotics (sulfadiazine and trimethoprim) substantially increased the relative abundance of Desulfitobacterium species from 1.55% (aerobic) to 29.11% (aerobic) and 28.63% (anaerobic). A single colony purified strain, PGC-3-9, was isolated and a 16S rRNA sequencing analysis determined that it was Desulfitobacterium. The PGC-3-9 strain completely de-epoxidated HT-2, deoxynivalenol (DON), nivalenol and 15-acetyl deoxynivalenol, and efficiently eliminated DON in wheat grains under aerobic and anaerobic conditions. The strain PGC-3-9 exhibited high DON de-epoxidation activity at a wide range of pH (6–10) and temperature (15–50 °C) values under both conditions. This strain may be used for the development of detoxification agents in the agriculture and feed industries and the isolation of de-epoxidation enzymes. MDPI 2020-06-01 /pmc/articles/PMC7354494/ /pubmed/32492959 http://dx.doi.org/10.3390/toxins12060363 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
He, Wei-Jie
Shi, Meng-Meng
Yang, Peng
Huang, Tao
Yuan, Qing-Song
Yi, Shu-Yuan
Wu, Ai-Bo
Li, He-Ping
Gao, Chun-Bao
Zhang, Jing-Bo
Liao, Yu-Cai
Novel Soil Bacterium Strain Desulfitobacterium sp. PGC-3-9 Detoxifies Trichothecene Mycotoxins in Wheat via De-Epoxidation under Aerobic and Anaerobic Conditions
title Novel Soil Bacterium Strain Desulfitobacterium sp. PGC-3-9 Detoxifies Trichothecene Mycotoxins in Wheat via De-Epoxidation under Aerobic and Anaerobic Conditions
title_full Novel Soil Bacterium Strain Desulfitobacterium sp. PGC-3-9 Detoxifies Trichothecene Mycotoxins in Wheat via De-Epoxidation under Aerobic and Anaerobic Conditions
title_fullStr Novel Soil Bacterium Strain Desulfitobacterium sp. PGC-3-9 Detoxifies Trichothecene Mycotoxins in Wheat via De-Epoxidation under Aerobic and Anaerobic Conditions
title_full_unstemmed Novel Soil Bacterium Strain Desulfitobacterium sp. PGC-3-9 Detoxifies Trichothecene Mycotoxins in Wheat via De-Epoxidation under Aerobic and Anaerobic Conditions
title_short Novel Soil Bacterium Strain Desulfitobacterium sp. PGC-3-9 Detoxifies Trichothecene Mycotoxins in Wheat via De-Epoxidation under Aerobic and Anaerobic Conditions
title_sort novel soil bacterium strain desulfitobacterium sp. pgc-3-9 detoxifies trichothecene mycotoxins in wheat via de-epoxidation under aerobic and anaerobic conditions
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7354494/
https://www.ncbi.nlm.nih.gov/pubmed/32492959
http://dx.doi.org/10.3390/toxins12060363
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