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Treatment with Class A CpG Oligodeoxynucleotides in Cats with Naturally Occurring Feline Parvovirus Infection: A Prospective Study
Feline parvovirus (FPV) causes severe gastroenteritis and leukopenia in cats; the outcome is poor. Information regarding specific treatments is lacking. Class A CpG oligodeoxynucleotides (CpG-A) are short single-stranded DNAs, stimulating type I interferon production. In cats, CpG-A induced an antiv...
Autores principales: | , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7354499/ https://www.ncbi.nlm.nih.gov/pubmed/32545689 http://dx.doi.org/10.3390/v12060640 |
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author | Ferri, Filippo Porporato, Federico Rossi, Francesco Enache, Daniela Callegari, Carolina Gerardi, Gabriele Coppola, Luigi M. Contiero, Barbara Crinò, Chiara Kohan, Neda Ranjbar Meli, Marina L. Lutz, Hans Hofmann-Lehmann, Regina Zini, Eric |
author_facet | Ferri, Filippo Porporato, Federico Rossi, Francesco Enache, Daniela Callegari, Carolina Gerardi, Gabriele Coppola, Luigi M. Contiero, Barbara Crinò, Chiara Kohan, Neda Ranjbar Meli, Marina L. Lutz, Hans Hofmann-Lehmann, Regina Zini, Eric |
author_sort | Ferri, Filippo |
collection | PubMed |
description | Feline parvovirus (FPV) causes severe gastroenteritis and leukopenia in cats; the outcome is poor. Information regarding specific treatments is lacking. Class A CpG oligodeoxynucleotides (CpG-A) are short single-stranded DNAs, stimulating type I interferon production. In cats, CpG-A induced an antiviral response in vivo and inhibited FPV replication in vitro. The aim was to prospectively investigate the effects of CpG-A on survival, clinical score, hematological findings, antiviral response (cytokines), viremia, and fecal shedding (real-time qPCR) in cats naturally infected with FPV. Forty-two FPV-infected cats were randomized to receive 100 µg/kg of CpG-A (n = 22) or placebo (n = 20) subcutaneously, on admission and after 48 h. Blood and fecal samples were collected on admission, after 1, 3, and 7 days. All 22 cats showed short duration pain during CpG-A injections. The survival rate, clinical score, leukocyte and erythrocyte counts, viremia, and fecal shedding at any time-point did not differ between cats treated with CpG-A (50%) and placebo (40%). Antiviral myxovirus resistance (Mx) gene transcription increased in both groups from day 1 to 3 (p = 0.005). Antibodies against FPV on admission were associated with survival in cats (p = 0.002). In conclusion, CpG-A treatment did not improve the outcome in cats with FPV infection. FPV infection produced an antiviral response. |
format | Online Article Text |
id | pubmed-7354499 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-73544992020-08-05 Treatment with Class A CpG Oligodeoxynucleotides in Cats with Naturally Occurring Feline Parvovirus Infection: A Prospective Study Ferri, Filippo Porporato, Federico Rossi, Francesco Enache, Daniela Callegari, Carolina Gerardi, Gabriele Coppola, Luigi M. Contiero, Barbara Crinò, Chiara Kohan, Neda Ranjbar Meli, Marina L. Lutz, Hans Hofmann-Lehmann, Regina Zini, Eric Viruses Article Feline parvovirus (FPV) causes severe gastroenteritis and leukopenia in cats; the outcome is poor. Information regarding specific treatments is lacking. Class A CpG oligodeoxynucleotides (CpG-A) are short single-stranded DNAs, stimulating type I interferon production. In cats, CpG-A induced an antiviral response in vivo and inhibited FPV replication in vitro. The aim was to prospectively investigate the effects of CpG-A on survival, clinical score, hematological findings, antiviral response (cytokines), viremia, and fecal shedding (real-time qPCR) in cats naturally infected with FPV. Forty-two FPV-infected cats were randomized to receive 100 µg/kg of CpG-A (n = 22) or placebo (n = 20) subcutaneously, on admission and after 48 h. Blood and fecal samples were collected on admission, after 1, 3, and 7 days. All 22 cats showed short duration pain during CpG-A injections. The survival rate, clinical score, leukocyte and erythrocyte counts, viremia, and fecal shedding at any time-point did not differ between cats treated with CpG-A (50%) and placebo (40%). Antiviral myxovirus resistance (Mx) gene transcription increased in both groups from day 1 to 3 (p = 0.005). Antibodies against FPV on admission were associated with survival in cats (p = 0.002). In conclusion, CpG-A treatment did not improve the outcome in cats with FPV infection. FPV infection produced an antiviral response. MDPI 2020-06-12 /pmc/articles/PMC7354499/ /pubmed/32545689 http://dx.doi.org/10.3390/v12060640 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Ferri, Filippo Porporato, Federico Rossi, Francesco Enache, Daniela Callegari, Carolina Gerardi, Gabriele Coppola, Luigi M. Contiero, Barbara Crinò, Chiara Kohan, Neda Ranjbar Meli, Marina L. Lutz, Hans Hofmann-Lehmann, Regina Zini, Eric Treatment with Class A CpG Oligodeoxynucleotides in Cats with Naturally Occurring Feline Parvovirus Infection: A Prospective Study |
title | Treatment with Class A CpG Oligodeoxynucleotides in Cats with Naturally Occurring Feline Parvovirus Infection: A Prospective Study |
title_full | Treatment with Class A CpG Oligodeoxynucleotides in Cats with Naturally Occurring Feline Parvovirus Infection: A Prospective Study |
title_fullStr | Treatment with Class A CpG Oligodeoxynucleotides in Cats with Naturally Occurring Feline Parvovirus Infection: A Prospective Study |
title_full_unstemmed | Treatment with Class A CpG Oligodeoxynucleotides in Cats with Naturally Occurring Feline Parvovirus Infection: A Prospective Study |
title_short | Treatment with Class A CpG Oligodeoxynucleotides in Cats with Naturally Occurring Feline Parvovirus Infection: A Prospective Study |
title_sort | treatment with class a cpg oligodeoxynucleotides in cats with naturally occurring feline parvovirus infection: a prospective study |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7354499/ https://www.ncbi.nlm.nih.gov/pubmed/32545689 http://dx.doi.org/10.3390/v12060640 |
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