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Treatment with Class A CpG Oligodeoxynucleotides in Cats with Naturally Occurring Feline Parvovirus Infection: A Prospective Study

Feline parvovirus (FPV) causes severe gastroenteritis and leukopenia in cats; the outcome is poor. Information regarding specific treatments is lacking. Class A CpG oligodeoxynucleotides (CpG-A) are short single-stranded DNAs, stimulating type I interferon production. In cats, CpG-A induced an antiv...

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Autores principales: Ferri, Filippo, Porporato, Federico, Rossi, Francesco, Enache, Daniela, Callegari, Carolina, Gerardi, Gabriele, Coppola, Luigi M., Contiero, Barbara, Crinò, Chiara, Kohan, Neda Ranjbar, Meli, Marina L., Lutz, Hans, Hofmann-Lehmann, Regina, Zini, Eric
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7354499/
https://www.ncbi.nlm.nih.gov/pubmed/32545689
http://dx.doi.org/10.3390/v12060640
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author Ferri, Filippo
Porporato, Federico
Rossi, Francesco
Enache, Daniela
Callegari, Carolina
Gerardi, Gabriele
Coppola, Luigi M.
Contiero, Barbara
Crinò, Chiara
Kohan, Neda Ranjbar
Meli, Marina L.
Lutz, Hans
Hofmann-Lehmann, Regina
Zini, Eric
author_facet Ferri, Filippo
Porporato, Federico
Rossi, Francesco
Enache, Daniela
Callegari, Carolina
Gerardi, Gabriele
Coppola, Luigi M.
Contiero, Barbara
Crinò, Chiara
Kohan, Neda Ranjbar
Meli, Marina L.
Lutz, Hans
Hofmann-Lehmann, Regina
Zini, Eric
author_sort Ferri, Filippo
collection PubMed
description Feline parvovirus (FPV) causes severe gastroenteritis and leukopenia in cats; the outcome is poor. Information regarding specific treatments is lacking. Class A CpG oligodeoxynucleotides (CpG-A) are short single-stranded DNAs, stimulating type I interferon production. In cats, CpG-A induced an antiviral response in vivo and inhibited FPV replication in vitro. The aim was to prospectively investigate the effects of CpG-A on survival, clinical score, hematological findings, antiviral response (cytokines), viremia, and fecal shedding (real-time qPCR) in cats naturally infected with FPV. Forty-two FPV-infected cats were randomized to receive 100 µg/kg of CpG-A (n = 22) or placebo (n = 20) subcutaneously, on admission and after 48 h. Blood and fecal samples were collected on admission, after 1, 3, and 7 days. All 22 cats showed short duration pain during CpG-A injections. The survival rate, clinical score, leukocyte and erythrocyte counts, viremia, and fecal shedding at any time-point did not differ between cats treated with CpG-A (50%) and placebo (40%). Antiviral myxovirus resistance (Mx) gene transcription increased in both groups from day 1 to 3 (p = 0.005). Antibodies against FPV on admission were associated with survival in cats (p = 0.002). In conclusion, CpG-A treatment did not improve the outcome in cats with FPV infection. FPV infection produced an antiviral response.
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spelling pubmed-73544992020-08-05 Treatment with Class A CpG Oligodeoxynucleotides in Cats with Naturally Occurring Feline Parvovirus Infection: A Prospective Study Ferri, Filippo Porporato, Federico Rossi, Francesco Enache, Daniela Callegari, Carolina Gerardi, Gabriele Coppola, Luigi M. Contiero, Barbara Crinò, Chiara Kohan, Neda Ranjbar Meli, Marina L. Lutz, Hans Hofmann-Lehmann, Regina Zini, Eric Viruses Article Feline parvovirus (FPV) causes severe gastroenteritis and leukopenia in cats; the outcome is poor. Information regarding specific treatments is lacking. Class A CpG oligodeoxynucleotides (CpG-A) are short single-stranded DNAs, stimulating type I interferon production. In cats, CpG-A induced an antiviral response in vivo and inhibited FPV replication in vitro. The aim was to prospectively investigate the effects of CpG-A on survival, clinical score, hematological findings, antiviral response (cytokines), viremia, and fecal shedding (real-time qPCR) in cats naturally infected with FPV. Forty-two FPV-infected cats were randomized to receive 100 µg/kg of CpG-A (n = 22) or placebo (n = 20) subcutaneously, on admission and after 48 h. Blood and fecal samples were collected on admission, after 1, 3, and 7 days. All 22 cats showed short duration pain during CpG-A injections. The survival rate, clinical score, leukocyte and erythrocyte counts, viremia, and fecal shedding at any time-point did not differ between cats treated with CpG-A (50%) and placebo (40%). Antiviral myxovirus resistance (Mx) gene transcription increased in both groups from day 1 to 3 (p = 0.005). Antibodies against FPV on admission were associated with survival in cats (p = 0.002). In conclusion, CpG-A treatment did not improve the outcome in cats with FPV infection. FPV infection produced an antiviral response. MDPI 2020-06-12 /pmc/articles/PMC7354499/ /pubmed/32545689 http://dx.doi.org/10.3390/v12060640 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Ferri, Filippo
Porporato, Federico
Rossi, Francesco
Enache, Daniela
Callegari, Carolina
Gerardi, Gabriele
Coppola, Luigi M.
Contiero, Barbara
Crinò, Chiara
Kohan, Neda Ranjbar
Meli, Marina L.
Lutz, Hans
Hofmann-Lehmann, Regina
Zini, Eric
Treatment with Class A CpG Oligodeoxynucleotides in Cats with Naturally Occurring Feline Parvovirus Infection: A Prospective Study
title Treatment with Class A CpG Oligodeoxynucleotides in Cats with Naturally Occurring Feline Parvovirus Infection: A Prospective Study
title_full Treatment with Class A CpG Oligodeoxynucleotides in Cats with Naturally Occurring Feline Parvovirus Infection: A Prospective Study
title_fullStr Treatment with Class A CpG Oligodeoxynucleotides in Cats with Naturally Occurring Feline Parvovirus Infection: A Prospective Study
title_full_unstemmed Treatment with Class A CpG Oligodeoxynucleotides in Cats with Naturally Occurring Feline Parvovirus Infection: A Prospective Study
title_short Treatment with Class A CpG Oligodeoxynucleotides in Cats with Naturally Occurring Feline Parvovirus Infection: A Prospective Study
title_sort treatment with class a cpg oligodeoxynucleotides in cats with naturally occurring feline parvovirus infection: a prospective study
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7354499/
https://www.ncbi.nlm.nih.gov/pubmed/32545689
http://dx.doi.org/10.3390/v12060640
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