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Metabolic Traits of Bovine Shiga Toxin-Producing Escherichia coli (STEC) Strains with Different Colonization Properties

Cattle harbor Shiga toxin-producing Escherichia coli (STEC) in their intestinal tract, thereby providing these microorganisms with an ecological niche, but without this colonization leading to any clinical signs. In a preceding study, genotypic characterization of bovine STEC isolates unveiled that...

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Autores principales: Barth, Stefanie A., Weber, Michael, Schaufler, Katharina, Berens, Christian, Geue, Lutz, Menge, Christian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7354573/
https://www.ncbi.nlm.nih.gov/pubmed/32580365
http://dx.doi.org/10.3390/toxins12060414
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author Barth, Stefanie A.
Weber, Michael
Schaufler, Katharina
Berens, Christian
Geue, Lutz
Menge, Christian
author_facet Barth, Stefanie A.
Weber, Michael
Schaufler, Katharina
Berens, Christian
Geue, Lutz
Menge, Christian
author_sort Barth, Stefanie A.
collection PubMed
description Cattle harbor Shiga toxin-producing Escherichia coli (STEC) in their intestinal tract, thereby providing these microorganisms with an ecological niche, but without this colonization leading to any clinical signs. In a preceding study, genotypic characterization of bovine STEC isolates unveiled that their ability to colonize cattle persistently (STEC(per)) or only sporadically (STEC(spo)) is more closely associated with the overall composition of the accessory rather than the core genome. However, the colonization pattern could not be unequivocally linked to the possession of classical virulence genes. This study aimed at assessing, therefore, if the presence of certain phenotypic traits in the strains determines their colonization pattern and if these can be traced back to distinctive genetic features. STEC(spo) strains produced significantly more biofilm than STEC(per) when incubated at lower temperatures. Key substrates, the metabolism of which showed a significant association with colonization type, were glyoxylic acid and L-rhamnose, which were utilized by STEC(spo), but not or only by some STEC(per). Genomic sequences of the respective glc and rha operons contained mutations and frameshifts in uptake and/or regulatory genes, particularly in STEC(per). These findings suggest that STEC(spo) conserved features leveraging survival in the environment, whereas the acquisition of a persistent colonization phenotype in the cattle reservoir was accompanied by the loss of metabolic properties and genomic mutations in the underlying genetic pathways.
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spelling pubmed-73545732020-07-23 Metabolic Traits of Bovine Shiga Toxin-Producing Escherichia coli (STEC) Strains with Different Colonization Properties Barth, Stefanie A. Weber, Michael Schaufler, Katharina Berens, Christian Geue, Lutz Menge, Christian Toxins (Basel) Article Cattle harbor Shiga toxin-producing Escherichia coli (STEC) in their intestinal tract, thereby providing these microorganisms with an ecological niche, but without this colonization leading to any clinical signs. In a preceding study, genotypic characterization of bovine STEC isolates unveiled that their ability to colonize cattle persistently (STEC(per)) or only sporadically (STEC(spo)) is more closely associated with the overall composition of the accessory rather than the core genome. However, the colonization pattern could not be unequivocally linked to the possession of classical virulence genes. This study aimed at assessing, therefore, if the presence of certain phenotypic traits in the strains determines their colonization pattern and if these can be traced back to distinctive genetic features. STEC(spo) strains produced significantly more biofilm than STEC(per) when incubated at lower temperatures. Key substrates, the metabolism of which showed a significant association with colonization type, were glyoxylic acid and L-rhamnose, which were utilized by STEC(spo), but not or only by some STEC(per). Genomic sequences of the respective glc and rha operons contained mutations and frameshifts in uptake and/or regulatory genes, particularly in STEC(per). These findings suggest that STEC(spo) conserved features leveraging survival in the environment, whereas the acquisition of a persistent colonization phenotype in the cattle reservoir was accompanied by the loss of metabolic properties and genomic mutations in the underlying genetic pathways. MDPI 2020-06-22 /pmc/articles/PMC7354573/ /pubmed/32580365 http://dx.doi.org/10.3390/toxins12060414 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Barth, Stefanie A.
Weber, Michael
Schaufler, Katharina
Berens, Christian
Geue, Lutz
Menge, Christian
Metabolic Traits of Bovine Shiga Toxin-Producing Escherichia coli (STEC) Strains with Different Colonization Properties
title Metabolic Traits of Bovine Shiga Toxin-Producing Escherichia coli (STEC) Strains with Different Colonization Properties
title_full Metabolic Traits of Bovine Shiga Toxin-Producing Escherichia coli (STEC) Strains with Different Colonization Properties
title_fullStr Metabolic Traits of Bovine Shiga Toxin-Producing Escherichia coli (STEC) Strains with Different Colonization Properties
title_full_unstemmed Metabolic Traits of Bovine Shiga Toxin-Producing Escherichia coli (STEC) Strains with Different Colonization Properties
title_short Metabolic Traits of Bovine Shiga Toxin-Producing Escherichia coli (STEC) Strains with Different Colonization Properties
title_sort metabolic traits of bovine shiga toxin-producing escherichia coli (stec) strains with different colonization properties
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7354573/
https://www.ncbi.nlm.nih.gov/pubmed/32580365
http://dx.doi.org/10.3390/toxins12060414
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