Absence of Cyanotoxins in Llayta, Edible Nostocaceae Colonies from the Andes Highlands

Edible Llayta are cyanobacterial colonies consumed in the Andes highlands. Llayta and four isolated cyanobacteria strains were tested for cyanotoxins (microcystin, nodularin, cylindrospermopsin, saxitoxin and β-N-methylamino-L-alanine—BMAA) using molecular and chemical methods. All isolates were fre...

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Autores principales: Galetović, Alexandra, Azevedo, Joana, Castelo-Branco, Raquel, Oliveira, Flavio, Gómez-Silva, Benito, Vasconcelos, Vitor
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7354591/
https://www.ncbi.nlm.nih.gov/pubmed/32526918
http://dx.doi.org/10.3390/toxins12060382
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author Galetović, Alexandra
Azevedo, Joana
Castelo-Branco, Raquel
Oliveira, Flavio
Gómez-Silva, Benito
Vasconcelos, Vitor
author_facet Galetović, Alexandra
Azevedo, Joana
Castelo-Branco, Raquel
Oliveira, Flavio
Gómez-Silva, Benito
Vasconcelos, Vitor
author_sort Galetović, Alexandra
collection PubMed
description Edible Llayta are cyanobacterial colonies consumed in the Andes highlands. Llayta and four isolated cyanobacteria strains were tested for cyanotoxins (microcystin, nodularin, cylindrospermopsin, saxitoxin and β-N-methylamino-L-alanine—BMAA) using molecular and chemical methods. All isolates were free of target genes involved in toxin biosynthesis. Only DNA from Llayta amplified the mcyE gene. Presence of microcystin-LR and BMAA in Llayta extracts was discarded by LC/MS analyses. The analysed Llayta colonies have an incomplete microcystin biosynthetic pathway and are a safe food ingredient.
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spelling pubmed-73545912020-07-23 Absence of Cyanotoxins in Llayta, Edible Nostocaceae Colonies from the Andes Highlands Galetović, Alexandra Azevedo, Joana Castelo-Branco, Raquel Oliveira, Flavio Gómez-Silva, Benito Vasconcelos, Vitor Toxins (Basel) Article Edible Llayta are cyanobacterial colonies consumed in the Andes highlands. Llayta and four isolated cyanobacteria strains were tested for cyanotoxins (microcystin, nodularin, cylindrospermopsin, saxitoxin and β-N-methylamino-L-alanine—BMAA) using molecular and chemical methods. All isolates were free of target genes involved in toxin biosynthesis. Only DNA from Llayta amplified the mcyE gene. Presence of microcystin-LR and BMAA in Llayta extracts was discarded by LC/MS analyses. The analysed Llayta colonies have an incomplete microcystin biosynthetic pathway and are a safe food ingredient. MDPI 2020-06-09 /pmc/articles/PMC7354591/ /pubmed/32526918 http://dx.doi.org/10.3390/toxins12060382 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Galetović, Alexandra
Azevedo, Joana
Castelo-Branco, Raquel
Oliveira, Flavio
Gómez-Silva, Benito
Vasconcelos, Vitor
Absence of Cyanotoxins in Llayta, Edible Nostocaceae Colonies from the Andes Highlands
title Absence of Cyanotoxins in Llayta, Edible Nostocaceae Colonies from the Andes Highlands
title_full Absence of Cyanotoxins in Llayta, Edible Nostocaceae Colonies from the Andes Highlands
title_fullStr Absence of Cyanotoxins in Llayta, Edible Nostocaceae Colonies from the Andes Highlands
title_full_unstemmed Absence of Cyanotoxins in Llayta, Edible Nostocaceae Colonies from the Andes Highlands
title_short Absence of Cyanotoxins in Llayta, Edible Nostocaceae Colonies from the Andes Highlands
title_sort absence of cyanotoxins in llayta, edible nostocaceae colonies from the andes highlands
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7354591/
https://www.ncbi.nlm.nih.gov/pubmed/32526918
http://dx.doi.org/10.3390/toxins12060382
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