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Reporter Replicons for Antiviral Drug Discovery against Positive Single-Stranded RNA Viruses

Single-stranded positive RNA ((+) ssRNA) viruses include several important human pathogens. Some members are responsible for large outbreaks, such as Zika virus, West Nile virus, SARS-CoV, and SARS-CoV-2, while others are endemic, causing an enormous global health burden. Since vaccines or specific...

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Autores principales: Fernandes, Rafaela S., Freire, Marjorie C. L. C., Bueno, Renata V., Godoy, Andre S., Gil, Laura H. V. G., Oliva, Glaucius
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7354593/
https://www.ncbi.nlm.nih.gov/pubmed/32486283
http://dx.doi.org/10.3390/v12060598
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author Fernandes, Rafaela S.
Freire, Marjorie C. L. C.
Bueno, Renata V.
Godoy, Andre S.
Gil, Laura H. V. G.
Oliva, Glaucius
author_facet Fernandes, Rafaela S.
Freire, Marjorie C. L. C.
Bueno, Renata V.
Godoy, Andre S.
Gil, Laura H. V. G.
Oliva, Glaucius
author_sort Fernandes, Rafaela S.
collection PubMed
description Single-stranded positive RNA ((+) ssRNA) viruses include several important human pathogens. Some members are responsible for large outbreaks, such as Zika virus, West Nile virus, SARS-CoV, and SARS-CoV-2, while others are endemic, causing an enormous global health burden. Since vaccines or specific treatments are not available for most viral infections, the discovery of direct-acting antivirals (DAA) is an urgent need. Still, the low-throughput nature of and biosafety concerns related to traditional antiviral assays hinders the discovery of new inhibitors. With the advances of reverse genetics, reporter replicon systems have become an alternative tool for the screening of DAAs. Herein, we review decades of the use of (+) ssRNA viruses replicon systems for the discovery of antiviral agents. We summarize different strategies used to develop those systems, as well as highlight some of the most promising inhibitors identified by the method. Despite the genetic alterations introduced, reporter replicons have been shown to be reliable systems for screening and identification of viral replication inhibitors and, therefore, an important tool for the discovery of new DAAs.
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spelling pubmed-73545932020-07-23 Reporter Replicons for Antiviral Drug Discovery against Positive Single-Stranded RNA Viruses Fernandes, Rafaela S. Freire, Marjorie C. L. C. Bueno, Renata V. Godoy, Andre S. Gil, Laura H. V. G. Oliva, Glaucius Viruses Review Single-stranded positive RNA ((+) ssRNA) viruses include several important human pathogens. Some members are responsible for large outbreaks, such as Zika virus, West Nile virus, SARS-CoV, and SARS-CoV-2, while others are endemic, causing an enormous global health burden. Since vaccines or specific treatments are not available for most viral infections, the discovery of direct-acting antivirals (DAA) is an urgent need. Still, the low-throughput nature of and biosafety concerns related to traditional antiviral assays hinders the discovery of new inhibitors. With the advances of reverse genetics, reporter replicon systems have become an alternative tool for the screening of DAAs. Herein, we review decades of the use of (+) ssRNA viruses replicon systems for the discovery of antiviral agents. We summarize different strategies used to develop those systems, as well as highlight some of the most promising inhibitors identified by the method. Despite the genetic alterations introduced, reporter replicons have been shown to be reliable systems for screening and identification of viral replication inhibitors and, therefore, an important tool for the discovery of new DAAs. MDPI 2020-05-30 /pmc/articles/PMC7354593/ /pubmed/32486283 http://dx.doi.org/10.3390/v12060598 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Review
Fernandes, Rafaela S.
Freire, Marjorie C. L. C.
Bueno, Renata V.
Godoy, Andre S.
Gil, Laura H. V. G.
Oliva, Glaucius
Reporter Replicons for Antiviral Drug Discovery against Positive Single-Stranded RNA Viruses
title Reporter Replicons for Antiviral Drug Discovery against Positive Single-Stranded RNA Viruses
title_full Reporter Replicons for Antiviral Drug Discovery against Positive Single-Stranded RNA Viruses
title_fullStr Reporter Replicons for Antiviral Drug Discovery against Positive Single-Stranded RNA Viruses
title_full_unstemmed Reporter Replicons for Antiviral Drug Discovery against Positive Single-Stranded RNA Viruses
title_short Reporter Replicons for Antiviral Drug Discovery against Positive Single-Stranded RNA Viruses
title_sort reporter replicons for antiviral drug discovery against positive single-stranded rna viruses
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7354593/
https://www.ncbi.nlm.nih.gov/pubmed/32486283
http://dx.doi.org/10.3390/v12060598
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