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An Observational Study: Is N-Acetylcysteine Helpful in Performance Improvement of Mycoplasma IST2 Testing through Sample Homogenization?

BACKGROUND: Culture is still the gold standard for the detection of genital mycoplasma which could cause urogenital infections in humans. Mycoplasma IST2 is a commercial kit widely used for the detection of M. hominis and Ureaplasma species. Its accuracy was partially impaired because clinical speci...

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Autores principales: Mao, Xin-Ru, Wang, Rui-Cheng, Li, Rong-Jiao, Zhou, Cai-Rong, Chen, Xian-Kai, Cheng, Can-Can, Yin, Xiao-Mao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7354645/
https://www.ncbi.nlm.nih.gov/pubmed/32714477
http://dx.doi.org/10.1155/2020/1391698
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author Mao, Xin-Ru
Wang, Rui-Cheng
Li, Rong-Jiao
Zhou, Cai-Rong
Chen, Xian-Kai
Cheng, Can-Can
Yin, Xiao-Mao
author_facet Mao, Xin-Ru
Wang, Rui-Cheng
Li, Rong-Jiao
Zhou, Cai-Rong
Chen, Xian-Kai
Cheng, Can-Can
Yin, Xiao-Mao
author_sort Mao, Xin-Ru
collection PubMed
description BACKGROUND: Culture is still the gold standard for the detection of genital mycoplasma which could cause urogenital infections in humans. Mycoplasma IST2 is a commercial kit widely used for the detection of M. hominis and Ureaplasma species. Its accuracy was partially impaired because clinical specimens are usually mixed with purulent or transparent mucus. We aimed to solve this problem through sample homogenization by N-acetylcysteine (NAC) treatment. METHODS: Twenty-two endocervical swab samples were collected from 22 female patients with suspected mycoplasma infection, while 11 of these specimens were with purulent or transparent mucus. Mycoplasma IST2 testing kit was used for mycoplasma culture and AST for the control group and NAC-treated group. RESULTS: Genital mycoplasma was detected in 15 of 22 samples for both groups. The colony number in 6 out of 11 purulent specimens (54.5%) was more than 10(4) CFU/ml of genital mycoplasma for the NAC-treated group, while only one of 11 (9.1%) for the control group. For the nonpurulent specimens, no significant difference had been found in colony counting of genital mycoplasma between the control group and NAC-treated group (P > 0.05). The results of antimicrobial susceptibility testing for the NAC-treated group were highly similar to those for the control group. CONCLUSIONS: Our results demonstrate that NAC is helpful in sample homogenization and NAC treatment can improve the detection efficiency of mycoplasma with Mycoplasma IST2 testing.
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spelling pubmed-73546452020-07-23 An Observational Study: Is N-Acetylcysteine Helpful in Performance Improvement of Mycoplasma IST2 Testing through Sample Homogenization? Mao, Xin-Ru Wang, Rui-Cheng Li, Rong-Jiao Zhou, Cai-Rong Chen, Xian-Kai Cheng, Can-Can Yin, Xiao-Mao Can J Infect Dis Med Microbiol Research Article BACKGROUND: Culture is still the gold standard for the detection of genital mycoplasma which could cause urogenital infections in humans. Mycoplasma IST2 is a commercial kit widely used for the detection of M. hominis and Ureaplasma species. Its accuracy was partially impaired because clinical specimens are usually mixed with purulent or transparent mucus. We aimed to solve this problem through sample homogenization by N-acetylcysteine (NAC) treatment. METHODS: Twenty-two endocervical swab samples were collected from 22 female patients with suspected mycoplasma infection, while 11 of these specimens were with purulent or transparent mucus. Mycoplasma IST2 testing kit was used for mycoplasma culture and AST for the control group and NAC-treated group. RESULTS: Genital mycoplasma was detected in 15 of 22 samples for both groups. The colony number in 6 out of 11 purulent specimens (54.5%) was more than 10(4) CFU/ml of genital mycoplasma for the NAC-treated group, while only one of 11 (9.1%) for the control group. For the nonpurulent specimens, no significant difference had been found in colony counting of genital mycoplasma between the control group and NAC-treated group (P > 0.05). The results of antimicrobial susceptibility testing for the NAC-treated group were highly similar to those for the control group. CONCLUSIONS: Our results demonstrate that NAC is helpful in sample homogenization and NAC treatment can improve the detection efficiency of mycoplasma with Mycoplasma IST2 testing. Hindawi 2020-07-02 /pmc/articles/PMC7354645/ /pubmed/32714477 http://dx.doi.org/10.1155/2020/1391698 Text en Copyright © 2020 Xin-Ru Mao et al. http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Mao, Xin-Ru
Wang, Rui-Cheng
Li, Rong-Jiao
Zhou, Cai-Rong
Chen, Xian-Kai
Cheng, Can-Can
Yin, Xiao-Mao
An Observational Study: Is N-Acetylcysteine Helpful in Performance Improvement of Mycoplasma IST2 Testing through Sample Homogenization?
title An Observational Study: Is N-Acetylcysteine Helpful in Performance Improvement of Mycoplasma IST2 Testing through Sample Homogenization?
title_full An Observational Study: Is N-Acetylcysteine Helpful in Performance Improvement of Mycoplasma IST2 Testing through Sample Homogenization?
title_fullStr An Observational Study: Is N-Acetylcysteine Helpful in Performance Improvement of Mycoplasma IST2 Testing through Sample Homogenization?
title_full_unstemmed An Observational Study: Is N-Acetylcysteine Helpful in Performance Improvement of Mycoplasma IST2 Testing through Sample Homogenization?
title_short An Observational Study: Is N-Acetylcysteine Helpful in Performance Improvement of Mycoplasma IST2 Testing through Sample Homogenization?
title_sort observational study: is n-acetylcysteine helpful in performance improvement of mycoplasma ist2 testing through sample homogenization?
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7354645/
https://www.ncbi.nlm.nih.gov/pubmed/32714477
http://dx.doi.org/10.1155/2020/1391698
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