Cargando…
Flexible, diamond-based microelectrodes fabricated using the diamond growth side for neural sensing
Diamond possesses many favorable properties for biochemical sensors, including biocompatibility, chemical inertness, resistance to biofouling, an extremely wide potential window, and low double-layer capacitance. The hardness of diamond, however, has hindered its applications in neural implants due...
Autores principales: | , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2020
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7355183/ https://www.ncbi.nlm.nih.gov/pubmed/32685185 http://dx.doi.org/10.1038/s41378-020-0155-1 |
_version_ | 1783558224686874624 |
---|---|
author | Fan, Bin Rusinek, Cory A. Thompson, Cort H. Setien, Monica Guo, Yue Rechenberg, Robert Gong, Yan Weber, Arthur J. Becker, Michael F. Purcell, Erin Li, Wen |
author_facet | Fan, Bin Rusinek, Cory A. Thompson, Cort H. Setien, Monica Guo, Yue Rechenberg, Robert Gong, Yan Weber, Arthur J. Becker, Michael F. Purcell, Erin Li, Wen |
author_sort | Fan, Bin |
collection | PubMed |
description | Diamond possesses many favorable properties for biochemical sensors, including biocompatibility, chemical inertness, resistance to biofouling, an extremely wide potential window, and low double-layer capacitance. The hardness of diamond, however, has hindered its applications in neural implants due to the mechanical property mismatch between diamond and soft nervous tissues. Here, we present a flexible, diamond-based microelectrode probe consisting of multichannel boron-doped polycrystalline diamond (BDD) microelectrodes on a soft Parylene C substrate. We developed and optimized a wafer-scale fabrication approach that allows the use of the growth side of the BDD thin film as the sensing surface. Compared to the nucleation surface, the BDD growth side exhibited a rougher morphology, a higher sp(3) content, a wider water potential window, and a lower background current. The dopamine (DA) sensing capability of the BDD growth surface electrodes was validated in a 1.0 mM DA solution, which shows better sensitivity and stability than the BDD nucleation surface electrodes. The results of these comparative studies suggest that using the BDD growth surface for making implantable microelectrodes has significant advantages in terms of the sensitivity, selectivity, and stability of a neural implant. Furthermore, we validated the functionality of the BDD growth side electrodes for neural recordings both in vitro and in vivo. The biocompatibility of the microcrystalline diamond film was also assessed in vitro using rat cortical neuron cultures. |
format | Online Article Text |
id | pubmed-7355183 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-73551832020-07-16 Flexible, diamond-based microelectrodes fabricated using the diamond growth side for neural sensing Fan, Bin Rusinek, Cory A. Thompson, Cort H. Setien, Monica Guo, Yue Rechenberg, Robert Gong, Yan Weber, Arthur J. Becker, Michael F. Purcell, Erin Li, Wen Microsyst Nanoeng Article Diamond possesses many favorable properties for biochemical sensors, including biocompatibility, chemical inertness, resistance to biofouling, an extremely wide potential window, and low double-layer capacitance. The hardness of diamond, however, has hindered its applications in neural implants due to the mechanical property mismatch between diamond and soft nervous tissues. Here, we present a flexible, diamond-based microelectrode probe consisting of multichannel boron-doped polycrystalline diamond (BDD) microelectrodes on a soft Parylene C substrate. We developed and optimized a wafer-scale fabrication approach that allows the use of the growth side of the BDD thin film as the sensing surface. Compared to the nucleation surface, the BDD growth side exhibited a rougher morphology, a higher sp(3) content, a wider water potential window, and a lower background current. The dopamine (DA) sensing capability of the BDD growth surface electrodes was validated in a 1.0 mM DA solution, which shows better sensitivity and stability than the BDD nucleation surface electrodes. The results of these comparative studies suggest that using the BDD growth surface for making implantable microelectrodes has significant advantages in terms of the sensitivity, selectivity, and stability of a neural implant. Furthermore, we validated the functionality of the BDD growth side electrodes for neural recordings both in vitro and in vivo. The biocompatibility of the microcrystalline diamond film was also assessed in vitro using rat cortical neuron cultures. Nature Publishing Group UK 2020-07-13 /pmc/articles/PMC7355183/ /pubmed/32685185 http://dx.doi.org/10.1038/s41378-020-0155-1 Text en © The Author(s) 2020 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Article Fan, Bin Rusinek, Cory A. Thompson, Cort H. Setien, Monica Guo, Yue Rechenberg, Robert Gong, Yan Weber, Arthur J. Becker, Michael F. Purcell, Erin Li, Wen Flexible, diamond-based microelectrodes fabricated using the diamond growth side for neural sensing |
title | Flexible, diamond-based microelectrodes fabricated using the diamond growth side for neural sensing |
title_full | Flexible, diamond-based microelectrodes fabricated using the diamond growth side for neural sensing |
title_fullStr | Flexible, diamond-based microelectrodes fabricated using the diamond growth side for neural sensing |
title_full_unstemmed | Flexible, diamond-based microelectrodes fabricated using the diamond growth side for neural sensing |
title_short | Flexible, diamond-based microelectrodes fabricated using the diamond growth side for neural sensing |
title_sort | flexible, diamond-based microelectrodes fabricated using the diamond growth side for neural sensing |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7355183/ https://www.ncbi.nlm.nih.gov/pubmed/32685185 http://dx.doi.org/10.1038/s41378-020-0155-1 |
work_keys_str_mv | AT fanbin flexiblediamondbasedmicroelectrodesfabricatedusingthediamondgrowthsideforneuralsensing AT rusinekcorya flexiblediamondbasedmicroelectrodesfabricatedusingthediamondgrowthsideforneuralsensing AT thompsoncorth flexiblediamondbasedmicroelectrodesfabricatedusingthediamondgrowthsideforneuralsensing AT setienmonica flexiblediamondbasedmicroelectrodesfabricatedusingthediamondgrowthsideforneuralsensing AT guoyue flexiblediamondbasedmicroelectrodesfabricatedusingthediamondgrowthsideforneuralsensing AT rechenbergrobert flexiblediamondbasedmicroelectrodesfabricatedusingthediamondgrowthsideforneuralsensing AT gongyan flexiblediamondbasedmicroelectrodesfabricatedusingthediamondgrowthsideforneuralsensing AT weberarthurj flexiblediamondbasedmicroelectrodesfabricatedusingthediamondgrowthsideforneuralsensing AT beckermichaelf flexiblediamondbasedmicroelectrodesfabricatedusingthediamondgrowthsideforneuralsensing AT purcellerin flexiblediamondbasedmicroelectrodesfabricatedusingthediamondgrowthsideforneuralsensing AT liwen flexiblediamondbasedmicroelectrodesfabricatedusingthediamondgrowthsideforneuralsensing |