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Osmotic perception of GABAergic synaptic transmission in the supraoptic nucleus of rats

Extracellular osmolality plays a crucial role in controlling the activation of neurons. Hypertonic stimulation modulates glutamatergic inputs to the supraoptic nucleus (SON) magnocellular neurosecretory cells (MNCs) putative vasopressin (VP) neurons through capsaicin-insensitive transient receptor p...

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Autores principales: Ohbuchi, Toyoaki, Saito, Takeshi, Yokoyama, Toru, Hashimoto, Hirofumi, Maruyama, Takashi, Suzuki, Hideaki, Ueta, Yoichi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7355382/
https://www.ncbi.nlm.nih.gov/pubmed/32685762
http://dx.doi.org/10.1016/j.ibror.2020.06.007
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author Ohbuchi, Toyoaki
Saito, Takeshi
Yokoyama, Toru
Hashimoto, Hirofumi
Maruyama, Takashi
Suzuki, Hideaki
Ueta, Yoichi
author_facet Ohbuchi, Toyoaki
Saito, Takeshi
Yokoyama, Toru
Hashimoto, Hirofumi
Maruyama, Takashi
Suzuki, Hideaki
Ueta, Yoichi
author_sort Ohbuchi, Toyoaki
collection PubMed
description Extracellular osmolality plays a crucial role in controlling the activation of neurons. Hypertonic stimulation modulates glutamatergic inputs to the supraoptic nucleus (SON) magnocellular neurosecretory cells (MNCs) putative vasopressin (VP) neurons through capsaicin-insensitive transient receptor potential vanilloid (TRPV) 1 channels on the presynaptic terminals. However, it remains unclear whether osmotic stimulation modulates GABAergic inputs to VP-secreting neurons within punched-out slices containing only the SON and the perinuclear zone. To answer this question, we studied the effects of various osmotic conditions on the miniature GABAergic postsynaptic currents (mGPSCs) using the whole-cell patch-clamp technique on rat SON putative VP-secreting neurons in small slice preparations. We revealed that incubation in hypertonic solution for 2 h reduced both the frequency and amplitude of the mGPSCs to the SON putative VP neurons, whereas the mGPSCs were unaffected when the external osmolality was changed from isotonic to hypotonic. Of interest, we found that changing from a hypertonic to hypotonic environment increased the frequency of the mGPSCs. This effect was independent of TRPV4. We hypothesize that two coordinated mechanisms may play an important role in the regulation of a wide range of physiological functions of VP.: 1) the modulation of GABA(A) receptor properties by brain-derived neurotrophic factor (BDNF)-induced tyrosine kinase B receptor-mediated signaling under hypertonic conditions, and 2) cell swelling-induced activation of whole-cell anion currents under hypotonic conditions.
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spelling pubmed-73553822020-07-17 Osmotic perception of GABAergic synaptic transmission in the supraoptic nucleus of rats Ohbuchi, Toyoaki Saito, Takeshi Yokoyama, Toru Hashimoto, Hirofumi Maruyama, Takashi Suzuki, Hideaki Ueta, Yoichi IBRO Rep Article Extracellular osmolality plays a crucial role in controlling the activation of neurons. Hypertonic stimulation modulates glutamatergic inputs to the supraoptic nucleus (SON) magnocellular neurosecretory cells (MNCs) putative vasopressin (VP) neurons through capsaicin-insensitive transient receptor potential vanilloid (TRPV) 1 channels on the presynaptic terminals. However, it remains unclear whether osmotic stimulation modulates GABAergic inputs to VP-secreting neurons within punched-out slices containing only the SON and the perinuclear zone. To answer this question, we studied the effects of various osmotic conditions on the miniature GABAergic postsynaptic currents (mGPSCs) using the whole-cell patch-clamp technique on rat SON putative VP-secreting neurons in small slice preparations. We revealed that incubation in hypertonic solution for 2 h reduced both the frequency and amplitude of the mGPSCs to the SON putative VP neurons, whereas the mGPSCs were unaffected when the external osmolality was changed from isotonic to hypotonic. Of interest, we found that changing from a hypertonic to hypotonic environment increased the frequency of the mGPSCs. This effect was independent of TRPV4. We hypothesize that two coordinated mechanisms may play an important role in the regulation of a wide range of physiological functions of VP.: 1) the modulation of GABA(A) receptor properties by brain-derived neurotrophic factor (BDNF)-induced tyrosine kinase B receptor-mediated signaling under hypertonic conditions, and 2) cell swelling-induced activation of whole-cell anion currents under hypotonic conditions. Elsevier 2020-07-02 /pmc/articles/PMC7355382/ /pubmed/32685762 http://dx.doi.org/10.1016/j.ibror.2020.06.007 Text en © 2020 The Author(s) http://creativecommons.org/licenses/by/4.0/ This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Ohbuchi, Toyoaki
Saito, Takeshi
Yokoyama, Toru
Hashimoto, Hirofumi
Maruyama, Takashi
Suzuki, Hideaki
Ueta, Yoichi
Osmotic perception of GABAergic synaptic transmission in the supraoptic nucleus of rats
title Osmotic perception of GABAergic synaptic transmission in the supraoptic nucleus of rats
title_full Osmotic perception of GABAergic synaptic transmission in the supraoptic nucleus of rats
title_fullStr Osmotic perception of GABAergic synaptic transmission in the supraoptic nucleus of rats
title_full_unstemmed Osmotic perception of GABAergic synaptic transmission in the supraoptic nucleus of rats
title_short Osmotic perception of GABAergic synaptic transmission in the supraoptic nucleus of rats
title_sort osmotic perception of gabaergic synaptic transmission in the supraoptic nucleus of rats
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7355382/
https://www.ncbi.nlm.nih.gov/pubmed/32685762
http://dx.doi.org/10.1016/j.ibror.2020.06.007
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