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Deep tissue volumetric optoacoustic tracking of individual circulating tumor cells in an intracardially perfused mouse model

Widespread metastasis is the major cause of death from melanoma and other types of cancer. At present, the dynamic aspects of the metastatic cascade remain enigmatic. The feasibility to track circulating melanoma cells deep within living intact organisms can greatly impact our knowledge on tumor met...

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Autores principales: Deán-Ben, Xosé Luís, Weidenfeld, Ina, Degtyaruk, Oleksiy, Ntziachristos, Vasilis, Stiel, Andre C., Razansky, Daniel
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Neoplasia Press 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7355386/
https://www.ncbi.nlm.nih.gov/pubmed/32653834
http://dx.doi.org/10.1016/j.neo.2020.06.008
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author Deán-Ben, Xosé Luís
Weidenfeld, Ina
Degtyaruk, Oleksiy
Ntziachristos, Vasilis
Stiel, Andre C.
Razansky, Daniel
author_facet Deán-Ben, Xosé Luís
Weidenfeld, Ina
Degtyaruk, Oleksiy
Ntziachristos, Vasilis
Stiel, Andre C.
Razansky, Daniel
author_sort Deán-Ben, Xosé Luís
collection PubMed
description Widespread metastasis is the major cause of death from melanoma and other types of cancer. At present, the dynamic aspects of the metastatic cascade remain enigmatic. The feasibility to track circulating melanoma cells deep within living intact organisms can greatly impact our knowledge on tumor metastasis, but existing imaging approaches lack the sensitivity, spatio-temporal resolution or penetration depth to capture flowing tumor cells over large fields of view within optically-opaque biological tissues. Vast progress with the development of optoacoustic tomography technologies has recently enabled two- and three-dimensional imaging at unprecedented frame rates in the order of hundreds of Hertz, effectively mapping up to a million image voxels within a single volumetric snapshot. Herein, we employ volumetric optoacoustic tomography for real-time visualization of passage and trapping of individual B16 melanoma cells in the whole mouse brain. Detection of individual circulating melanoma cells was facilitated by substituting blood with an artificial cerebrospinal fluid that removes the strong absorption background in the optoacoustic images. The approach can provide new opportunities for studying trafficking and accumulation of metastatic melanoma cells in different organs.
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spelling pubmed-73553862020-07-20 Deep tissue volumetric optoacoustic tracking of individual circulating tumor cells in an intracardially perfused mouse model Deán-Ben, Xosé Luís Weidenfeld, Ina Degtyaruk, Oleksiy Ntziachristos, Vasilis Stiel, Andre C. Razansky, Daniel Neoplasia Original article Widespread metastasis is the major cause of death from melanoma and other types of cancer. At present, the dynamic aspects of the metastatic cascade remain enigmatic. The feasibility to track circulating melanoma cells deep within living intact organisms can greatly impact our knowledge on tumor metastasis, but existing imaging approaches lack the sensitivity, spatio-temporal resolution or penetration depth to capture flowing tumor cells over large fields of view within optically-opaque biological tissues. Vast progress with the development of optoacoustic tomography technologies has recently enabled two- and three-dimensional imaging at unprecedented frame rates in the order of hundreds of Hertz, effectively mapping up to a million image voxels within a single volumetric snapshot. Herein, we employ volumetric optoacoustic tomography for real-time visualization of passage and trapping of individual B16 melanoma cells in the whole mouse brain. Detection of individual circulating melanoma cells was facilitated by substituting blood with an artificial cerebrospinal fluid that removes the strong absorption background in the optoacoustic images. The approach can provide new opportunities for studying trafficking and accumulation of metastatic melanoma cells in different organs. Neoplasia Press 2020-07-09 /pmc/articles/PMC7355386/ /pubmed/32653834 http://dx.doi.org/10.1016/j.neo.2020.06.008 Text en © 2020 The Authors http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Original article
Deán-Ben, Xosé Luís
Weidenfeld, Ina
Degtyaruk, Oleksiy
Ntziachristos, Vasilis
Stiel, Andre C.
Razansky, Daniel
Deep tissue volumetric optoacoustic tracking of individual circulating tumor cells in an intracardially perfused mouse model
title Deep tissue volumetric optoacoustic tracking of individual circulating tumor cells in an intracardially perfused mouse model
title_full Deep tissue volumetric optoacoustic tracking of individual circulating tumor cells in an intracardially perfused mouse model
title_fullStr Deep tissue volumetric optoacoustic tracking of individual circulating tumor cells in an intracardially perfused mouse model
title_full_unstemmed Deep tissue volumetric optoacoustic tracking of individual circulating tumor cells in an intracardially perfused mouse model
title_short Deep tissue volumetric optoacoustic tracking of individual circulating tumor cells in an intracardially perfused mouse model
title_sort deep tissue volumetric optoacoustic tracking of individual circulating tumor cells in an intracardially perfused mouse model
topic Original article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7355386/
https://www.ncbi.nlm.nih.gov/pubmed/32653834
http://dx.doi.org/10.1016/j.neo.2020.06.008
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