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Imunofan—RDKVYR Peptide—Stimulates Skin Cell Proliferation and Promotes Tissue Repair
Regeneration and wound healing are vital to tissue homeostasis and organism survival. One of the biggest challenges of today’s science and medicine is finding methods and factors to stimulate these processes in the human body. Effective solutions to promote regenerative responses will accelerate adv...
Autores principales: | , , , , , , , , , , , , , , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7355430/ https://www.ncbi.nlm.nih.gov/pubmed/32585846 http://dx.doi.org/10.3390/molecules25122884 |
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author | Sawicka, Justyna Dzierżyńska, Maria Wardowska, Anna Deptuła, Milena Rogujski, Piotr Sosnowski, Paweł Filipowicz, Natalia Mieczkowska, Alina Sass, Piotr Pawlik, Anna Hać, Aleksandra Schumacher, Adriana Gucwa, Magdalena Karska, Natalia Kamińska, Jolanta Płatek, Rafał Mazuryk, Jarosław Zieliński, Jacek Kondej, Karolina Młynarz, Piotr Mucha, Piotr Skowron, Piotr Janus, Łukasz Herman-Antosiewicz, Anna Sachadyn, Paweł Czupryn, Artur Piotrowski, Arkadiusz Pikuła, Michał Rodziewicz-Motowidło, Sylwia |
author_facet | Sawicka, Justyna Dzierżyńska, Maria Wardowska, Anna Deptuła, Milena Rogujski, Piotr Sosnowski, Paweł Filipowicz, Natalia Mieczkowska, Alina Sass, Piotr Pawlik, Anna Hać, Aleksandra Schumacher, Adriana Gucwa, Magdalena Karska, Natalia Kamińska, Jolanta Płatek, Rafał Mazuryk, Jarosław Zieliński, Jacek Kondej, Karolina Młynarz, Piotr Mucha, Piotr Skowron, Piotr Janus, Łukasz Herman-Antosiewicz, Anna Sachadyn, Paweł Czupryn, Artur Piotrowski, Arkadiusz Pikuła, Michał Rodziewicz-Motowidło, Sylwia |
author_sort | Sawicka, Justyna |
collection | PubMed |
description | Regeneration and wound healing are vital to tissue homeostasis and organism survival. One of the biggest challenges of today’s science and medicine is finding methods and factors to stimulate these processes in the human body. Effective solutions to promote regenerative responses will accelerate advances in tissue engineering, regenerative medicine, transplantology, and a number of other clinical specialties. In this study, we assessed the potential efficacy of a synthetic hexapeptide, RDKVYR, for the stimulation of tissue repair and wound healing. The hexapeptide is marketed under the name “Imunofan” (IM) as an immunostimulant. IM displayed stability in aqueous solutions, while in plasma it was rapidly bound by albumins. Structural analyses demonstrated the conformational flexibility of the peptide. Tests in human fibroblast and keratinocyte cell lines showed that IM exerted a statistically significant (p < 0.05) pro-proliferative activity (30–40% and 20–50% increase in proliferation of fibroblast and keratinocytes, respectively), revealed no cytotoxicity over a vast range of concentrations (p < 0.05), and had no allergic properties. IM was found to induce significant transcriptional responses, such as enhanced activity of genes involved in active DNA demethylation (p < 0.05) in fibroblasts and activation of genes involved in immune responses, migration, and chemotaxis in adipose-derived stem cells derived from surgery donors. Experiments in a model of ear pinna injury in mice indicated that IM moderately promoted tissue repair (8% in BALB/c and 36% in C57BL/6 in comparison to control). |
format | Online Article Text |
id | pubmed-7355430 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-73554302020-07-23 Imunofan—RDKVYR Peptide—Stimulates Skin Cell Proliferation and Promotes Tissue Repair Sawicka, Justyna Dzierżyńska, Maria Wardowska, Anna Deptuła, Milena Rogujski, Piotr Sosnowski, Paweł Filipowicz, Natalia Mieczkowska, Alina Sass, Piotr Pawlik, Anna Hać, Aleksandra Schumacher, Adriana Gucwa, Magdalena Karska, Natalia Kamińska, Jolanta Płatek, Rafał Mazuryk, Jarosław Zieliński, Jacek Kondej, Karolina Młynarz, Piotr Mucha, Piotr Skowron, Piotr Janus, Łukasz Herman-Antosiewicz, Anna Sachadyn, Paweł Czupryn, Artur Piotrowski, Arkadiusz Pikuła, Michał Rodziewicz-Motowidło, Sylwia Molecules Article Regeneration and wound healing are vital to tissue homeostasis and organism survival. One of the biggest challenges of today’s science and medicine is finding methods and factors to stimulate these processes in the human body. Effective solutions to promote regenerative responses will accelerate advances in tissue engineering, regenerative medicine, transplantology, and a number of other clinical specialties. In this study, we assessed the potential efficacy of a synthetic hexapeptide, RDKVYR, for the stimulation of tissue repair and wound healing. The hexapeptide is marketed under the name “Imunofan” (IM) as an immunostimulant. IM displayed stability in aqueous solutions, while in plasma it was rapidly bound by albumins. Structural analyses demonstrated the conformational flexibility of the peptide. Tests in human fibroblast and keratinocyte cell lines showed that IM exerted a statistically significant (p < 0.05) pro-proliferative activity (30–40% and 20–50% increase in proliferation of fibroblast and keratinocytes, respectively), revealed no cytotoxicity over a vast range of concentrations (p < 0.05), and had no allergic properties. IM was found to induce significant transcriptional responses, such as enhanced activity of genes involved in active DNA demethylation (p < 0.05) in fibroblasts and activation of genes involved in immune responses, migration, and chemotaxis in adipose-derived stem cells derived from surgery donors. Experiments in a model of ear pinna injury in mice indicated that IM moderately promoted tissue repair (8% in BALB/c and 36% in C57BL/6 in comparison to control). MDPI 2020-06-23 /pmc/articles/PMC7355430/ /pubmed/32585846 http://dx.doi.org/10.3390/molecules25122884 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Sawicka, Justyna Dzierżyńska, Maria Wardowska, Anna Deptuła, Milena Rogujski, Piotr Sosnowski, Paweł Filipowicz, Natalia Mieczkowska, Alina Sass, Piotr Pawlik, Anna Hać, Aleksandra Schumacher, Adriana Gucwa, Magdalena Karska, Natalia Kamińska, Jolanta Płatek, Rafał Mazuryk, Jarosław Zieliński, Jacek Kondej, Karolina Młynarz, Piotr Mucha, Piotr Skowron, Piotr Janus, Łukasz Herman-Antosiewicz, Anna Sachadyn, Paweł Czupryn, Artur Piotrowski, Arkadiusz Pikuła, Michał Rodziewicz-Motowidło, Sylwia Imunofan—RDKVYR Peptide—Stimulates Skin Cell Proliferation and Promotes Tissue Repair |
title | Imunofan—RDKVYR Peptide—Stimulates Skin Cell Proliferation and Promotes Tissue Repair |
title_full | Imunofan—RDKVYR Peptide—Stimulates Skin Cell Proliferation and Promotes Tissue Repair |
title_fullStr | Imunofan—RDKVYR Peptide—Stimulates Skin Cell Proliferation and Promotes Tissue Repair |
title_full_unstemmed | Imunofan—RDKVYR Peptide—Stimulates Skin Cell Proliferation and Promotes Tissue Repair |
title_short | Imunofan—RDKVYR Peptide—Stimulates Skin Cell Proliferation and Promotes Tissue Repair |
title_sort | imunofan—rdkvyr peptide—stimulates skin cell proliferation and promotes tissue repair |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7355430/ https://www.ncbi.nlm.nih.gov/pubmed/32585846 http://dx.doi.org/10.3390/molecules25122884 |
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