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Liposome-Embedding Silicon Microparticle for Oxaliplatin Delivery in Tumor Chemotherapy

Mesoporous silicon microparticles (MSMPs) can incorporate drug-carrying nanoparticles (NPs) into their pores. An NP-loaded MSMP is a multistage vector (MSV) that forms a Matryoshka-like structure that protects the therapeutic cargo from degradation and prevents its dilution in the circulation during...

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Autores principales: Cevenini, Armando, Celia, Christian, Orrù, Stefania, Sarnataro, Daniela, Raia, Maddalena, Mollo, Valentina, Locatelli, Marcello, Imperlini, Esther, Peluso, Nicoletta, Peltrini, Rosa, De Rosa, Enrica, Parodi, Alessandro, Del Vecchio, Luigi, Di Marzio, Luisa, Fresta, Massimo, Netti, Paolo Antonio, Shen, Haifa, Liu, Xuewu, Tasciotti, Ennio, Salvatore, Francesco
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7355455/
https://www.ncbi.nlm.nih.gov/pubmed/32560359
http://dx.doi.org/10.3390/pharmaceutics12060559
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author Cevenini, Armando
Celia, Christian
Orrù, Stefania
Sarnataro, Daniela
Raia, Maddalena
Mollo, Valentina
Locatelli, Marcello
Imperlini, Esther
Peluso, Nicoletta
Peltrini, Rosa
De Rosa, Enrica
Parodi, Alessandro
Del Vecchio, Luigi
Di Marzio, Luisa
Fresta, Massimo
Netti, Paolo Antonio
Shen, Haifa
Liu, Xuewu
Tasciotti, Ennio
Salvatore, Francesco
author_facet Cevenini, Armando
Celia, Christian
Orrù, Stefania
Sarnataro, Daniela
Raia, Maddalena
Mollo, Valentina
Locatelli, Marcello
Imperlini, Esther
Peluso, Nicoletta
Peltrini, Rosa
De Rosa, Enrica
Parodi, Alessandro
Del Vecchio, Luigi
Di Marzio, Luisa
Fresta, Massimo
Netti, Paolo Antonio
Shen, Haifa
Liu, Xuewu
Tasciotti, Ennio
Salvatore, Francesco
author_sort Cevenini, Armando
collection PubMed
description Mesoporous silicon microparticles (MSMPs) can incorporate drug-carrying nanoparticles (NPs) into their pores. An NP-loaded MSMP is a multistage vector (MSV) that forms a Matryoshka-like structure that protects the therapeutic cargo from degradation and prevents its dilution in the circulation during delivery to tumor cells. We developed an MSV constituted by 1 µm discoidal MSMPs embedded with PEGylated liposomes containing oxaliplatin (oxa) which is a therapeutic agent for colorectal cancer (CRC). To obtain extra-small liposomes able to fit the 60 nm pores of MSMP, we tested several liposomal formulations, and identified two optimal compositions, with a prevalence of the rigid lipid 1,2-distearoyl-sn-glycero-3-phosphocholine and of 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[methoxy(polyethylene glycol)-2000]. To improve the MSV assembly, we optimized the liposome-loading inside the MSMP and achieved a five-fold increase of the payload using an innovative lyophilization approach. This procedure also increased the load and limited dimensional changes of the liposomes released from the MSV in vitro. Lastly, we found that the cytotoxic efficacy of oxa-loaded liposomes and-oxa-liposome-MSV in CRC cell culture was similar to that of free oxa. This study increases knowledge about extra-small liposomes and their loading into porous materials and provides useful hints about alternative strategies for designing drug-encapsulating NPs.
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spelling pubmed-73554552020-07-23 Liposome-Embedding Silicon Microparticle for Oxaliplatin Delivery in Tumor Chemotherapy Cevenini, Armando Celia, Christian Orrù, Stefania Sarnataro, Daniela Raia, Maddalena Mollo, Valentina Locatelli, Marcello Imperlini, Esther Peluso, Nicoletta Peltrini, Rosa De Rosa, Enrica Parodi, Alessandro Del Vecchio, Luigi Di Marzio, Luisa Fresta, Massimo Netti, Paolo Antonio Shen, Haifa Liu, Xuewu Tasciotti, Ennio Salvatore, Francesco Pharmaceutics Article Mesoporous silicon microparticles (MSMPs) can incorporate drug-carrying nanoparticles (NPs) into their pores. An NP-loaded MSMP is a multistage vector (MSV) that forms a Matryoshka-like structure that protects the therapeutic cargo from degradation and prevents its dilution in the circulation during delivery to tumor cells. We developed an MSV constituted by 1 µm discoidal MSMPs embedded with PEGylated liposomes containing oxaliplatin (oxa) which is a therapeutic agent for colorectal cancer (CRC). To obtain extra-small liposomes able to fit the 60 nm pores of MSMP, we tested several liposomal formulations, and identified two optimal compositions, with a prevalence of the rigid lipid 1,2-distearoyl-sn-glycero-3-phosphocholine and of 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[methoxy(polyethylene glycol)-2000]. To improve the MSV assembly, we optimized the liposome-loading inside the MSMP and achieved a five-fold increase of the payload using an innovative lyophilization approach. This procedure also increased the load and limited dimensional changes of the liposomes released from the MSV in vitro. Lastly, we found that the cytotoxic efficacy of oxa-loaded liposomes and-oxa-liposome-MSV in CRC cell culture was similar to that of free oxa. This study increases knowledge about extra-small liposomes and their loading into porous materials and provides useful hints about alternative strategies for designing drug-encapsulating NPs. MDPI 2020-06-17 /pmc/articles/PMC7355455/ /pubmed/32560359 http://dx.doi.org/10.3390/pharmaceutics12060559 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Cevenini, Armando
Celia, Christian
Orrù, Stefania
Sarnataro, Daniela
Raia, Maddalena
Mollo, Valentina
Locatelli, Marcello
Imperlini, Esther
Peluso, Nicoletta
Peltrini, Rosa
De Rosa, Enrica
Parodi, Alessandro
Del Vecchio, Luigi
Di Marzio, Luisa
Fresta, Massimo
Netti, Paolo Antonio
Shen, Haifa
Liu, Xuewu
Tasciotti, Ennio
Salvatore, Francesco
Liposome-Embedding Silicon Microparticle for Oxaliplatin Delivery in Tumor Chemotherapy
title Liposome-Embedding Silicon Microparticle for Oxaliplatin Delivery in Tumor Chemotherapy
title_full Liposome-Embedding Silicon Microparticle for Oxaliplatin Delivery in Tumor Chemotherapy
title_fullStr Liposome-Embedding Silicon Microparticle for Oxaliplatin Delivery in Tumor Chemotherapy
title_full_unstemmed Liposome-Embedding Silicon Microparticle for Oxaliplatin Delivery in Tumor Chemotherapy
title_short Liposome-Embedding Silicon Microparticle for Oxaliplatin Delivery in Tumor Chemotherapy
title_sort liposome-embedding silicon microparticle for oxaliplatin delivery in tumor chemotherapy
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7355455/
https://www.ncbi.nlm.nih.gov/pubmed/32560359
http://dx.doi.org/10.3390/pharmaceutics12060559
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