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Recovery of Vaginal Microbiota after Standard Treatment for Bacterial Vaginosis Infection: An Observational Study

Vaginal microbiota dysbiosis and bacterial vaginosis (BV) affect negatively women’s health. Understanding vaginal microbiota fluctuations in BV during and after antibiotic treatment would facilitate accurate decision-making on the treatment regimen, avoid unnecessary antibiotic use, and potentially...

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Autores principales: Lehtoranta, Liisa, Hibberd, Ashley A., Reimari, Jenni, Junnila, Jouni, Yeung, Nicolas, Maukonen, Johanna, Crawford, Gordon, Ouwehand, Arthur C.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7355544/
https://www.ncbi.nlm.nih.gov/pubmed/32527048
http://dx.doi.org/10.3390/microorganisms8060875
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author Lehtoranta, Liisa
Hibberd, Ashley A.
Reimari, Jenni
Junnila, Jouni
Yeung, Nicolas
Maukonen, Johanna
Crawford, Gordon
Ouwehand, Arthur C.
author_facet Lehtoranta, Liisa
Hibberd, Ashley A.
Reimari, Jenni
Junnila, Jouni
Yeung, Nicolas
Maukonen, Johanna
Crawford, Gordon
Ouwehand, Arthur C.
author_sort Lehtoranta, Liisa
collection PubMed
description Vaginal microbiota dysbiosis and bacterial vaginosis (BV) affect negatively women’s health. Understanding vaginal microbiota fluctuations in BV during and after antibiotic treatment would facilitate accurate decision-making on the treatment regimen, avoid unnecessary antibiotic use, and potentially mitigate recurrence. We investigated vaginal microbiota composition of 30 women with BV before and after 5-day metronidazole treatment and compared the results with 30 healthy women. Vaginal microbiota was assessed by Nugent score and analyzed by 16S rRNA gene sequencing in swabs on baseline Day 1, and on Day 8 and 15, after completion of antibiotic treatment by women with BV. Prior to antibiotic treatment (Day 1), BV-positive women were dominated by Lactobacillus iners (25.8%), Prevotella timonensis/bivia (18.0%), and Gardnerella vaginalis (14.6%), whereas healthy women were dominated by L. iners (37.5%) and Lactobacillus crispatus/acidophilus (19.2%). On Day 8, L. iners abundance increased in BV-treated women being significantly higher compared with healthy women (67.8% vs. 37.5%, p = 0.049). On Day 15, the relative abundance of all microbial taxa was similar between the groups. Vaginal microbiota of women with BV shifted to resemble that of healthy controls after metronidazole. Sequencing analysis provides more in-depth understanding of changes in vaginal microbiota. The role of L. iners in vaginal health and dysbiosis requires further investigations.
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spelling pubmed-73555442020-07-23 Recovery of Vaginal Microbiota after Standard Treatment for Bacterial Vaginosis Infection: An Observational Study Lehtoranta, Liisa Hibberd, Ashley A. Reimari, Jenni Junnila, Jouni Yeung, Nicolas Maukonen, Johanna Crawford, Gordon Ouwehand, Arthur C. Microorganisms Article Vaginal microbiota dysbiosis and bacterial vaginosis (BV) affect negatively women’s health. Understanding vaginal microbiota fluctuations in BV during and after antibiotic treatment would facilitate accurate decision-making on the treatment regimen, avoid unnecessary antibiotic use, and potentially mitigate recurrence. We investigated vaginal microbiota composition of 30 women with BV before and after 5-day metronidazole treatment and compared the results with 30 healthy women. Vaginal microbiota was assessed by Nugent score and analyzed by 16S rRNA gene sequencing in swabs on baseline Day 1, and on Day 8 and 15, after completion of antibiotic treatment by women with BV. Prior to antibiotic treatment (Day 1), BV-positive women were dominated by Lactobacillus iners (25.8%), Prevotella timonensis/bivia (18.0%), and Gardnerella vaginalis (14.6%), whereas healthy women were dominated by L. iners (37.5%) and Lactobacillus crispatus/acidophilus (19.2%). On Day 8, L. iners abundance increased in BV-treated women being significantly higher compared with healthy women (67.8% vs. 37.5%, p = 0.049). On Day 15, the relative abundance of all microbial taxa was similar between the groups. Vaginal microbiota of women with BV shifted to resemble that of healthy controls after metronidazole. Sequencing analysis provides more in-depth understanding of changes in vaginal microbiota. The role of L. iners in vaginal health and dysbiosis requires further investigations. MDPI 2020-06-09 /pmc/articles/PMC7355544/ /pubmed/32527048 http://dx.doi.org/10.3390/microorganisms8060875 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Lehtoranta, Liisa
Hibberd, Ashley A.
Reimari, Jenni
Junnila, Jouni
Yeung, Nicolas
Maukonen, Johanna
Crawford, Gordon
Ouwehand, Arthur C.
Recovery of Vaginal Microbiota after Standard Treatment for Bacterial Vaginosis Infection: An Observational Study
title Recovery of Vaginal Microbiota after Standard Treatment for Bacterial Vaginosis Infection: An Observational Study
title_full Recovery of Vaginal Microbiota after Standard Treatment for Bacterial Vaginosis Infection: An Observational Study
title_fullStr Recovery of Vaginal Microbiota after Standard Treatment for Bacterial Vaginosis Infection: An Observational Study
title_full_unstemmed Recovery of Vaginal Microbiota after Standard Treatment for Bacterial Vaginosis Infection: An Observational Study
title_short Recovery of Vaginal Microbiota after Standard Treatment for Bacterial Vaginosis Infection: An Observational Study
title_sort recovery of vaginal microbiota after standard treatment for bacterial vaginosis infection: an observational study
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7355544/
https://www.ncbi.nlm.nih.gov/pubmed/32527048
http://dx.doi.org/10.3390/microorganisms8060875
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