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Impaired Fibrinolysis in Patients with Isolated Aortic Stenosis is Associated with Enhanced Oxidative Stress

Aortic stenosis (AS) has been associated with impaired fibrinolysis and increased oxidative stress. This study aimed to investigate whether oxidative stress could alter fibrin clot properties in AS. We studied 173 non-diabetic patients, aged 51–79 years, with isolated AS. We measured plasma protein...

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Autores principales: Siudut, Jakub, Natorska, Joanna, Wypasek, Ewa, Wiewiórka, Łukasz, Ostrowska-Kaim, Elżbieta, Wiśniowska-Śmiałek, Sylwia, Plens, Krzysztof, Legutko, Jacek, Undas, Anetta
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7355626/
https://www.ncbi.nlm.nih.gov/pubmed/32630544
http://dx.doi.org/10.3390/jcm9062002
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author Siudut, Jakub
Natorska, Joanna
Wypasek, Ewa
Wiewiórka, Łukasz
Ostrowska-Kaim, Elżbieta
Wiśniowska-Śmiałek, Sylwia
Plens, Krzysztof
Legutko, Jacek
Undas, Anetta
author_facet Siudut, Jakub
Natorska, Joanna
Wypasek, Ewa
Wiewiórka, Łukasz
Ostrowska-Kaim, Elżbieta
Wiśniowska-Śmiałek, Sylwia
Plens, Krzysztof
Legutko, Jacek
Undas, Anetta
author_sort Siudut, Jakub
collection PubMed
description Aortic stenosis (AS) has been associated with impaired fibrinolysis and increased oxidative stress. This study aimed to investigate whether oxidative stress could alter fibrin clot properties in AS. We studied 173 non-diabetic patients, aged 51–79 years, with isolated AS. We measured plasma protein carbonylation (PC) and thiobarbituric acid reactive substances (TBARS), along with plasma clot permeability (K(s)), thrombin generation, and fibrinolytic efficiency, which were evaluated by two assays: clot lysis time (CLT) and lysis time (Lys50). Coagulation factors and fibrinolytic proteins were also determined. Plasma PC showed an association with AS severity, reflected by the aortic valve area and the mean and maximum aortic gradients. Plasma PC was positively correlated with CLT, Lys50, plasminogen activator inhibitor-1 (PAI-1), and tissue factor (TF) antigens. TBARS were positively correlated with maximum aortic gradient, Lys50, and TF antigen. Regression analysis showed that PC predicted prolonged CLT (>104 min; odds ratio (OR) 6.41, 95% confidence interval (CI) 2.58–17.83, p < 0.001) and Lys50 (>565 s; OR 5.83, 95% CI 2.23–15.21, p < 0.001). Multivariate regression analysis showed that mean aortic gradient, PC, α2-antiplasmin, PAI-1, and triglycerides were predictors of prolonged CLT, while PC, α2-antiplasmin, and fibrinogen were predictors of Lys50. Our findings suggest that elevated oxidative stress contributes to impaired fibrinolysis in AS and is associated with AS severity.
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spelling pubmed-73556262020-07-23 Impaired Fibrinolysis in Patients with Isolated Aortic Stenosis is Associated with Enhanced Oxidative Stress Siudut, Jakub Natorska, Joanna Wypasek, Ewa Wiewiórka, Łukasz Ostrowska-Kaim, Elżbieta Wiśniowska-Śmiałek, Sylwia Plens, Krzysztof Legutko, Jacek Undas, Anetta J Clin Med Article Aortic stenosis (AS) has been associated with impaired fibrinolysis and increased oxidative stress. This study aimed to investigate whether oxidative stress could alter fibrin clot properties in AS. We studied 173 non-diabetic patients, aged 51–79 years, with isolated AS. We measured plasma protein carbonylation (PC) and thiobarbituric acid reactive substances (TBARS), along with plasma clot permeability (K(s)), thrombin generation, and fibrinolytic efficiency, which were evaluated by two assays: clot lysis time (CLT) and lysis time (Lys50). Coagulation factors and fibrinolytic proteins were also determined. Plasma PC showed an association with AS severity, reflected by the aortic valve area and the mean and maximum aortic gradients. Plasma PC was positively correlated with CLT, Lys50, plasminogen activator inhibitor-1 (PAI-1), and tissue factor (TF) antigens. TBARS were positively correlated with maximum aortic gradient, Lys50, and TF antigen. Regression analysis showed that PC predicted prolonged CLT (>104 min; odds ratio (OR) 6.41, 95% confidence interval (CI) 2.58–17.83, p < 0.001) and Lys50 (>565 s; OR 5.83, 95% CI 2.23–15.21, p < 0.001). Multivariate regression analysis showed that mean aortic gradient, PC, α2-antiplasmin, PAI-1, and triglycerides were predictors of prolonged CLT, while PC, α2-antiplasmin, and fibrinogen were predictors of Lys50. Our findings suggest that elevated oxidative stress contributes to impaired fibrinolysis in AS and is associated with AS severity. MDPI 2020-06-25 /pmc/articles/PMC7355626/ /pubmed/32630544 http://dx.doi.org/10.3390/jcm9062002 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Siudut, Jakub
Natorska, Joanna
Wypasek, Ewa
Wiewiórka, Łukasz
Ostrowska-Kaim, Elżbieta
Wiśniowska-Śmiałek, Sylwia
Plens, Krzysztof
Legutko, Jacek
Undas, Anetta
Impaired Fibrinolysis in Patients with Isolated Aortic Stenosis is Associated with Enhanced Oxidative Stress
title Impaired Fibrinolysis in Patients with Isolated Aortic Stenosis is Associated with Enhanced Oxidative Stress
title_full Impaired Fibrinolysis in Patients with Isolated Aortic Stenosis is Associated with Enhanced Oxidative Stress
title_fullStr Impaired Fibrinolysis in Patients with Isolated Aortic Stenosis is Associated with Enhanced Oxidative Stress
title_full_unstemmed Impaired Fibrinolysis in Patients with Isolated Aortic Stenosis is Associated with Enhanced Oxidative Stress
title_short Impaired Fibrinolysis in Patients with Isolated Aortic Stenosis is Associated with Enhanced Oxidative Stress
title_sort impaired fibrinolysis in patients with isolated aortic stenosis is associated with enhanced oxidative stress
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7355626/
https://www.ncbi.nlm.nih.gov/pubmed/32630544
http://dx.doi.org/10.3390/jcm9062002
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