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Optimization of QuEChERS Method for Simultaneous Determination of Neonicotinoid Residues in Pollinator Forage

Consistent with the large-scale use of pesticide seed treatments in U.S. field crop production, there has been an increased use of neonicotinoid-treated corn and soybean seed over the past decade. Neonicotinoids can move downwind to adjacent off-field pollinator habitats in dust from planting and/or...

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Autores principales: Hall, Maura J., Dang, Viet, Bradbury, Steven P., Coats, Joel R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7355641/
https://www.ncbi.nlm.nih.gov/pubmed/32545582
http://dx.doi.org/10.3390/molecules25122732
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author Hall, Maura J.
Dang, Viet
Bradbury, Steven P.
Coats, Joel R.
author_facet Hall, Maura J.
Dang, Viet
Bradbury, Steven P.
Coats, Joel R.
author_sort Hall, Maura J.
collection PubMed
description Consistent with the large-scale use of pesticide seed treatments in U.S. field crop production, there has been an increased use of neonicotinoid-treated corn and soybean seed over the past decade. Neonicotinoids can move downwind to adjacent off-field pollinator habitats in dust from planting and/or move downslope to habitats in surface water. The extent of potential neonicotinoid exposure to pollinators from neonicotinoid movement into these adjacent pollinator habitats is unclear. Pollen and leaf tissue extractions were completed using a quick, easy, cheap, effective, rugged, and safe (QuEChERS) extraction procedure. Samples were subjected to a clean-up step using dispersive solid-phase extraction (dSPE) techniques prior to analysis. The compounds in the extracts were separated on a reversed-phase column with gradient elution and confirmed with tandem mass spectrometry. The extraction method showed acceptable recoveries of analytes ranging from 78.4 to 93.6% and 89.4 to 101% for leaf tissue and pollen, respectively. The method’s detection limits ranged from 0.04 to 0.3 ng/g in milkweed leaf tissue and 0.04 to 1.0 ng/g in pollen. The method is currently being employed in ongoing studies surveying pollen from a diversity of forbs and milkweed leaves obtained from habitat patches established within fields with a history of using neonicotinoid-treated seeds.
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spelling pubmed-73556412020-07-23 Optimization of QuEChERS Method for Simultaneous Determination of Neonicotinoid Residues in Pollinator Forage Hall, Maura J. Dang, Viet Bradbury, Steven P. Coats, Joel R. Molecules Article Consistent with the large-scale use of pesticide seed treatments in U.S. field crop production, there has been an increased use of neonicotinoid-treated corn and soybean seed over the past decade. Neonicotinoids can move downwind to adjacent off-field pollinator habitats in dust from planting and/or move downslope to habitats in surface water. The extent of potential neonicotinoid exposure to pollinators from neonicotinoid movement into these adjacent pollinator habitats is unclear. Pollen and leaf tissue extractions were completed using a quick, easy, cheap, effective, rugged, and safe (QuEChERS) extraction procedure. Samples were subjected to a clean-up step using dispersive solid-phase extraction (dSPE) techniques prior to analysis. The compounds in the extracts were separated on a reversed-phase column with gradient elution and confirmed with tandem mass spectrometry. The extraction method showed acceptable recoveries of analytes ranging from 78.4 to 93.6% and 89.4 to 101% for leaf tissue and pollen, respectively. The method’s detection limits ranged from 0.04 to 0.3 ng/g in milkweed leaf tissue and 0.04 to 1.0 ng/g in pollen. The method is currently being employed in ongoing studies surveying pollen from a diversity of forbs and milkweed leaves obtained from habitat patches established within fields with a history of using neonicotinoid-treated seeds. MDPI 2020-06-12 /pmc/articles/PMC7355641/ /pubmed/32545582 http://dx.doi.org/10.3390/molecules25122732 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Hall, Maura J.
Dang, Viet
Bradbury, Steven P.
Coats, Joel R.
Optimization of QuEChERS Method for Simultaneous Determination of Neonicotinoid Residues in Pollinator Forage
title Optimization of QuEChERS Method for Simultaneous Determination of Neonicotinoid Residues in Pollinator Forage
title_full Optimization of QuEChERS Method for Simultaneous Determination of Neonicotinoid Residues in Pollinator Forage
title_fullStr Optimization of QuEChERS Method for Simultaneous Determination of Neonicotinoid Residues in Pollinator Forage
title_full_unstemmed Optimization of QuEChERS Method for Simultaneous Determination of Neonicotinoid Residues in Pollinator Forage
title_short Optimization of QuEChERS Method for Simultaneous Determination of Neonicotinoid Residues in Pollinator Forage
title_sort optimization of quechers method for simultaneous determination of neonicotinoid residues in pollinator forage
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7355641/
https://www.ncbi.nlm.nih.gov/pubmed/32545582
http://dx.doi.org/10.3390/molecules25122732
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