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Development of Antibody–Oligonucleotide Complexes for Targeting Exosomal MicroRNA

MicroRNAs in exosomes (exosomal miRNAs) are considered as significant targets for cancer therapy. Anti-miR oligonucleotides are often used for the functional inhibition of miRNAs; however, there are no studies regarding the regulation of exosomal miRNA functions. In this study, we attempted to devel...

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Detalles Bibliográficos
Autores principales: Yamayoshi, Asako, Oyama, Shota, Kishimoto, Yusuke, Konishi, Ryo, Yamamoto, Tsuyoshi, Kobori, Akio, Harada, Hiroshi, Ashihara, Eishi, Sugiyama, Hiroshi, Murakami, Akira
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7355672/
https://www.ncbi.nlm.nih.gov/pubmed/32545479
http://dx.doi.org/10.3390/pharmaceutics12060545
Descripción
Sumario:MicroRNAs in exosomes (exosomal miRNAs) are considered as significant targets for cancer therapy. Anti-miR oligonucleotides are often used for the functional inhibition of miRNAs; however, there are no studies regarding the regulation of exosomal miRNA functions. In this study, we attempted to develop a novel drug delivery system using anti-exosome antibody–anti-miR oligonucleotide complexes (ExomiR-Tracker) to hijack exosomes to carry anti-miR oligonucleotides inside exosome-recipient cells. We found that ExomiR-Tracker bound to the exosomes, and then the complexes were introduced into the recipient cells. We also found that anti-miR oligonucleotides introduced into the recipient cells can exhibit inhibitory effects on exosomal miRNA functions in vitro and in vivo. We believe that our strategy would be a promising one for targeting exosomal miRNAs.