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Development of Antibody–Oligonucleotide Complexes for Targeting Exosomal MicroRNA

MicroRNAs in exosomes (exosomal miRNAs) are considered as significant targets for cancer therapy. Anti-miR oligonucleotides are often used for the functional inhibition of miRNAs; however, there are no studies regarding the regulation of exosomal miRNA functions. In this study, we attempted to devel...

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Autores principales: Yamayoshi, Asako, Oyama, Shota, Kishimoto, Yusuke, Konishi, Ryo, Yamamoto, Tsuyoshi, Kobori, Akio, Harada, Hiroshi, Ashihara, Eishi, Sugiyama, Hiroshi, Murakami, Akira
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7355672/
https://www.ncbi.nlm.nih.gov/pubmed/32545479
http://dx.doi.org/10.3390/pharmaceutics12060545
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author Yamayoshi, Asako
Oyama, Shota
Kishimoto, Yusuke
Konishi, Ryo
Yamamoto, Tsuyoshi
Kobori, Akio
Harada, Hiroshi
Ashihara, Eishi
Sugiyama, Hiroshi
Murakami, Akira
author_facet Yamayoshi, Asako
Oyama, Shota
Kishimoto, Yusuke
Konishi, Ryo
Yamamoto, Tsuyoshi
Kobori, Akio
Harada, Hiroshi
Ashihara, Eishi
Sugiyama, Hiroshi
Murakami, Akira
author_sort Yamayoshi, Asako
collection PubMed
description MicroRNAs in exosomes (exosomal miRNAs) are considered as significant targets for cancer therapy. Anti-miR oligonucleotides are often used for the functional inhibition of miRNAs; however, there are no studies regarding the regulation of exosomal miRNA functions. In this study, we attempted to develop a novel drug delivery system using anti-exosome antibody–anti-miR oligonucleotide complexes (ExomiR-Tracker) to hijack exosomes to carry anti-miR oligonucleotides inside exosome-recipient cells. We found that ExomiR-Tracker bound to the exosomes, and then the complexes were introduced into the recipient cells. We also found that anti-miR oligonucleotides introduced into the recipient cells can exhibit inhibitory effects on exosomal miRNA functions in vitro and in vivo. We believe that our strategy would be a promising one for targeting exosomal miRNAs.
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spelling pubmed-73556722020-07-23 Development of Antibody–Oligonucleotide Complexes for Targeting Exosomal MicroRNA Yamayoshi, Asako Oyama, Shota Kishimoto, Yusuke Konishi, Ryo Yamamoto, Tsuyoshi Kobori, Akio Harada, Hiroshi Ashihara, Eishi Sugiyama, Hiroshi Murakami, Akira Pharmaceutics Article MicroRNAs in exosomes (exosomal miRNAs) are considered as significant targets for cancer therapy. Anti-miR oligonucleotides are often used for the functional inhibition of miRNAs; however, there are no studies regarding the regulation of exosomal miRNA functions. In this study, we attempted to develop a novel drug delivery system using anti-exosome antibody–anti-miR oligonucleotide complexes (ExomiR-Tracker) to hijack exosomes to carry anti-miR oligonucleotides inside exosome-recipient cells. We found that ExomiR-Tracker bound to the exosomes, and then the complexes were introduced into the recipient cells. We also found that anti-miR oligonucleotides introduced into the recipient cells can exhibit inhibitory effects on exosomal miRNA functions in vitro and in vivo. We believe that our strategy would be a promising one for targeting exosomal miRNAs. MDPI 2020-06-12 /pmc/articles/PMC7355672/ /pubmed/32545479 http://dx.doi.org/10.3390/pharmaceutics12060545 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Yamayoshi, Asako
Oyama, Shota
Kishimoto, Yusuke
Konishi, Ryo
Yamamoto, Tsuyoshi
Kobori, Akio
Harada, Hiroshi
Ashihara, Eishi
Sugiyama, Hiroshi
Murakami, Akira
Development of Antibody–Oligonucleotide Complexes for Targeting Exosomal MicroRNA
title Development of Antibody–Oligonucleotide Complexes for Targeting Exosomal MicroRNA
title_full Development of Antibody–Oligonucleotide Complexes for Targeting Exosomal MicroRNA
title_fullStr Development of Antibody–Oligonucleotide Complexes for Targeting Exosomal MicroRNA
title_full_unstemmed Development of Antibody–Oligonucleotide Complexes for Targeting Exosomal MicroRNA
title_short Development of Antibody–Oligonucleotide Complexes for Targeting Exosomal MicroRNA
title_sort development of antibody–oligonucleotide complexes for targeting exosomal microrna
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7355672/
https://www.ncbi.nlm.nih.gov/pubmed/32545479
http://dx.doi.org/10.3390/pharmaceutics12060545
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