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Development of Antibody–Oligonucleotide Complexes for Targeting Exosomal MicroRNA
MicroRNAs in exosomes (exosomal miRNAs) are considered as significant targets for cancer therapy. Anti-miR oligonucleotides are often used for the functional inhibition of miRNAs; however, there are no studies regarding the regulation of exosomal miRNA functions. In this study, we attempted to devel...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7355672/ https://www.ncbi.nlm.nih.gov/pubmed/32545479 http://dx.doi.org/10.3390/pharmaceutics12060545 |
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author | Yamayoshi, Asako Oyama, Shota Kishimoto, Yusuke Konishi, Ryo Yamamoto, Tsuyoshi Kobori, Akio Harada, Hiroshi Ashihara, Eishi Sugiyama, Hiroshi Murakami, Akira |
author_facet | Yamayoshi, Asako Oyama, Shota Kishimoto, Yusuke Konishi, Ryo Yamamoto, Tsuyoshi Kobori, Akio Harada, Hiroshi Ashihara, Eishi Sugiyama, Hiroshi Murakami, Akira |
author_sort | Yamayoshi, Asako |
collection | PubMed |
description | MicroRNAs in exosomes (exosomal miRNAs) are considered as significant targets for cancer therapy. Anti-miR oligonucleotides are often used for the functional inhibition of miRNAs; however, there are no studies regarding the regulation of exosomal miRNA functions. In this study, we attempted to develop a novel drug delivery system using anti-exosome antibody–anti-miR oligonucleotide complexes (ExomiR-Tracker) to hijack exosomes to carry anti-miR oligonucleotides inside exosome-recipient cells. We found that ExomiR-Tracker bound to the exosomes, and then the complexes were introduced into the recipient cells. We also found that anti-miR oligonucleotides introduced into the recipient cells can exhibit inhibitory effects on exosomal miRNA functions in vitro and in vivo. We believe that our strategy would be a promising one for targeting exosomal miRNAs. |
format | Online Article Text |
id | pubmed-7355672 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-73556722020-07-23 Development of Antibody–Oligonucleotide Complexes for Targeting Exosomal MicroRNA Yamayoshi, Asako Oyama, Shota Kishimoto, Yusuke Konishi, Ryo Yamamoto, Tsuyoshi Kobori, Akio Harada, Hiroshi Ashihara, Eishi Sugiyama, Hiroshi Murakami, Akira Pharmaceutics Article MicroRNAs in exosomes (exosomal miRNAs) are considered as significant targets for cancer therapy. Anti-miR oligonucleotides are often used for the functional inhibition of miRNAs; however, there are no studies regarding the regulation of exosomal miRNA functions. In this study, we attempted to develop a novel drug delivery system using anti-exosome antibody–anti-miR oligonucleotide complexes (ExomiR-Tracker) to hijack exosomes to carry anti-miR oligonucleotides inside exosome-recipient cells. We found that ExomiR-Tracker bound to the exosomes, and then the complexes were introduced into the recipient cells. We also found that anti-miR oligonucleotides introduced into the recipient cells can exhibit inhibitory effects on exosomal miRNA functions in vitro and in vivo. We believe that our strategy would be a promising one for targeting exosomal miRNAs. MDPI 2020-06-12 /pmc/articles/PMC7355672/ /pubmed/32545479 http://dx.doi.org/10.3390/pharmaceutics12060545 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Yamayoshi, Asako Oyama, Shota Kishimoto, Yusuke Konishi, Ryo Yamamoto, Tsuyoshi Kobori, Akio Harada, Hiroshi Ashihara, Eishi Sugiyama, Hiroshi Murakami, Akira Development of Antibody–Oligonucleotide Complexes for Targeting Exosomal MicroRNA |
title | Development of Antibody–Oligonucleotide Complexes for Targeting Exosomal MicroRNA |
title_full | Development of Antibody–Oligonucleotide Complexes for Targeting Exosomal MicroRNA |
title_fullStr | Development of Antibody–Oligonucleotide Complexes for Targeting Exosomal MicroRNA |
title_full_unstemmed | Development of Antibody–Oligonucleotide Complexes for Targeting Exosomal MicroRNA |
title_short | Development of Antibody–Oligonucleotide Complexes for Targeting Exosomal MicroRNA |
title_sort | development of antibody–oligonucleotide complexes for targeting exosomal microrna |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7355672/ https://www.ncbi.nlm.nih.gov/pubmed/32545479 http://dx.doi.org/10.3390/pharmaceutics12060545 |
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