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Easy-to-Use InDel Markers for Genetic Mapping between Col-0 and Ler-0 Accessions of Arabidopsis thaliana

Map-based gene cloning has played a key role in many genetic studies using the model plant, Arabidopsis thaliana. In the post- next generation sequencing era, identification of point mutations and their corresponding genes is increasingly becoming a powerful and important approach to define plant ge...

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Autores principales: Tanaka, Takahiro, Nishii, Yuichi, Matsuo, Hirotoshi, Takahashi, Taku
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7355782/
https://www.ncbi.nlm.nih.gov/pubmed/32580428
http://dx.doi.org/10.3390/plants9060779
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author Tanaka, Takahiro
Nishii, Yuichi
Matsuo, Hirotoshi
Takahashi, Taku
author_facet Tanaka, Takahiro
Nishii, Yuichi
Matsuo, Hirotoshi
Takahashi, Taku
author_sort Tanaka, Takahiro
collection PubMed
description Map-based gene cloning has played a key role in many genetic studies using the model plant, Arabidopsis thaliana. In the post- next generation sequencing era, identification of point mutations and their corresponding genes is increasingly becoming a powerful and important approach to define plant gene function. To perform initial mapping experiments efficiently on Arabidopsis mutants, enrichment of easy-to-use and reliable polymorphic DNA markers would be desirable. We present here a list of InDel polymorphic markers between Col-0 and Ler-0 accessions that can be detected in standard agarose gel electrophoresis.
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spelling pubmed-73557822020-07-23 Easy-to-Use InDel Markers for Genetic Mapping between Col-0 and Ler-0 Accessions of Arabidopsis thaliana Tanaka, Takahiro Nishii, Yuichi Matsuo, Hirotoshi Takahashi, Taku Plants (Basel) Technical Note Map-based gene cloning has played a key role in many genetic studies using the model plant, Arabidopsis thaliana. In the post- next generation sequencing era, identification of point mutations and their corresponding genes is increasingly becoming a powerful and important approach to define plant gene function. To perform initial mapping experiments efficiently on Arabidopsis mutants, enrichment of easy-to-use and reliable polymorphic DNA markers would be desirable. We present here a list of InDel polymorphic markers between Col-0 and Ler-0 accessions that can be detected in standard agarose gel electrophoresis. MDPI 2020-06-22 /pmc/articles/PMC7355782/ /pubmed/32580428 http://dx.doi.org/10.3390/plants9060779 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Technical Note
Tanaka, Takahiro
Nishii, Yuichi
Matsuo, Hirotoshi
Takahashi, Taku
Easy-to-Use InDel Markers for Genetic Mapping between Col-0 and Ler-0 Accessions of Arabidopsis thaliana
title Easy-to-Use InDel Markers for Genetic Mapping between Col-0 and Ler-0 Accessions of Arabidopsis thaliana
title_full Easy-to-Use InDel Markers for Genetic Mapping between Col-0 and Ler-0 Accessions of Arabidopsis thaliana
title_fullStr Easy-to-Use InDel Markers for Genetic Mapping between Col-0 and Ler-0 Accessions of Arabidopsis thaliana
title_full_unstemmed Easy-to-Use InDel Markers for Genetic Mapping between Col-0 and Ler-0 Accessions of Arabidopsis thaliana
title_short Easy-to-Use InDel Markers for Genetic Mapping between Col-0 and Ler-0 Accessions of Arabidopsis thaliana
title_sort easy-to-use indel markers for genetic mapping between col-0 and ler-0 accessions of arabidopsis thaliana
topic Technical Note
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7355782/
https://www.ncbi.nlm.nih.gov/pubmed/32580428
http://dx.doi.org/10.3390/plants9060779
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