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Easy-to-Use InDel Markers for Genetic Mapping between Col-0 and Ler-0 Accessions of Arabidopsis thaliana
Map-based gene cloning has played a key role in many genetic studies using the model plant, Arabidopsis thaliana. In the post- next generation sequencing era, identification of point mutations and their corresponding genes is increasingly becoming a powerful and important approach to define plant ge...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7355782/ https://www.ncbi.nlm.nih.gov/pubmed/32580428 http://dx.doi.org/10.3390/plants9060779 |
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author | Tanaka, Takahiro Nishii, Yuichi Matsuo, Hirotoshi Takahashi, Taku |
author_facet | Tanaka, Takahiro Nishii, Yuichi Matsuo, Hirotoshi Takahashi, Taku |
author_sort | Tanaka, Takahiro |
collection | PubMed |
description | Map-based gene cloning has played a key role in many genetic studies using the model plant, Arabidopsis thaliana. In the post- next generation sequencing era, identification of point mutations and their corresponding genes is increasingly becoming a powerful and important approach to define plant gene function. To perform initial mapping experiments efficiently on Arabidopsis mutants, enrichment of easy-to-use and reliable polymorphic DNA markers would be desirable. We present here a list of InDel polymorphic markers between Col-0 and Ler-0 accessions that can be detected in standard agarose gel electrophoresis. |
format | Online Article Text |
id | pubmed-7355782 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-73557822020-07-23 Easy-to-Use InDel Markers for Genetic Mapping between Col-0 and Ler-0 Accessions of Arabidopsis thaliana Tanaka, Takahiro Nishii, Yuichi Matsuo, Hirotoshi Takahashi, Taku Plants (Basel) Technical Note Map-based gene cloning has played a key role in many genetic studies using the model plant, Arabidopsis thaliana. In the post- next generation sequencing era, identification of point mutations and their corresponding genes is increasingly becoming a powerful and important approach to define plant gene function. To perform initial mapping experiments efficiently on Arabidopsis mutants, enrichment of easy-to-use and reliable polymorphic DNA markers would be desirable. We present here a list of InDel polymorphic markers between Col-0 and Ler-0 accessions that can be detected in standard agarose gel electrophoresis. MDPI 2020-06-22 /pmc/articles/PMC7355782/ /pubmed/32580428 http://dx.doi.org/10.3390/plants9060779 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Technical Note Tanaka, Takahiro Nishii, Yuichi Matsuo, Hirotoshi Takahashi, Taku Easy-to-Use InDel Markers for Genetic Mapping between Col-0 and Ler-0 Accessions of Arabidopsis thaliana |
title | Easy-to-Use InDel Markers for Genetic Mapping between Col-0 and Ler-0 Accessions of Arabidopsis thaliana |
title_full | Easy-to-Use InDel Markers for Genetic Mapping between Col-0 and Ler-0 Accessions of Arabidopsis thaliana |
title_fullStr | Easy-to-Use InDel Markers for Genetic Mapping between Col-0 and Ler-0 Accessions of Arabidopsis thaliana |
title_full_unstemmed | Easy-to-Use InDel Markers for Genetic Mapping between Col-0 and Ler-0 Accessions of Arabidopsis thaliana |
title_short | Easy-to-Use InDel Markers for Genetic Mapping between Col-0 and Ler-0 Accessions of Arabidopsis thaliana |
title_sort | easy-to-use indel markers for genetic mapping between col-0 and ler-0 accessions of arabidopsis thaliana |
topic | Technical Note |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7355782/ https://www.ncbi.nlm.nih.gov/pubmed/32580428 http://dx.doi.org/10.3390/plants9060779 |
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