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Effect of Oral Administration of 1,3-1,6 β-Glucans in DWV Naturally Infected Newly Emerged Bees (Apis mellifera L.)

Honeybee pathogens have an important role in honeybee colony mortality and colony losses; most of them are widely spread and necessitate worldwide solutions to contrast honeybee’s decline. Possible accepted solutions to cope with the spread of honeybee’s pathogens are focused on the study of experim...

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Autores principales: Felicioli, Antonio, Forzan, Mario, Sagona, Simona, D’Agostino, Paola, Baido, Diego, Fronte, Baldassare, Mazzei, Maurizio
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7355867/
https://www.ncbi.nlm.nih.gov/pubmed/32344871
http://dx.doi.org/10.3390/vetsci7020052
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author Felicioli, Antonio
Forzan, Mario
Sagona, Simona
D’Agostino, Paola
Baido, Diego
Fronte, Baldassare
Mazzei, Maurizio
author_facet Felicioli, Antonio
Forzan, Mario
Sagona, Simona
D’Agostino, Paola
Baido, Diego
Fronte, Baldassare
Mazzei, Maurizio
author_sort Felicioli, Antonio
collection PubMed
description Honeybee pathogens have an important role in honeybee colony mortality and colony losses; most of them are widely spread and necessitate worldwide solutions to contrast honeybee’s decline. Possible accepted solutions to cope with the spread of honeybee’s pathogens are focused on the study of experimental protocols to enhance the insect’s immune defenses. Honeybee’s artificial diet capable to stimulate the immune system is a promising field of investigation as ascertained by the introduction of 1,3-1,6 β-glucans as a dietary supplement. In this work, by collecting faecal samples of honeybees exposed to different dietary conditions of 1,3-1,6 β-glucans (0.5% and 2% w/w), it has been possible to investigate the Deformed wing virus (DWV) viral load kinetic without harming the insects. Virological data obtained by a one-step TaqMan RT-PCR highlighted the ability of 1,3-1,6 β-glucans to reduce the viral load at the 24th day of rearing. The results indicated that the diet supplemented with 1,3-1,6 β-glucans was associated with a dose-dependent activation of phenoloxidase. The control group showed a higher survival rate than the experimental groups. This research confirmed 1,3-1,6 β-glucans as molecules able to modulate honeybees’ defense pathways, and this is the first report in which the kinetic of DWV infection in honeybee faeces has been monitored by a RT-qPCR.
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spelling pubmed-73558672020-07-23 Effect of Oral Administration of 1,3-1,6 β-Glucans in DWV Naturally Infected Newly Emerged Bees (Apis mellifera L.) Felicioli, Antonio Forzan, Mario Sagona, Simona D’Agostino, Paola Baido, Diego Fronte, Baldassare Mazzei, Maurizio Vet Sci Article Honeybee pathogens have an important role in honeybee colony mortality and colony losses; most of them are widely spread and necessitate worldwide solutions to contrast honeybee’s decline. Possible accepted solutions to cope with the spread of honeybee’s pathogens are focused on the study of experimental protocols to enhance the insect’s immune defenses. Honeybee’s artificial diet capable to stimulate the immune system is a promising field of investigation as ascertained by the introduction of 1,3-1,6 β-glucans as a dietary supplement. In this work, by collecting faecal samples of honeybees exposed to different dietary conditions of 1,3-1,6 β-glucans (0.5% and 2% w/w), it has been possible to investigate the Deformed wing virus (DWV) viral load kinetic without harming the insects. Virological data obtained by a one-step TaqMan RT-PCR highlighted the ability of 1,3-1,6 β-glucans to reduce the viral load at the 24th day of rearing. The results indicated that the diet supplemented with 1,3-1,6 β-glucans was associated with a dose-dependent activation of phenoloxidase. The control group showed a higher survival rate than the experimental groups. This research confirmed 1,3-1,6 β-glucans as molecules able to modulate honeybees’ defense pathways, and this is the first report in which the kinetic of DWV infection in honeybee faeces has been monitored by a RT-qPCR. MDPI 2020-04-25 /pmc/articles/PMC7355867/ /pubmed/32344871 http://dx.doi.org/10.3390/vetsci7020052 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Felicioli, Antonio
Forzan, Mario
Sagona, Simona
D’Agostino, Paola
Baido, Diego
Fronte, Baldassare
Mazzei, Maurizio
Effect of Oral Administration of 1,3-1,6 β-Glucans in DWV Naturally Infected Newly Emerged Bees (Apis mellifera L.)
title Effect of Oral Administration of 1,3-1,6 β-Glucans in DWV Naturally Infected Newly Emerged Bees (Apis mellifera L.)
title_full Effect of Oral Administration of 1,3-1,6 β-Glucans in DWV Naturally Infected Newly Emerged Bees (Apis mellifera L.)
title_fullStr Effect of Oral Administration of 1,3-1,6 β-Glucans in DWV Naturally Infected Newly Emerged Bees (Apis mellifera L.)
title_full_unstemmed Effect of Oral Administration of 1,3-1,6 β-Glucans in DWV Naturally Infected Newly Emerged Bees (Apis mellifera L.)
title_short Effect of Oral Administration of 1,3-1,6 β-Glucans in DWV Naturally Infected Newly Emerged Bees (Apis mellifera L.)
title_sort effect of oral administration of 1,3-1,6 β-glucans in dwv naturally infected newly emerged bees (apis mellifera l.)
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7355867/
https://www.ncbi.nlm.nih.gov/pubmed/32344871
http://dx.doi.org/10.3390/vetsci7020052
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