Magnetic Solid-Phase Extraction Based on Poly 4-Vinyl Pyridine for HPLC-FLD Analysis of Naproxen in Urine Samples

A magnetic solid phase extraction technique followed by liquid chromatography with a fluorescence detector for naproxen analysis in human urine samples was developed. The method includes the extraction of naproxen with a magnetic solid synthetized with magnetite and poly 4-vinylpriridine, followed b...

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Autores principales: Escamilla-Lara, Karen A., Heredia, Ana C., Peña-Alvarez, Araceli, Ibarra, Israel S., Barrado, Enrique, Rodriguez, Jose A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7355941/
https://www.ncbi.nlm.nih.gov/pubmed/32630475
http://dx.doi.org/10.3390/molecules25122924
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author Escamilla-Lara, Karen A.
Heredia, Ana C.
Peña-Alvarez, Araceli
Ibarra, Israel S.
Barrado, Enrique
Rodriguez, Jose A.
author_facet Escamilla-Lara, Karen A.
Heredia, Ana C.
Peña-Alvarez, Araceli
Ibarra, Israel S.
Barrado, Enrique
Rodriguez, Jose A.
author_sort Escamilla-Lara, Karen A.
collection PubMed
description A magnetic solid phase extraction technique followed by liquid chromatography with a fluorescence detector for naproxen analysis in human urine samples was developed. The method includes the extraction of naproxen with a magnetic solid synthetized with magnetite and poly 4-vinylpriridine, followed by the magnetic separation of the solid phase and desorption of the analyte with methanol. Under optimal conditions, the linear range of the calibration curve was 0.05–0.60 μg L(−1), with a limit of detection of 0.02 μg L(−1). In all cases values of repeatability were lower than 5.0% with recoveries of 99.4 ± 1.3%. Precision and accuracy values are adequate for naproxen (Npx) analysis in urine samples.
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spelling pubmed-73559412020-07-22 Magnetic Solid-Phase Extraction Based on Poly 4-Vinyl Pyridine for HPLC-FLD Analysis of Naproxen in Urine Samples Escamilla-Lara, Karen A. Heredia, Ana C. Peña-Alvarez, Araceli Ibarra, Israel S. Barrado, Enrique Rodriguez, Jose A. Molecules Article A magnetic solid phase extraction technique followed by liquid chromatography with a fluorescence detector for naproxen analysis in human urine samples was developed. The method includes the extraction of naproxen with a magnetic solid synthetized with magnetite and poly 4-vinylpriridine, followed by the magnetic separation of the solid phase and desorption of the analyte with methanol. Under optimal conditions, the linear range of the calibration curve was 0.05–0.60 μg L(−1), with a limit of detection of 0.02 μg L(−1). In all cases values of repeatability were lower than 5.0% with recoveries of 99.4 ± 1.3%. Precision and accuracy values are adequate for naproxen (Npx) analysis in urine samples. MDPI 2020-06-25 /pmc/articles/PMC7355941/ /pubmed/32630475 http://dx.doi.org/10.3390/molecules25122924 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Escamilla-Lara, Karen A.
Heredia, Ana C.
Peña-Alvarez, Araceli
Ibarra, Israel S.
Barrado, Enrique
Rodriguez, Jose A.
Magnetic Solid-Phase Extraction Based on Poly 4-Vinyl Pyridine for HPLC-FLD Analysis of Naproxen in Urine Samples
title Magnetic Solid-Phase Extraction Based on Poly 4-Vinyl Pyridine for HPLC-FLD Analysis of Naproxen in Urine Samples
title_full Magnetic Solid-Phase Extraction Based on Poly 4-Vinyl Pyridine for HPLC-FLD Analysis of Naproxen in Urine Samples
title_fullStr Magnetic Solid-Phase Extraction Based on Poly 4-Vinyl Pyridine for HPLC-FLD Analysis of Naproxen in Urine Samples
title_full_unstemmed Magnetic Solid-Phase Extraction Based on Poly 4-Vinyl Pyridine for HPLC-FLD Analysis of Naproxen in Urine Samples
title_short Magnetic Solid-Phase Extraction Based on Poly 4-Vinyl Pyridine for HPLC-FLD Analysis of Naproxen in Urine Samples
title_sort magnetic solid-phase extraction based on poly 4-vinyl pyridine for hplc-fld analysis of naproxen in urine samples
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7355941/
https://www.ncbi.nlm.nih.gov/pubmed/32630475
http://dx.doi.org/10.3390/molecules25122924
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