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Biocompatibility of Bone Marrow-Derived Mesenchymal Stem Cells in the Rat Inner Ear following Trans-Tympanic Administration
Recent advancements in stem cell therapy have led to an increased interest within the auditory community in exploring the potential of mesenchymal stem cells (MSCs) in the treatment of inner ear disorders. However, the biocompatibility of MSCs with the inner ear, especially when delivered non-surgic...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7355977/ https://www.ncbi.nlm.nih.gov/pubmed/32498432 http://dx.doi.org/10.3390/jcm9061711 |
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author | Eshraghi, Adrien A. Ocak, Emre Zhu, Angela Mittal, Jeenu Davies, Camron Shahal, David Bulut, Erdogan Sinha, Rahul Shah, Viraj Perdomo, Mario M. Mittal, Rahul |
author_facet | Eshraghi, Adrien A. Ocak, Emre Zhu, Angela Mittal, Jeenu Davies, Camron Shahal, David Bulut, Erdogan Sinha, Rahul Shah, Viraj Perdomo, Mario M. Mittal, Rahul |
author_sort | Eshraghi, Adrien A. |
collection | PubMed |
description | Recent advancements in stem cell therapy have led to an increased interest within the auditory community in exploring the potential of mesenchymal stem cells (MSCs) in the treatment of inner ear disorders. However, the biocompatibility of MSCs with the inner ear, especially when delivered non-surgically and in the immunocompetent cochlea, is not completely understood. In this study, we determined the effect of intratympanic administration of rodent bone marrow MSCs (BM-MSCs) on the inner ear in an immunocompetent rat model. The administration of MSCs did not lead to the generation of any oxidative stress in the rat inner ear. There was no significant production of proinflammatory cytokines, tumor necrosis factor (TNF)-α, interleukin (IL)-1β, IL-6 and IL-12, due to BM-MSCs administration into the rat cochlea. BM-MSCs do not activate caspase 3 pathway, which plays a central role in sensory cell damage. Additionally, transferase dUTP nick end labeling (TUNEL) staining determined that there was no significant cell death associated with the administration of BM-MSCs. The results of the present study suggest that trans-tympanic administration of BM-MSCs does not result in oxidative stress or inflammatory response in the immunocompetent rat cochlea. |
format | Online Article Text |
id | pubmed-7355977 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-73559772020-07-22 Biocompatibility of Bone Marrow-Derived Mesenchymal Stem Cells in the Rat Inner Ear following Trans-Tympanic Administration Eshraghi, Adrien A. Ocak, Emre Zhu, Angela Mittal, Jeenu Davies, Camron Shahal, David Bulut, Erdogan Sinha, Rahul Shah, Viraj Perdomo, Mario M. Mittal, Rahul J Clin Med Article Recent advancements in stem cell therapy have led to an increased interest within the auditory community in exploring the potential of mesenchymal stem cells (MSCs) in the treatment of inner ear disorders. However, the biocompatibility of MSCs with the inner ear, especially when delivered non-surgically and in the immunocompetent cochlea, is not completely understood. In this study, we determined the effect of intratympanic administration of rodent bone marrow MSCs (BM-MSCs) on the inner ear in an immunocompetent rat model. The administration of MSCs did not lead to the generation of any oxidative stress in the rat inner ear. There was no significant production of proinflammatory cytokines, tumor necrosis factor (TNF)-α, interleukin (IL)-1β, IL-6 and IL-12, due to BM-MSCs administration into the rat cochlea. BM-MSCs do not activate caspase 3 pathway, which plays a central role in sensory cell damage. Additionally, transferase dUTP nick end labeling (TUNEL) staining determined that there was no significant cell death associated with the administration of BM-MSCs. The results of the present study suggest that trans-tympanic administration of BM-MSCs does not result in oxidative stress or inflammatory response in the immunocompetent rat cochlea. MDPI 2020-06-02 /pmc/articles/PMC7355977/ /pubmed/32498432 http://dx.doi.org/10.3390/jcm9061711 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Eshraghi, Adrien A. Ocak, Emre Zhu, Angela Mittal, Jeenu Davies, Camron Shahal, David Bulut, Erdogan Sinha, Rahul Shah, Viraj Perdomo, Mario M. Mittal, Rahul Biocompatibility of Bone Marrow-Derived Mesenchymal Stem Cells in the Rat Inner Ear following Trans-Tympanic Administration |
title | Biocompatibility of Bone Marrow-Derived Mesenchymal Stem Cells in the Rat Inner Ear following Trans-Tympanic Administration |
title_full | Biocompatibility of Bone Marrow-Derived Mesenchymal Stem Cells in the Rat Inner Ear following Trans-Tympanic Administration |
title_fullStr | Biocompatibility of Bone Marrow-Derived Mesenchymal Stem Cells in the Rat Inner Ear following Trans-Tympanic Administration |
title_full_unstemmed | Biocompatibility of Bone Marrow-Derived Mesenchymal Stem Cells in the Rat Inner Ear following Trans-Tympanic Administration |
title_short | Biocompatibility of Bone Marrow-Derived Mesenchymal Stem Cells in the Rat Inner Ear following Trans-Tympanic Administration |
title_sort | biocompatibility of bone marrow-derived mesenchymal stem cells in the rat inner ear following trans-tympanic administration |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7355977/ https://www.ncbi.nlm.nih.gov/pubmed/32498432 http://dx.doi.org/10.3390/jcm9061711 |
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