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The Epidemiological Characteristics of the Korean Bat Paramyxovirus between 2016 and 2019

Bats are considered reservoirs of severe emerging human pathogens. Notably, bats host major mammalian paramyxoviruses from the family Paramyxoviridae, order Mononegavirales. In this study, paramyxoviruses were investigated by reverse transcription semi-nested polymerase chain reaction (RT-semi-neste...

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Autores principales: Jang, Seong Sik, Noh, Ji Yeong, Lo, Van Thi, Choi, Yong Gun, Yoon, Sun-Woo, Jeong, Dae Gwin, Kim, Hye Kwon
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7356101/
https://www.ncbi.nlm.nih.gov/pubmed/32512880
http://dx.doi.org/10.3390/microorganisms8060844
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author Jang, Seong Sik
Noh, Ji Yeong
Lo, Van Thi
Choi, Yong Gun
Yoon, Sun-Woo
Jeong, Dae Gwin
Kim, Hye Kwon
author_facet Jang, Seong Sik
Noh, Ji Yeong
Lo, Van Thi
Choi, Yong Gun
Yoon, Sun-Woo
Jeong, Dae Gwin
Kim, Hye Kwon
author_sort Jang, Seong Sik
collection PubMed
description Bats are considered reservoirs of severe emerging human pathogens. Notably, bats host major mammalian paramyxoviruses from the family Paramyxoviridae, order Mononegavirales. In this study, paramyxoviruses were investigated by reverse transcription semi-nested polymerase chain reaction (RT-semi-nested PCR) and reverse transcription polymerase chain reaction (RT-PCR), based on the RT-semi-nested PCR using the consensus paramyxovirus primers targeting the RNA dependent-RNA-polymerase (RdRp) region. In addition, RT-PCR was performed using newly designed primers targeting regions of the fusion protein (F) and hemagglutinin-neuraminidase (HN). The dominant bat species in the collection site of paramyxoviruses were Miniopterus schreibersii, Myotis macrodactylus, Myotis petax, and Rhinolophus ferrumequinum. Paramyxoviruses were detected in four samples in 2016 and six in 2019. Meanwhile, in samples collected in 2017 and 2018, no paramyxoviruses were detected. Phylogenetic analysis based on the partial nucleotide sequences of RdRp, F, and HN proteins suggested that the viruses belonged to the proposed genus Shaanvirus. In conclusion, this study revealed that bat paramyxoviruses in Korea belonged to a single genus and circulated sporadically in several provinces, including Chungbuk, Gangwon, Jeju, and Jeonnam.
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spelling pubmed-73561012020-07-31 The Epidemiological Characteristics of the Korean Bat Paramyxovirus between 2016 and 2019 Jang, Seong Sik Noh, Ji Yeong Lo, Van Thi Choi, Yong Gun Yoon, Sun-Woo Jeong, Dae Gwin Kim, Hye Kwon Microorganisms Article Bats are considered reservoirs of severe emerging human pathogens. Notably, bats host major mammalian paramyxoviruses from the family Paramyxoviridae, order Mononegavirales. In this study, paramyxoviruses were investigated by reverse transcription semi-nested polymerase chain reaction (RT-semi-nested PCR) and reverse transcription polymerase chain reaction (RT-PCR), based on the RT-semi-nested PCR using the consensus paramyxovirus primers targeting the RNA dependent-RNA-polymerase (RdRp) region. In addition, RT-PCR was performed using newly designed primers targeting regions of the fusion protein (F) and hemagglutinin-neuraminidase (HN). The dominant bat species in the collection site of paramyxoviruses were Miniopterus schreibersii, Myotis macrodactylus, Myotis petax, and Rhinolophus ferrumequinum. Paramyxoviruses were detected in four samples in 2016 and six in 2019. Meanwhile, in samples collected in 2017 and 2018, no paramyxoviruses were detected. Phylogenetic analysis based on the partial nucleotide sequences of RdRp, F, and HN proteins suggested that the viruses belonged to the proposed genus Shaanvirus. In conclusion, this study revealed that bat paramyxoviruses in Korea belonged to a single genus and circulated sporadically in several provinces, including Chungbuk, Gangwon, Jeju, and Jeonnam. MDPI 2020-06-04 /pmc/articles/PMC7356101/ /pubmed/32512880 http://dx.doi.org/10.3390/microorganisms8060844 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Jang, Seong Sik
Noh, Ji Yeong
Lo, Van Thi
Choi, Yong Gun
Yoon, Sun-Woo
Jeong, Dae Gwin
Kim, Hye Kwon
The Epidemiological Characteristics of the Korean Bat Paramyxovirus between 2016 and 2019
title The Epidemiological Characteristics of the Korean Bat Paramyxovirus between 2016 and 2019
title_full The Epidemiological Characteristics of the Korean Bat Paramyxovirus between 2016 and 2019
title_fullStr The Epidemiological Characteristics of the Korean Bat Paramyxovirus between 2016 and 2019
title_full_unstemmed The Epidemiological Characteristics of the Korean Bat Paramyxovirus between 2016 and 2019
title_short The Epidemiological Characteristics of the Korean Bat Paramyxovirus between 2016 and 2019
title_sort epidemiological characteristics of the korean bat paramyxovirus between 2016 and 2019
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7356101/
https://www.ncbi.nlm.nih.gov/pubmed/32512880
http://dx.doi.org/10.3390/microorganisms8060844
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