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Quantitative Profiling of Arabidopsis Polar Glycerolipids under Two Types of Heat Stress
At the cellular level, the remodelling of membrane lipids and production of heat shock proteins are the two main strategies whereby plants survive heat stress. Although many studies related to glycerolipids and HSPs under heat stress have been reported separately, detailed alterations of glycerolipi...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7356150/ https://www.ncbi.nlm.nih.gov/pubmed/32485906 http://dx.doi.org/10.3390/plants9060693 |
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author | Qin, Feng Lin, Liang Jia, Yanxia Li, Weiqi Yu, Buzhu |
author_facet | Qin, Feng Lin, Liang Jia, Yanxia Li, Weiqi Yu, Buzhu |
author_sort | Qin, Feng |
collection | PubMed |
description | At the cellular level, the remodelling of membrane lipids and production of heat shock proteins are the two main strategies whereby plants survive heat stress. Although many studies related to glycerolipids and HSPs under heat stress have been reported separately, detailed alterations of glycerolipids and the role of HSPs in the alterations of glycerolipids still need to be revealed. In this study, we profiled the glycerolipids of wild-type Arabidopsis and its HSP101-deficient mutant hot-1 under two types of heat stress. Our results demonstrated that the alterations of glycerolipids were very similar in wild-type Arabidopsis and hot-1 during heat stress. Although heat acclimation led to a slight decrease of glycerolipids, the decrease of glycerolipids in plants without heat acclimation is more severe under heat shock. The contents of 36:x monogalactosyl diacylglycerol (MGDG) were slightly increased, whereas that of 34:6 MGDG and 34:4 phosphatidylglycerol (PG) were severely decreased during moderate heat stress. Our findings suggested that heat acclimation could reduce the degradation of glycerolipids under heat shock. Synthesis of glycerolipids through the prokaryotic pathway was severely suppressed, whereas that through the eukaryotic pathway was slightly enhanced during moderate heat stress. In addition, HSP101 has a minor effect on the alterations of glycerolipids under heat stress. |
format | Online Article Text |
id | pubmed-7356150 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-73561502020-07-31 Quantitative Profiling of Arabidopsis Polar Glycerolipids under Two Types of Heat Stress Qin, Feng Lin, Liang Jia, Yanxia Li, Weiqi Yu, Buzhu Plants (Basel) Article At the cellular level, the remodelling of membrane lipids and production of heat shock proteins are the two main strategies whereby plants survive heat stress. Although many studies related to glycerolipids and HSPs under heat stress have been reported separately, detailed alterations of glycerolipids and the role of HSPs in the alterations of glycerolipids still need to be revealed. In this study, we profiled the glycerolipids of wild-type Arabidopsis and its HSP101-deficient mutant hot-1 under two types of heat stress. Our results demonstrated that the alterations of glycerolipids were very similar in wild-type Arabidopsis and hot-1 during heat stress. Although heat acclimation led to a slight decrease of glycerolipids, the decrease of glycerolipids in plants without heat acclimation is more severe under heat shock. The contents of 36:x monogalactosyl diacylglycerol (MGDG) were slightly increased, whereas that of 34:6 MGDG and 34:4 phosphatidylglycerol (PG) were severely decreased during moderate heat stress. Our findings suggested that heat acclimation could reduce the degradation of glycerolipids under heat shock. Synthesis of glycerolipids through the prokaryotic pathway was severely suppressed, whereas that through the eukaryotic pathway was slightly enhanced during moderate heat stress. In addition, HSP101 has a minor effect on the alterations of glycerolipids under heat stress. MDPI 2020-05-29 /pmc/articles/PMC7356150/ /pubmed/32485906 http://dx.doi.org/10.3390/plants9060693 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Qin, Feng Lin, Liang Jia, Yanxia Li, Weiqi Yu, Buzhu Quantitative Profiling of Arabidopsis Polar Glycerolipids under Two Types of Heat Stress |
title | Quantitative Profiling of Arabidopsis Polar Glycerolipids under Two Types of Heat Stress |
title_full | Quantitative Profiling of Arabidopsis Polar Glycerolipids under Two Types of Heat Stress |
title_fullStr | Quantitative Profiling of Arabidopsis Polar Glycerolipids under Two Types of Heat Stress |
title_full_unstemmed | Quantitative Profiling of Arabidopsis Polar Glycerolipids under Two Types of Heat Stress |
title_short | Quantitative Profiling of Arabidopsis Polar Glycerolipids under Two Types of Heat Stress |
title_sort | quantitative profiling of arabidopsis polar glycerolipids under two types of heat stress |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7356150/ https://www.ncbi.nlm.nih.gov/pubmed/32485906 http://dx.doi.org/10.3390/plants9060693 |
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