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Metabolomics of Myrcia bella Populations in Brazilian Savanna Reveals Strong Influence of Environmental Factors on Its Specialized Metabolism

Environmental conditions influence specialized plant metabolism. However, many studies aiming to understand these modulations have been conducted with model plants and/or under controlled conditions, thus not reflecting the complex interaction between plants and environment. To fully grasp these int...

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Autores principales: Saldanha, Luiz Leonardo, Allard, Pierre-Marie, Afzan, Adlin, de Melo, Fernanda Pereira de Souza Rosa, Marcourt, Laurence, Queiroz, Emerson Ferreira, Vilegas, Wagner, Furlan, Cláudia Maria, Dokkedal, Anne Lígia, Wolfender, Jean-Luc
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7356273/
https://www.ncbi.nlm.nih.gov/pubmed/32604974
http://dx.doi.org/10.3390/molecules25122954
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author Saldanha, Luiz Leonardo
Allard, Pierre-Marie
Afzan, Adlin
de Melo, Fernanda Pereira de Souza Rosa
Marcourt, Laurence
Queiroz, Emerson Ferreira
Vilegas, Wagner
Furlan, Cláudia Maria
Dokkedal, Anne Lígia
Wolfender, Jean-Luc
author_facet Saldanha, Luiz Leonardo
Allard, Pierre-Marie
Afzan, Adlin
de Melo, Fernanda Pereira de Souza Rosa
Marcourt, Laurence
Queiroz, Emerson Ferreira
Vilegas, Wagner
Furlan, Cláudia Maria
Dokkedal, Anne Lígia
Wolfender, Jean-Luc
author_sort Saldanha, Luiz Leonardo
collection PubMed
description Environmental conditions influence specialized plant metabolism. However, many studies aiming to understand these modulations have been conducted with model plants and/or under controlled conditions, thus not reflecting the complex interaction between plants and environment. To fully grasp these interactions, we investigated the specialized metabolism and genetic diversity of a native plant in its natural environment. We chose Myrcia bella due to its medicinal interest and occurrence in Brazilian savanna regions with diverse climate and soil conditions. An LC-HRMS-based metabolomics approach was applied to analyze 271 samples harvested across seven regions during the dry and rainy season. Genetic diversity was assessed in a subset of 40 samples using amplified fragment length polymorphism. Meteorological factors including rainfall, temperature, radiation, humidity, and soil nutrient and mineral composition were recorded in each region and correlated with chemical variation through multivariate analysis (MVDA). Marker compounds were selected using a statistically informed molecular network and annotated by dereplication against an in silico database of natural products. The integrated results evidenced different chemotypes, with variation in flavonoid and tannin content mainly linked to soil conditions. Different levels of genetic diversity and distance of populations were found to be correlated with the identified chemotypes. These observations and the proposed analytical workflow contribute to the global understanding of the impact of abiotic factors and genotype on the accumulation of given metabolites and, therefore, could be valuable to guide further medicinal exploration of native species.
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spelling pubmed-73562732020-07-31 Metabolomics of Myrcia bella Populations in Brazilian Savanna Reveals Strong Influence of Environmental Factors on Its Specialized Metabolism Saldanha, Luiz Leonardo Allard, Pierre-Marie Afzan, Adlin de Melo, Fernanda Pereira de Souza Rosa Marcourt, Laurence Queiroz, Emerson Ferreira Vilegas, Wagner Furlan, Cláudia Maria Dokkedal, Anne Lígia Wolfender, Jean-Luc Molecules Article Environmental conditions influence specialized plant metabolism. However, many studies aiming to understand these modulations have been conducted with model plants and/or under controlled conditions, thus not reflecting the complex interaction between plants and environment. To fully grasp these interactions, we investigated the specialized metabolism and genetic diversity of a native plant in its natural environment. We chose Myrcia bella due to its medicinal interest and occurrence in Brazilian savanna regions with diverse climate and soil conditions. An LC-HRMS-based metabolomics approach was applied to analyze 271 samples harvested across seven regions during the dry and rainy season. Genetic diversity was assessed in a subset of 40 samples using amplified fragment length polymorphism. Meteorological factors including rainfall, temperature, radiation, humidity, and soil nutrient and mineral composition were recorded in each region and correlated with chemical variation through multivariate analysis (MVDA). Marker compounds were selected using a statistically informed molecular network and annotated by dereplication against an in silico database of natural products. The integrated results evidenced different chemotypes, with variation in flavonoid and tannin content mainly linked to soil conditions. Different levels of genetic diversity and distance of populations were found to be correlated with the identified chemotypes. These observations and the proposed analytical workflow contribute to the global understanding of the impact of abiotic factors and genotype on the accumulation of given metabolites and, therefore, could be valuable to guide further medicinal exploration of native species. MDPI 2020-06-26 /pmc/articles/PMC7356273/ /pubmed/32604974 http://dx.doi.org/10.3390/molecules25122954 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Saldanha, Luiz Leonardo
Allard, Pierre-Marie
Afzan, Adlin
de Melo, Fernanda Pereira de Souza Rosa
Marcourt, Laurence
Queiroz, Emerson Ferreira
Vilegas, Wagner
Furlan, Cláudia Maria
Dokkedal, Anne Lígia
Wolfender, Jean-Luc
Metabolomics of Myrcia bella Populations in Brazilian Savanna Reveals Strong Influence of Environmental Factors on Its Specialized Metabolism
title Metabolomics of Myrcia bella Populations in Brazilian Savanna Reveals Strong Influence of Environmental Factors on Its Specialized Metabolism
title_full Metabolomics of Myrcia bella Populations in Brazilian Savanna Reveals Strong Influence of Environmental Factors on Its Specialized Metabolism
title_fullStr Metabolomics of Myrcia bella Populations in Brazilian Savanna Reveals Strong Influence of Environmental Factors on Its Specialized Metabolism
title_full_unstemmed Metabolomics of Myrcia bella Populations in Brazilian Savanna Reveals Strong Influence of Environmental Factors on Its Specialized Metabolism
title_short Metabolomics of Myrcia bella Populations in Brazilian Savanna Reveals Strong Influence of Environmental Factors on Its Specialized Metabolism
title_sort metabolomics of myrcia bella populations in brazilian savanna reveals strong influence of environmental factors on its specialized metabolism
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7356273/
https://www.ncbi.nlm.nih.gov/pubmed/32604974
http://dx.doi.org/10.3390/molecules25122954
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