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Effects of Joint Lavage with Dimethylsulfoxide on LPS-Induced Synovitis in Horses—Clinical and Laboratorial Aspects

Several studies in human and equine medicine have produced controversial results regarding the role of dimethylsulfoxide (DMSO) as a therapeutic agent. This study aimed to evaluate the effect of joint lavage with different DMSO concentrations on biomarkers of synovial fluid inflammation and cartilag...

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Autores principales: Sotelo, Eric Danilo Pauls, Vendruscolo, Cynthia Prado, Fülber, Joice, Seidel, Sarah Raphaela Torquato, Jaramillo, Fernando Mosquera, Agreste, Fernanda Rodrigues, da Silva, Luís Cláudio Lopes Correia, Baccarin, Raquel Yvonne Arantes
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
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Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7356314/
https://www.ncbi.nlm.nih.gov/pubmed/32365982
http://dx.doi.org/10.3390/vetsci7020057
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author Sotelo, Eric Danilo Pauls
Vendruscolo, Cynthia Prado
Fülber, Joice
Seidel, Sarah Raphaela Torquato
Jaramillo, Fernando Mosquera
Agreste, Fernanda Rodrigues
da Silva, Luís Cláudio Lopes Correia
Baccarin, Raquel Yvonne Arantes
author_facet Sotelo, Eric Danilo Pauls
Vendruscolo, Cynthia Prado
Fülber, Joice
Seidel, Sarah Raphaela Torquato
Jaramillo, Fernando Mosquera
Agreste, Fernanda Rodrigues
da Silva, Luís Cláudio Lopes Correia
Baccarin, Raquel Yvonne Arantes
author_sort Sotelo, Eric Danilo Pauls
collection PubMed
description Several studies in human and equine medicine have produced controversial results regarding the role of dimethylsulfoxide (DMSO) as a therapeutic agent. This study aimed to evaluate the effect of joint lavage with different DMSO concentrations on biomarkers of synovial fluid inflammation and cartilage degradation in joints with lipopolysaccharide (LPS)-induced synovitis. Twenty-six tibiotarsal joints of 13 horses were randomly distributed into four groups (lactated Ringer’s solution; 5% DMSO in lactated Ringer’s; 10% DMSO in lactated Ringer’s; and sham). All animals were evaluated for the presence of lameness, and synovial fluid analyses were performed at 0 h, 1 h, 8 h, 24 h, and 48 h (T0, T1, T8, T24, and T48, respectively). The white blood cell counts (WBC), total protein (TP), urea, prostaglandin E2 (PGE2), interleukin (IL)-1β, IL-6, IL-10, tumor necrosis factor-α (TNF-α), hyaluronic acid (HA), and chondroitin sulfate (CS) concentrations were measured. The WBC counts and PGE2, IL-1β, IL-6, and TP concentrations increased in all groups at T8 compared to baseline values (p < 0.05). At T48, only the 5% DMSO and 10% DMSO groups showed a significant decrease in WBC counts (p < 0.05). Furthermore, the 10% DMSO group had lower concentrations of PGE2 and IL-1β at T48 than at T8 (p < 0.05) and presented lower IL-6 levels than the5% DMSO and lactated Ringer’s groups at T24. All groups showed an increase in CS concentration after LPS-induced synovitis. Joint lavage with 10% DMSO in lactated Ringer’s has anti-inflammatory but not chondroprotective effects.
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spelling pubmed-73563142020-07-31 Effects of Joint Lavage with Dimethylsulfoxide on LPS-Induced Synovitis in Horses—Clinical and Laboratorial Aspects Sotelo, Eric Danilo Pauls Vendruscolo, Cynthia Prado Fülber, Joice Seidel, Sarah Raphaela Torquato Jaramillo, Fernando Mosquera Agreste, Fernanda Rodrigues da Silva, Luís Cláudio Lopes Correia Baccarin, Raquel Yvonne Arantes Vet Sci Article Several studies in human and equine medicine have produced controversial results regarding the role of dimethylsulfoxide (DMSO) as a therapeutic agent. This study aimed to evaluate the effect of joint lavage with different DMSO concentrations on biomarkers of synovial fluid inflammation and cartilage degradation in joints with lipopolysaccharide (LPS)-induced synovitis. Twenty-six tibiotarsal joints of 13 horses were randomly distributed into four groups (lactated Ringer’s solution; 5% DMSO in lactated Ringer’s; 10% DMSO in lactated Ringer’s; and sham). All animals were evaluated for the presence of lameness, and synovial fluid analyses were performed at 0 h, 1 h, 8 h, 24 h, and 48 h (T0, T1, T8, T24, and T48, respectively). The white blood cell counts (WBC), total protein (TP), urea, prostaglandin E2 (PGE2), interleukin (IL)-1β, IL-6, IL-10, tumor necrosis factor-α (TNF-α), hyaluronic acid (HA), and chondroitin sulfate (CS) concentrations were measured. The WBC counts and PGE2, IL-1β, IL-6, and TP concentrations increased in all groups at T8 compared to baseline values (p < 0.05). At T48, only the 5% DMSO and 10% DMSO groups showed a significant decrease in WBC counts (p < 0.05). Furthermore, the 10% DMSO group had lower concentrations of PGE2 and IL-1β at T48 than at T8 (p < 0.05) and presented lower IL-6 levels than the5% DMSO and lactated Ringer’s groups at T24. All groups showed an increase in CS concentration after LPS-induced synovitis. Joint lavage with 10% DMSO in lactated Ringer’s has anti-inflammatory but not chondroprotective effects. MDPI 2020-04-30 /pmc/articles/PMC7356314/ /pubmed/32365982 http://dx.doi.org/10.3390/vetsci7020057 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Sotelo, Eric Danilo Pauls
Vendruscolo, Cynthia Prado
Fülber, Joice
Seidel, Sarah Raphaela Torquato
Jaramillo, Fernando Mosquera
Agreste, Fernanda Rodrigues
da Silva, Luís Cláudio Lopes Correia
Baccarin, Raquel Yvonne Arantes
Effects of Joint Lavage with Dimethylsulfoxide on LPS-Induced Synovitis in Horses—Clinical and Laboratorial Aspects
title Effects of Joint Lavage with Dimethylsulfoxide on LPS-Induced Synovitis in Horses—Clinical and Laboratorial Aspects
title_full Effects of Joint Lavage with Dimethylsulfoxide on LPS-Induced Synovitis in Horses—Clinical and Laboratorial Aspects
title_fullStr Effects of Joint Lavage with Dimethylsulfoxide on LPS-Induced Synovitis in Horses—Clinical and Laboratorial Aspects
title_full_unstemmed Effects of Joint Lavage with Dimethylsulfoxide on LPS-Induced Synovitis in Horses—Clinical and Laboratorial Aspects
title_short Effects of Joint Lavage with Dimethylsulfoxide on LPS-Induced Synovitis in Horses—Clinical and Laboratorial Aspects
title_sort effects of joint lavage with dimethylsulfoxide on lps-induced synovitis in horses—clinical and laboratorial aspects
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7356314/
https://www.ncbi.nlm.nih.gov/pubmed/32365982
http://dx.doi.org/10.3390/vetsci7020057
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