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Circulating Inflammatory miRNAs Associated with Parkinson’s Disease Pathophysiology

Parkinson’s disease (PD) is the second most common neurodegenerative disease worldwide, being largely characterized by motor features. MicroRNAs (miRNAs) are small non-coding RNAs, whose deregulation has been associated with neurodegeneration in PD. In this study, miRNAs targeting cell death and/or...

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Autores principales: Oliveira, Sara R., Dionísio, Pedro A., Correia Guedes, Leonor, Gonçalves, Nilza, Coelho, Miguel, Rosa, Mário M., Amaral, Joana D., Ferreira, Joaquim J., Rodrigues, Cecília M. P.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7356527/
https://www.ncbi.nlm.nih.gov/pubmed/32585840
http://dx.doi.org/10.3390/biom10060945
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author Oliveira, Sara R.
Dionísio, Pedro A.
Correia Guedes, Leonor
Gonçalves, Nilza
Coelho, Miguel
Rosa, Mário M.
Amaral, Joana D.
Ferreira, Joaquim J.
Rodrigues, Cecília M. P.
author_facet Oliveira, Sara R.
Dionísio, Pedro A.
Correia Guedes, Leonor
Gonçalves, Nilza
Coelho, Miguel
Rosa, Mário M.
Amaral, Joana D.
Ferreira, Joaquim J.
Rodrigues, Cecília M. P.
author_sort Oliveira, Sara R.
collection PubMed
description Parkinson’s disease (PD) is the second most common neurodegenerative disease worldwide, being largely characterized by motor features. MicroRNAs (miRNAs) are small non-coding RNAs, whose deregulation has been associated with neurodegeneration in PD. In this study, miRNAs targeting cell death and/or inflammation pathways were selected and their expression compared in the serum of PD patients and healthy controls. We used two independent cohorts (discovery and validation) of 20 idiopathic PD patients (iPD) and 20 healthy controls each. We also analyzed an additional group of 45 patients with a mutation in the leucine-rich repeat kinase 2 (LRRK2) gene (LRRK2-PD). miRNA expression was determined using Taqman qRT-PCR and their performance to discriminate between groups was assessed by receiver operating characteristic (ROC) curve analysis. We found miR-146a, miR-335-3p, and miR-335-5p downregulated in iPD and LRRK2-PD patients versus controls in both cohorts. In addition, miR-155 was upregulated in LRRK2-PD compared to iPD patients showing an appropriate value of area under the ROC curve (AUC 0.80) to discriminate between the two groups. In conclusion, our study identified a panel of inflammatory related miRNAs differentially expressed between PD patients and healthy controls that highlight key pathophysiological processes and may contribute to improve disease diagnosis.
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spelling pubmed-73565272020-07-30 Circulating Inflammatory miRNAs Associated with Parkinson’s Disease Pathophysiology Oliveira, Sara R. Dionísio, Pedro A. Correia Guedes, Leonor Gonçalves, Nilza Coelho, Miguel Rosa, Mário M. Amaral, Joana D. Ferreira, Joaquim J. Rodrigues, Cecília M. P. Biomolecules Article Parkinson’s disease (PD) is the second most common neurodegenerative disease worldwide, being largely characterized by motor features. MicroRNAs (miRNAs) are small non-coding RNAs, whose deregulation has been associated with neurodegeneration in PD. In this study, miRNAs targeting cell death and/or inflammation pathways were selected and their expression compared in the serum of PD patients and healthy controls. We used two independent cohorts (discovery and validation) of 20 idiopathic PD patients (iPD) and 20 healthy controls each. We also analyzed an additional group of 45 patients with a mutation in the leucine-rich repeat kinase 2 (LRRK2) gene (LRRK2-PD). miRNA expression was determined using Taqman qRT-PCR and their performance to discriminate between groups was assessed by receiver operating characteristic (ROC) curve analysis. We found miR-146a, miR-335-3p, and miR-335-5p downregulated in iPD and LRRK2-PD patients versus controls in both cohorts. In addition, miR-155 was upregulated in LRRK2-PD compared to iPD patients showing an appropriate value of area under the ROC curve (AUC 0.80) to discriminate between the two groups. In conclusion, our study identified a panel of inflammatory related miRNAs differentially expressed between PD patients and healthy controls that highlight key pathophysiological processes and may contribute to improve disease diagnosis. MDPI 2020-06-23 /pmc/articles/PMC7356527/ /pubmed/32585840 http://dx.doi.org/10.3390/biom10060945 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Oliveira, Sara R.
Dionísio, Pedro A.
Correia Guedes, Leonor
Gonçalves, Nilza
Coelho, Miguel
Rosa, Mário M.
Amaral, Joana D.
Ferreira, Joaquim J.
Rodrigues, Cecília M. P.
Circulating Inflammatory miRNAs Associated with Parkinson’s Disease Pathophysiology
title Circulating Inflammatory miRNAs Associated with Parkinson’s Disease Pathophysiology
title_full Circulating Inflammatory miRNAs Associated with Parkinson’s Disease Pathophysiology
title_fullStr Circulating Inflammatory miRNAs Associated with Parkinson’s Disease Pathophysiology
title_full_unstemmed Circulating Inflammatory miRNAs Associated with Parkinson’s Disease Pathophysiology
title_short Circulating Inflammatory miRNAs Associated with Parkinson’s Disease Pathophysiology
title_sort circulating inflammatory mirnas associated with parkinson’s disease pathophysiology
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7356527/
https://www.ncbi.nlm.nih.gov/pubmed/32585840
http://dx.doi.org/10.3390/biom10060945
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