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Cationic Dendrimer G2-S16 Inhibits Herpes Simplex Type 2 Infection and Protects Mice Vaginal Microbiome
The G2-S16 polyanionic carbosilane dendrimer is a promising microbicide that inhibits HSV-2 infection in vitro and in vivo in mice models. This G2-S16 dendrimer inhibits HSV-2 infection even in the presence of semen. Murine models, such as BALB/c female mice, are generally used to characterize host-...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7356682/ https://www.ncbi.nlm.nih.gov/pubmed/32512836 http://dx.doi.org/10.3390/pharmaceutics12060515 |
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author | Guerrero-Beltrán, Carlos Garcia-Heredia, Inmaculada Ceña-Diez, Rafael Rodriguez-Izquierdo, Ignacio Serramía, María Jesús Martinez-Hernandez, Francisco Lluesma-Gomez, Mónica Martinez-Garcia, Manuel Muñoz-Fernández, María Ángeles |
author_facet | Guerrero-Beltrán, Carlos Garcia-Heredia, Inmaculada Ceña-Diez, Rafael Rodriguez-Izquierdo, Ignacio Serramía, María Jesús Martinez-Hernandez, Francisco Lluesma-Gomez, Mónica Martinez-Garcia, Manuel Muñoz-Fernández, María Ángeles |
author_sort | Guerrero-Beltrán, Carlos |
collection | PubMed |
description | The G2-S16 polyanionic carbosilane dendrimer is a promising microbicide that inhibits HSV-2 infection in vitro and in vivo in mice models. This G2-S16 dendrimer inhibits HSV-2 infection even in the presence of semen. Murine models, such as BALB/c female mice, are generally used to characterize host-pathogen interactions within the vaginal tract. However, the composition of endogenous vaginal flora remains largely undefined with modern microbiome analyses. It is important to note that the G2-S16 dendrimer does not change healthy mouse vaginal microbiome where Pseudomonas (10.2–79.1%) and Janthinobacterium (0.7–13%) are the more abundant genera. The HSV-2 vaginally infected female mice showed a significant microbiome alteration because an increase of Staphylococcus (up to 98.8%) and Escherichia (30.76%) levels were observed becoming these bacteria the predominant genera. BALB/c female mice vaginally-treated with the G2-S16 dendrimer and infected with the HSV-2 maintained a healthy vaginal microbiome similar to uninfected female mice. Summarizing, the G2-S16 polyanionic carbosilane dendrimer inhibits the HSV-2 infection in the presence of semen and prevents the alteration of mice female vaginal microbiome. |
format | Online Article Text |
id | pubmed-7356682 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-73566822020-07-22 Cationic Dendrimer G2-S16 Inhibits Herpes Simplex Type 2 Infection and Protects Mice Vaginal Microbiome Guerrero-Beltrán, Carlos Garcia-Heredia, Inmaculada Ceña-Diez, Rafael Rodriguez-Izquierdo, Ignacio Serramía, María Jesús Martinez-Hernandez, Francisco Lluesma-Gomez, Mónica Martinez-Garcia, Manuel Muñoz-Fernández, María Ángeles Pharmaceutics Article The G2-S16 polyanionic carbosilane dendrimer is a promising microbicide that inhibits HSV-2 infection in vitro and in vivo in mice models. This G2-S16 dendrimer inhibits HSV-2 infection even in the presence of semen. Murine models, such as BALB/c female mice, are generally used to characterize host-pathogen interactions within the vaginal tract. However, the composition of endogenous vaginal flora remains largely undefined with modern microbiome analyses. It is important to note that the G2-S16 dendrimer does not change healthy mouse vaginal microbiome where Pseudomonas (10.2–79.1%) and Janthinobacterium (0.7–13%) are the more abundant genera. The HSV-2 vaginally infected female mice showed a significant microbiome alteration because an increase of Staphylococcus (up to 98.8%) and Escherichia (30.76%) levels were observed becoming these bacteria the predominant genera. BALB/c female mice vaginally-treated with the G2-S16 dendrimer and infected with the HSV-2 maintained a healthy vaginal microbiome similar to uninfected female mice. Summarizing, the G2-S16 polyanionic carbosilane dendrimer inhibits the HSV-2 infection in the presence of semen and prevents the alteration of mice female vaginal microbiome. MDPI 2020-06-04 /pmc/articles/PMC7356682/ /pubmed/32512836 http://dx.doi.org/10.3390/pharmaceutics12060515 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Guerrero-Beltrán, Carlos Garcia-Heredia, Inmaculada Ceña-Diez, Rafael Rodriguez-Izquierdo, Ignacio Serramía, María Jesús Martinez-Hernandez, Francisco Lluesma-Gomez, Mónica Martinez-Garcia, Manuel Muñoz-Fernández, María Ángeles Cationic Dendrimer G2-S16 Inhibits Herpes Simplex Type 2 Infection and Protects Mice Vaginal Microbiome |
title | Cationic Dendrimer G2-S16 Inhibits Herpes Simplex Type 2 Infection and Protects Mice Vaginal Microbiome |
title_full | Cationic Dendrimer G2-S16 Inhibits Herpes Simplex Type 2 Infection and Protects Mice Vaginal Microbiome |
title_fullStr | Cationic Dendrimer G2-S16 Inhibits Herpes Simplex Type 2 Infection and Protects Mice Vaginal Microbiome |
title_full_unstemmed | Cationic Dendrimer G2-S16 Inhibits Herpes Simplex Type 2 Infection and Protects Mice Vaginal Microbiome |
title_short | Cationic Dendrimer G2-S16 Inhibits Herpes Simplex Type 2 Infection and Protects Mice Vaginal Microbiome |
title_sort | cationic dendrimer g2-s16 inhibits herpes simplex type 2 infection and protects mice vaginal microbiome |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7356682/ https://www.ncbi.nlm.nih.gov/pubmed/32512836 http://dx.doi.org/10.3390/pharmaceutics12060515 |
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