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Cationic Dendrimer G2-S16 Inhibits Herpes Simplex Type 2 Infection and Protects Mice Vaginal Microbiome

The G2-S16 polyanionic carbosilane dendrimer is a promising microbicide that inhibits HSV-2 infection in vitro and in vivo in mice models. This G2-S16 dendrimer inhibits HSV-2 infection even in the presence of semen. Murine models, such as BALB/c female mice, are generally used to characterize host-...

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Autores principales: Guerrero-Beltrán, Carlos, Garcia-Heredia, Inmaculada, Ceña-Diez, Rafael, Rodriguez-Izquierdo, Ignacio, Serramía, María Jesús, Martinez-Hernandez, Francisco, Lluesma-Gomez, Mónica, Martinez-Garcia, Manuel, Muñoz-Fernández, María Ángeles
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7356682/
https://www.ncbi.nlm.nih.gov/pubmed/32512836
http://dx.doi.org/10.3390/pharmaceutics12060515
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author Guerrero-Beltrán, Carlos
Garcia-Heredia, Inmaculada
Ceña-Diez, Rafael
Rodriguez-Izquierdo, Ignacio
Serramía, María Jesús
Martinez-Hernandez, Francisco
Lluesma-Gomez, Mónica
Martinez-Garcia, Manuel
Muñoz-Fernández, María Ángeles
author_facet Guerrero-Beltrán, Carlos
Garcia-Heredia, Inmaculada
Ceña-Diez, Rafael
Rodriguez-Izquierdo, Ignacio
Serramía, María Jesús
Martinez-Hernandez, Francisco
Lluesma-Gomez, Mónica
Martinez-Garcia, Manuel
Muñoz-Fernández, María Ángeles
author_sort Guerrero-Beltrán, Carlos
collection PubMed
description The G2-S16 polyanionic carbosilane dendrimer is a promising microbicide that inhibits HSV-2 infection in vitro and in vivo in mice models. This G2-S16 dendrimer inhibits HSV-2 infection even in the presence of semen. Murine models, such as BALB/c female mice, are generally used to characterize host-pathogen interactions within the vaginal tract. However, the composition of endogenous vaginal flora remains largely undefined with modern microbiome analyses. It is important to note that the G2-S16 dendrimer does not change healthy mouse vaginal microbiome where Pseudomonas (10.2–79.1%) and Janthinobacterium (0.7–13%) are the more abundant genera. The HSV-2 vaginally infected female mice showed a significant microbiome alteration because an increase of Staphylococcus (up to 98.8%) and Escherichia (30.76%) levels were observed becoming these bacteria the predominant genera. BALB/c female mice vaginally-treated with the G2-S16 dendrimer and infected with the HSV-2 maintained a healthy vaginal microbiome similar to uninfected female mice. Summarizing, the G2-S16 polyanionic carbosilane dendrimer inhibits the HSV-2 infection in the presence of semen and prevents the alteration of mice female vaginal microbiome.
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spelling pubmed-73566822020-07-22 Cationic Dendrimer G2-S16 Inhibits Herpes Simplex Type 2 Infection and Protects Mice Vaginal Microbiome Guerrero-Beltrán, Carlos Garcia-Heredia, Inmaculada Ceña-Diez, Rafael Rodriguez-Izquierdo, Ignacio Serramía, María Jesús Martinez-Hernandez, Francisco Lluesma-Gomez, Mónica Martinez-Garcia, Manuel Muñoz-Fernández, María Ángeles Pharmaceutics Article The G2-S16 polyanionic carbosilane dendrimer is a promising microbicide that inhibits HSV-2 infection in vitro and in vivo in mice models. This G2-S16 dendrimer inhibits HSV-2 infection even in the presence of semen. Murine models, such as BALB/c female mice, are generally used to characterize host-pathogen interactions within the vaginal tract. However, the composition of endogenous vaginal flora remains largely undefined with modern microbiome analyses. It is important to note that the G2-S16 dendrimer does not change healthy mouse vaginal microbiome where Pseudomonas (10.2–79.1%) and Janthinobacterium (0.7–13%) are the more abundant genera. The HSV-2 vaginally infected female mice showed a significant microbiome alteration because an increase of Staphylococcus (up to 98.8%) and Escherichia (30.76%) levels were observed becoming these bacteria the predominant genera. BALB/c female mice vaginally-treated with the G2-S16 dendrimer and infected with the HSV-2 maintained a healthy vaginal microbiome similar to uninfected female mice. Summarizing, the G2-S16 polyanionic carbosilane dendrimer inhibits the HSV-2 infection in the presence of semen and prevents the alteration of mice female vaginal microbiome. MDPI 2020-06-04 /pmc/articles/PMC7356682/ /pubmed/32512836 http://dx.doi.org/10.3390/pharmaceutics12060515 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Guerrero-Beltrán, Carlos
Garcia-Heredia, Inmaculada
Ceña-Diez, Rafael
Rodriguez-Izquierdo, Ignacio
Serramía, María Jesús
Martinez-Hernandez, Francisco
Lluesma-Gomez, Mónica
Martinez-Garcia, Manuel
Muñoz-Fernández, María Ángeles
Cationic Dendrimer G2-S16 Inhibits Herpes Simplex Type 2 Infection and Protects Mice Vaginal Microbiome
title Cationic Dendrimer G2-S16 Inhibits Herpes Simplex Type 2 Infection and Protects Mice Vaginal Microbiome
title_full Cationic Dendrimer G2-S16 Inhibits Herpes Simplex Type 2 Infection and Protects Mice Vaginal Microbiome
title_fullStr Cationic Dendrimer G2-S16 Inhibits Herpes Simplex Type 2 Infection and Protects Mice Vaginal Microbiome
title_full_unstemmed Cationic Dendrimer G2-S16 Inhibits Herpes Simplex Type 2 Infection and Protects Mice Vaginal Microbiome
title_short Cationic Dendrimer G2-S16 Inhibits Herpes Simplex Type 2 Infection and Protects Mice Vaginal Microbiome
title_sort cationic dendrimer g2-s16 inhibits herpes simplex type 2 infection and protects mice vaginal microbiome
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7356682/
https://www.ncbi.nlm.nih.gov/pubmed/32512836
http://dx.doi.org/10.3390/pharmaceutics12060515
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