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Rpv Mediated Defense Responses in Grapevine Offspring Resistant to Plasmopara viticola

Downy mildew, caused by the biotrophic oomycete Plasmopara viticola, is one of the most serious grapevine diseases. The development of new varieties, showing partial resistance to downy mildew, through traditional breeding provides a sustainable and effective solution for disease management. Marker-...

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Detalles Bibliográficos
Autores principales: Possamai, Tyrone, Migliaro, Daniele, Gardiman, Massimo, Velasco, Riccardo, De Nardi, Barbara
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7356695/
https://www.ncbi.nlm.nih.gov/pubmed/32580445
http://dx.doi.org/10.3390/plants9060781
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author Possamai, Tyrone
Migliaro, Daniele
Gardiman, Massimo
Velasco, Riccardo
De Nardi, Barbara
author_facet Possamai, Tyrone
Migliaro, Daniele
Gardiman, Massimo
Velasco, Riccardo
De Nardi, Barbara
author_sort Possamai, Tyrone
collection PubMed
description Downy mildew, caused by the biotrophic oomycete Plasmopara viticola, is one of the most serious grapevine diseases. The development of new varieties, showing partial resistance to downy mildew, through traditional breeding provides a sustainable and effective solution for disease management. Marker-assisted-selection (MAS) provide fast and cost-effective genotyping methods, but phenotyping remains necessary to characterize the host–pathogen interaction and assess the effective resistance level of new varieties as well as to validate MAS selection. In this study, the Rpv mediated defense responses were investigated in 31 genotypes, encompassing susceptible and resistant varieties and 26 seedlings, following inoculation of leaf discs with P. viticola. The offspring differed in Rpv loci inherited (none, one or two): Rpv3-3 and Rpv10 from Solaris and Rpv3-1 and Rpv12 from Kozma 20-3. To improve the assessment of different resistance responses, pathogen reaction (sporulation) and host reaction (necrosis) were scored separately as independent features. They were differently expressed depending on Rpv locus: offspring carrying Rpv3-1 and Rpv12 loci showed the strongest resistance response (scarce sporulation and necrosis), those carrying Rpv3-3 locus showed the highest levels of necrosis while Rpv10 carrying genotypes showed intermediate levels of both sporulation and necrosis.
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spelling pubmed-73566952020-07-22 Rpv Mediated Defense Responses in Grapevine Offspring Resistant to Plasmopara viticola Possamai, Tyrone Migliaro, Daniele Gardiman, Massimo Velasco, Riccardo De Nardi, Barbara Plants (Basel) Technical Note Downy mildew, caused by the biotrophic oomycete Plasmopara viticola, is one of the most serious grapevine diseases. The development of new varieties, showing partial resistance to downy mildew, through traditional breeding provides a sustainable and effective solution for disease management. Marker-assisted-selection (MAS) provide fast and cost-effective genotyping methods, but phenotyping remains necessary to characterize the host–pathogen interaction and assess the effective resistance level of new varieties as well as to validate MAS selection. In this study, the Rpv mediated defense responses were investigated in 31 genotypes, encompassing susceptible and resistant varieties and 26 seedlings, following inoculation of leaf discs with P. viticola. The offspring differed in Rpv loci inherited (none, one or two): Rpv3-3 and Rpv10 from Solaris and Rpv3-1 and Rpv12 from Kozma 20-3. To improve the assessment of different resistance responses, pathogen reaction (sporulation) and host reaction (necrosis) were scored separately as independent features. They were differently expressed depending on Rpv locus: offspring carrying Rpv3-1 and Rpv12 loci showed the strongest resistance response (scarce sporulation and necrosis), those carrying Rpv3-3 locus showed the highest levels of necrosis while Rpv10 carrying genotypes showed intermediate levels of both sporulation and necrosis. MDPI 2020-06-22 /pmc/articles/PMC7356695/ /pubmed/32580445 http://dx.doi.org/10.3390/plants9060781 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Technical Note
Possamai, Tyrone
Migliaro, Daniele
Gardiman, Massimo
Velasco, Riccardo
De Nardi, Barbara
Rpv Mediated Defense Responses in Grapevine Offspring Resistant to Plasmopara viticola
title Rpv Mediated Defense Responses in Grapevine Offspring Resistant to Plasmopara viticola
title_full Rpv Mediated Defense Responses in Grapevine Offspring Resistant to Plasmopara viticola
title_fullStr Rpv Mediated Defense Responses in Grapevine Offspring Resistant to Plasmopara viticola
title_full_unstemmed Rpv Mediated Defense Responses in Grapevine Offspring Resistant to Plasmopara viticola
title_short Rpv Mediated Defense Responses in Grapevine Offspring Resistant to Plasmopara viticola
title_sort rpv mediated defense responses in grapevine offspring resistant to plasmopara viticola
topic Technical Note
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7356695/
https://www.ncbi.nlm.nih.gov/pubmed/32580445
http://dx.doi.org/10.3390/plants9060781
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