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Gold Nanoparticles as Colorimetric Sensors for the Detection of DNA Bases and Related Compounds

Results regarding interaction of colloidal gold solutions with nucleobases, including uracil (U), as well as its sulfur derivatives, 2-thiouracil (2TU) and 4-thiouracil (4TU), cytosine (C), adenine (A), and guanine (G), as well as urea and thiourea (TU), are reported. Anionic stabilized citrate gold...

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Autor principal: Iglesias, Emilia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7356728/
https://www.ncbi.nlm.nih.gov/pubmed/32586064
http://dx.doi.org/10.3390/molecules25122890
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author Iglesias, Emilia
author_facet Iglesias, Emilia
author_sort Iglesias, Emilia
collection PubMed
description Results regarding interaction of colloidal gold solutions with nucleobases, including uracil (U), as well as its sulfur derivatives, 2-thiouracil (2TU) and 4-thiouracil (4TU), cytosine (C), adenine (A), and guanine (G), as well as urea and thiourea (TU), are reported. Anionic stabilized citrate gold nanoparticles (AuNPs) were synthesized by reducing the tetrachloroaurate (III) trihydrate with trisodium citrate. The surface plasmon resonance (SPR) band was used in the characterization of synthesized AuNPs, as well as transmission electron microscope (TEM) imaging, which was used in the characterization of dispersed and aggregated gold nanoparticles. Interactions of nucleobases with the gold surface was analyzed by following the plasmon absorbance band red shift of the AuNPs. The sulfur-containing compounds adsorbed to the nanoparticle surfaces by chemisorption-type interactions; with TU and 4TU, the process is accompanied by a sudden change in color; in contrast, 2TU forms stable functionalized gold nanoparticles. Urea and U do not adsorb to nanoparticle surfaces, but the other heterocyclic bases containing nitrogen interact effectively with the gold surface, causing the assembly of nanoparticles, even though the interparticle self-aggregation process was slower than that mediated by either TU or 4TU. The method is efficient in the colorimetric detection of nucleobases and derivatives at concentration levels on the order of 1 µM.
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spelling pubmed-73567282020-07-22 Gold Nanoparticles as Colorimetric Sensors for the Detection of DNA Bases and Related Compounds Iglesias, Emilia Molecules Article Results regarding interaction of colloidal gold solutions with nucleobases, including uracil (U), as well as its sulfur derivatives, 2-thiouracil (2TU) and 4-thiouracil (4TU), cytosine (C), adenine (A), and guanine (G), as well as urea and thiourea (TU), are reported. Anionic stabilized citrate gold nanoparticles (AuNPs) were synthesized by reducing the tetrachloroaurate (III) trihydrate with trisodium citrate. The surface plasmon resonance (SPR) band was used in the characterization of synthesized AuNPs, as well as transmission electron microscope (TEM) imaging, which was used in the characterization of dispersed and aggregated gold nanoparticles. Interactions of nucleobases with the gold surface was analyzed by following the plasmon absorbance band red shift of the AuNPs. The sulfur-containing compounds adsorbed to the nanoparticle surfaces by chemisorption-type interactions; with TU and 4TU, the process is accompanied by a sudden change in color; in contrast, 2TU forms stable functionalized gold nanoparticles. Urea and U do not adsorb to nanoparticle surfaces, but the other heterocyclic bases containing nitrogen interact effectively with the gold surface, causing the assembly of nanoparticles, even though the interparticle self-aggregation process was slower than that mediated by either TU or 4TU. The method is efficient in the colorimetric detection of nucleobases and derivatives at concentration levels on the order of 1 µM. MDPI 2020-06-23 /pmc/articles/PMC7356728/ /pubmed/32586064 http://dx.doi.org/10.3390/molecules25122890 Text en © 2020 by the author. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Iglesias, Emilia
Gold Nanoparticles as Colorimetric Sensors for the Detection of DNA Bases and Related Compounds
title Gold Nanoparticles as Colorimetric Sensors for the Detection of DNA Bases and Related Compounds
title_full Gold Nanoparticles as Colorimetric Sensors for the Detection of DNA Bases and Related Compounds
title_fullStr Gold Nanoparticles as Colorimetric Sensors for the Detection of DNA Bases and Related Compounds
title_full_unstemmed Gold Nanoparticles as Colorimetric Sensors for the Detection of DNA Bases and Related Compounds
title_short Gold Nanoparticles as Colorimetric Sensors for the Detection of DNA Bases and Related Compounds
title_sort gold nanoparticles as colorimetric sensors for the detection of dna bases and related compounds
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7356728/
https://www.ncbi.nlm.nih.gov/pubmed/32586064
http://dx.doi.org/10.3390/molecules25122890
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